70 research outputs found
Effect of coenzyme Q10 on ascorbic acid, vitamin E, and coenzyme Q concentrations in testes of rats chronically exposed to cadmium
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Chronic treatment with CdCl2 and accumulation of cadmium in the liver, kidneys, testes and skeletal muscle of rats
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Effect of coenzyme Q10 on ascorbic acid, vitamin E, and coenzyme Q concentrations in testes of rats chronically exposed to cadmium
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Antioxidant effect of coenzyme Q10 in blood from cadmium-exposed rats
The effects of acute exposure to cadmium (Cd) on the blood antioxidant defense system (AOS), lipid peroxide (LP) concentration and hematological parameters, and the possible protective role of coenzyme Q10 (CoQ10) was studied. Male Wistar albino rats 3 months old were treated with cadmium as CdCl2 (0,4mg Cd/kg b.m., i.p., 24h before the sacrificing) or with coenzyme Q10 + Cd (20mg CoQ10/kg b.m., i.m., 48h + 0,4 mg Cd/kg b.m., i.p., 24h before the sacrificing). The hematological parameters: red blood cells count (RBCs), hemoglobin (Hb) concentration and hematocrite (Ht) value were significantly decreased in the blood of Cd treated rats. Intoxication with Cd was also followed by significantly increased of LP concentration. We also observed increased concentrations of non-enzymatic components of antioxidant defense system (AOS): reduced glutathione (GSH), vitamin C (Vit C) and vitamin E (Vit E). Pretreatment with CoQ10 exhibited a protective role on the toxic effects of Cd on the hematological values, LP concentration as well as on endogeneous antioxidant components.Cilj ovog rada je bio da se ispita uticaj kadmijuma (Cd) na antioksidacioni zaÅ”titni sistem (AOS), koncentraciju lipidnih peroksida (LP) i hematoloÅ”ke parametre u krvi, kao i zaÅ”titna uloga koenzima Q10 (CoQ10). Mužjaci pacova Wistar albino, stari tri meseca, akutno su tretirani kadmijumom (0,4mg Cd/kg t.m., i.p., 24h pre žrtvovanja) i koenzimom Q10 + Cd (20mg CoQ10/kg t.m., i.m., 48h + 0,4mg Cd/kg t.m., 24h pre žrtvovanja). HematoloÅ”ki parametri: broj eritrocita (RBCs), koncentracija hemoglobina (Hb) i hematoloÅ”ka vrednost (Ht) su znaÄajno smanjeni u krvi pacova posle tretmana kadmijumom. Cd znaÄajno poveÄava i koncentraciju LP, kao i koncentracije neenzimskih komponenti AOS-a: redukovani glutation (GSH), vitamin C (Vit C) i vitamin E (Vit E). Eksperimenti sa pacovima koji su izazvanu anemiju i oksidaciona oÅ”teÄenja (smanjuje koncentraciju LP), kao i znaÄajno umanjuje toksiÄne efekte Cd na komponente AOS-a.nul
Cadmium-induced changes in haemato-biochemical parameters, lipid peroxidation and glutathione content in blood of rats
Cadmium (Cd+2) is an ubiquitous toxic metal that may induce oxidative damage by disturbing the prooxidant-antioxidant balance in the blood. Wistar albino rats (3 months old), were injected with a single dose of CdCh (0.4 mg Cd/kg i.p. and sacrificed after 24h). The hematological parameters: red blood cells count (RBCs), hematocrite (Ht) value and hemoglobin (Hb) concentration were significantly decreased in the blood of Cd treated rats. The activities of enzymes alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in plasma, as well as concentration of blood glucose were significantly increased in animals treated with cadmium in comparison to control values. The treatment with Cd increased lipid peroxidation (LP) and reduced glutathione (GSH) contents in the blood, suggesting that the Cd induced oxidative stress. These results of our study suggested that Cd induced alterations in hemato-biochemical parameters and LP and GSH content.Kadmijum (Cd+2) je Å”iroko rasprostranjen toksiÄan metal koji može izazvati oksidaciona oÅ”teÄenja u krvi remeteÄi prooksidaciono-antioksidacionu ravnotežu. Wistar albino pacovi (stari 3 meseca), tretirani su pojedinaÄnim dozama CdCl; (0.4 mg Cd/kg i.p) i žrtvovani posle 24 Äasa. HematoloÅ”ki parametri, i to: broj eritrocita (RBCs), hematokritska vredno.st (Ht) i koncentracija hemoglobina (Hb) znaÄajno su smanjeni u krvi tretiranih pacova. Aktivnost enzima alanin aminotransaminaze (ALT) i aspartat aminotransaminaze (AST) u plazmi, kao i koncentracija glukoze u krvi znaÄajno su poveÄani kod životinja tretiranih kadmijumom u poreÄenju sa kontrolnim vrednostima. Tretman sa kadmijumom, takoÄe je doveo do poveÄanja lipidne peroksidacije (LP) i redukovanog glutationa (GSH). Sve to ukazuje da je kadmijum izazvao oksidacioni stres u krvi pacova. Dobijeni rezultati ukazuju da je Cd indukovao promenĆ© u hemato-biohemijskim parametrima, kao i u sadržaju LP i GSH u krvi tretiranih pacova.nul
