Determination of Fluorescence Polarization and Absorption Anisotropy in Molecular Complexes Having Threefold Rotational Symmetry

The current work concerns investigation of the polarization properties of complex molecular ensembles exhibiting threefold (C3) rotational symmetry, particularly with regard to the interplay between their structure and dynamics of internal energy transfer. We assume that the molecules or chromophores in such complexes possess strongly overlapped spectra both for absorption and fluorescence. Such trimeric structures are widely found in biological preparations, as for example the trimer of C-phycocyanin (C-PC). Higher order aggregates, e.g. hex-amers and three-hexamer rods, are also investigated and compared with the trimer case. The theory addresses both steady-state and 8-pulse excitation and establishes some links between them. Monochromophoric, bichro-mophoric and trichromophoric molecular complexes are individually examined. For steady-state excitation, analytical formulas are reported for the degree of fluorescence polarization and absorption anisotropy. It is shown that the polarization is dependent on the chromophore inclination relative to the symmetry axis, the relative efficiencies of absorption and fluorescence by chromophores of different spectral types, and the rates of energy equilibration. To assess the validity of the theory, it has been applied to C-PC aggregates. Here it was found that different C-PC aggregates provide practically identical polarization response. For S-pulse excitation we give analytical formulas for determination of the fluorescence depolarization, and also the depolarization associated with absorption recovery, both for a monochromophoric trimer and some particular cases of bichromophoric trimer. More complicated systems are analyzed by computer modeling. Thus it transpires that the initial polarization anisotropy r(t = 0) takes the value 0.4 for all considered aggregates; the long-time limit r(t →∞) has about the same value as is associated with steady-state excitation. We also show that with steady-state excitation the degree of fluorescence polarization is practically equal for various C3 aggregates of C-PC, and that the major factor determining the polarization is the chromophore orientation relative to the symmetry axis

Spectral Inversion of Multi-Line Full-Disk Observations of Quiet Sun Magnetic Fields

Spectral inversion codes are powerful tools to analyze spectropolarimetric observations, and they provide important diagnostics of solar magnetic fields. Inversion codes differ by numerical procedures, approximations of the atmospheric model, and description of radiative transfer. Stokes Inversion based on Response functions (SIR) is an implementation widely used by the solar physics community. It allows to work with different atmospheric components, where gradients of different physical parameters are possible, e.g., magnetic field strength and velocities. The spectropolarimetric full-disk observations were carried out with the Stokesmeter of the Solar Telescope for Operative Predictions (STOP) at the Sayan Observatory on 3 February 2009, when neither an active region nor any other extended flux concentration was present on the Sun. In this study of quiet Sun magnetic fields, we apply the SIR code simultaneously to 15 spectral lines. A tendency is found that weaker magnetic field strengths occur closer to the limb. We explain this finding by the fact that close to the limb, we are more sensitive to higher altitudes in an expanding flux tube, where the field strength should be smaller since the magnetic flux is conserved with height. Typically, the inversions deliver two populations of magnetic elements: (1) high magnetic field strengths (1500-2000 G) and high temperatures (5500-6500 K) and (2) weak magnetic fields (50-150 G) and low temperatures (5000-5300 K).Comment: 10 pages, 6 figures, accepted for Solar Physic