A possible protective role of coenzyme Q10 on antioxidant defense enzyme activities in kidneys of rats treated with cadmium
The aim of this study is to establish the effects of cadmium (Cd), coenzyme Q10 (CoQ10) and Cd + CoQ10 on the activities of: Superoxide dismutases (total SOD, manganese containing, Mn SOD, copper zinc containing, CuZn SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione-S-transferase (GST), as well as glutathione reductase (GR) in the kidneys of male Wistar albino rats in comparison with control animals. Significantly increased activities of Tot SOD and CuZn SOD in the kidneys of Cd-treated animals were observed. At the same time, the activity of Mn SOD was significantly decreased in the kidneys of animals treated with Cd, as well as with CoQ10. The activity of CAT was significantly lower in rats exposed to CoQ10 and Cd+CoQ10 in respect to the control animals. GSH-Px and GST activities were significantly decreased in all treated groups of animals (Cd, CoQ10 and Cd+CoQ10), while GR activity was not changed. Presented results reveal, that CoQ10 only partially reversed Cd-induced changes of antioxidant defense enzyme activities in kidney by reversing the effect of Cd on Tot SOD and CuZn SOD activities.Cilj ovog rada bio je da se objasni efekat kadmijuma (Cd), koenzima Q10 (CoQ10) i Cd + CoQ10 na aktivnost: superoksid dismutaza (ukupne, Uk SOD, mangan sadržavajuÄe, Mn SOD, bakar cink sadržavajuÄe, CuZn SOD, katalaze (CAT), glutation peroksidaze (GSH-Px), glutation-S-transferaze (GST) kao i glutation reduktaze (GR) u bubrezima mužjaka Wistar albino pacova u poreÄenju sa kontrolnom grupom životinja. Dobijeni rezultati pokazuju znaÄajno poveÄanje aktivnosti Uk SOD i CuZn SOD u bubrezima pacova tretiranih sa Cd. U isto vreme aktivnost Mn SOD je bila znaÄajno smanjena u bubrezima pacova tretiranih sa Cd, kao i tretiranih sa Cd+CoQ10. U poreÄenju sa kontrolnim životinjama, aktivnost CAT je bila znaÄajno smanjena u bubrezima pacova tretiranih sa CoQ10, kao i tretiranih kombinacijom Cd+CoQ10. Aktivnosti GSH-Px i GST su bile znaÄajno smanjene u bubrezima svih tretiranih grupa životinja (Cd, CoQ10 i Cd+CoQ10), dok se aktivnost GR nije statistiÄki znaÄajno menjala. Iz prikazanih rezultata može se zakljuÄiti, da CoQ10 samo delimiÄno utiÄe na promenĆ© aktivnosti antioksidacionih zaÅ”titnih enzima nastale pod uticajem Cd, kao i da vraÄa aktivnosti Uk SOD i CuZn SOD na normalne vrednosti.nul
A possible protective role of coenzyme Q10 on antioxidant defense enzyme activities in kidneys of rats treated with cadmium
The aim of this study is to establish the effects of cadmium (Cd), coenzyme Q10 (CoQ10) and Cd + CoQ10 on the activities of: Superoxide dismutases (total SOD, manganese containing, Mn SOD, copper zinc containing, CuZn SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione-S-transferase (GST), as well as glutathione reductase (GR) in the kidneys of male Wistar albino rats in comparison with control animals. Significantly increased activities of Tot SOD and CuZn SOD in the kidneys of Cd-treated animals were observed. At the same time, the activity of Mn SOD was significantly decreased in the kidneys of animals treated with Cd, as well as with CoQ10. The activity of CAT was significantly lower in rats exposed to CoQ10 and Cd+CoQ10 in respect to the control animals. GSH-Px and GST activities were significantly decreased in all treated groups of animals (Cd, CoQ10 and Cd+CoQ10), while GR activity was not changed. Presented results reveal, that CoQ10 only partially reversed Cd-induced changes of antioxidant defense enzyme activities in kidney by reversing the effect of Cd on Tot SOD and CuZn SOD activities.Cilj ovog rada bio je da se objasni efekat kadmijuma (Cd), koenzima Q10 (CoQ10) i Cd + CoQ10 na aktivnost: superoksid dismutaza (ukupne, Uk SOD, mangan sadržavajuÄe, Mn SOD, bakar cink sadržavajuÄe, CuZn SOD, katalaze (CAT), glutation peroksidaze (GSH-Px), glutation-S-transferaze (GST) kao i glutation reduktaze (GR) u bubrezima mužjaka Wistar albino pacova u poreÄenju sa kontrolnom grupom životinja. Dobijeni rezultati pokazuju znaÄajno poveÄanje aktivnosti Uk SOD i CuZn SOD u bubrezima pacova tretiranih sa Cd. U isto vreme aktivnost Mn SOD je bila znaÄajno smanjena u bubrezima pacova tretiranih sa Cd, kao i tretiranih sa Cd+CoQ10. U poreÄenju sa kontrolnim životinjama, aktivnost CAT je bila znaÄajno smanjena u bubrezima pacova tretiranih sa CoQ10, kao i tretiranih kombinacijom Cd+CoQ10. Aktivnosti GSH-Px i GST su bile znaÄajno smanjene u bubrezima svih tretiranih grupa životinja (Cd, CoQ10 i Cd+CoQ10), dok se aktivnost GR nije statistiÄki znaÄajno menjala. Iz prikazanih rezultata može se zakljuÄiti, da CoQ10 samo delimiÄno utiÄe na promenĆ© aktivnosti antioksidacionih zaÅ”titnih enzima nastale pod uticajem Cd, kao i da vraÄa aktivnosti Uk SOD i CuZn SOD na normalne vrednosti.nul
Metabolism of sodium nitroprusside (SNP) in rat red blood cells
Sodium nitroprusside (SNP) is an established drug, clinically used in the treatment of hypertensive emergencies. The aim of this study is further elucidation of the molecular mechanism of the SNP action, particularly its metabolism in rat erythrocytes and reticulocytes. Rats erythrocyte and reticulocyte-rich suspensions were aerobically incubated without (control) or in the presence of SNP (0.1,0.25,0.5,1.0 and 1.5 mM). The concentrations of reactive nitrogen species (RNS) and reactive oxygen species (ROS) were determined after incubation. In rat erythrocytes, SNP did not alter nitrite level (NO+ ion indicator), while significantly increased concentrations of hydroxylamine (NO- ion indicator), S-nitrosothiols (SNO) and 3-nitrotyrosine (peroxynitrite indicator). Concentration of superoxide anion (O2-) decreased in the presence of low doses of SNP only, while level of hydrogen peroxide (H2O2) increased in dose-dependent manner in rat erythrocytes. On the other hand, SNP significantly increased nitrite, hydroxylamine and 3-nitrotyrosine concentrations in rat reticulocytes. In addition, low doses of SNP induced decrease of O2- level. Concentration of H2O2 did not alter in rat reticulocytes. On the basis of these data, we can conclude: SNP spontaneously liberated nitric oxide as NO- ion in rat erythrocytes and reticulocytes. In addition, applied experimental doses of SNP induced strong nitrosative and oxidative stress in these cells.Natrijum nitroprusid (SNP) je lek koji se kliniÄki koristi u tretmanu hipertenzija. Cilj ovog rada je da se objasne molekularni mehanizmi delovanja SNPa, sa posebnim akcentom na metabolizam ovog leka u eritrocitima i retikulocitima pacova. Suspenzije eritrocita i crvenih krvnih Äelija bogate retikulocitima su aerobno inkubirane bez (kontrola) ili u prisustvu razliÄitih koncentracija SNPa (0.1, 0.25, 0.5, 1.0 i 1.5 mM). Koncentracije reaktivnih vrsta azota (RNS) i kiseonika (ROS) su odreÄivane nakon inkubacije. U eritrocitima pacova, SNP ne menja koncentraciju nitrita (indikator NO+ jona), dok znaÄajno poveÄava koncentracije hidroksilamina (indicator NO- jona), S-nitrozotiola (SNO) and 3-nitrotirozina (indikator peroksinitrita). Koncentracija superoksid anjon radikala (O2-) je smanjena samo u prisustvu niskih doza SNP, dok je nivo vodonik peroksida (H2O2) poveÄan na dozno-zavisan naÄin u eritrocitima pacova. S druge strane, SNP znaÄajno poveÄava koncentracije nitrita, hidroksilamina i 3-nitrotirozina u retikulocitima pacova. Niske doze SNPa indukuju smanjenje O2- nivoa. Koncentracije H2O2 nisu promenjenje u retikulocitima pacova. Na osnovu iznetih podataka, možemo zakljuÄiti: SNP spontano oslobaÄa azot monoksid kao NO" jon u eritrocitima i retikulocitima pacova. Primenjene eksperimentalne doze SNPa indukuju snažan nitrozacioni i oksidacioni stres u ovim Äelijama.nul
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