Cadmium-induced changes in haemato-biochemical parameters, lipid peroxidation and glutathione content in blood of rats

Abstract

Cadmium (Cd+2) is an ubiquitous toxic metal that may induce oxidative damage by disturbing the prooxidant-antioxidant balance in the blood. Wistar albino rats (3 months old), were injected with a single dose of CdCh (0.4 mg Cd/kg i.p. and sacrificed after 24h). The hematological parameters: red blood cells count (RBCs), hematocrite (Ht) value and hemoglobin (Hb) concentration were significantly decreased in the blood of Cd treated rats. The activities of enzymes alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in plasma, as well as concentration of blood glucose were significantly increased in animals treated with cadmium in comparison to control values. The treatment with Cd increased lipid peroxidation (LP) and reduced glutathione (GSH) contents in the blood, suggesting that the Cd induced oxidative stress. These results of our study suggested that Cd induced alterations in hemato-biochemical parameters and LP and GSH content.Kadmijum (Cd+2) je široko rasprostranjen toksičan metal koji može izazvati oksidaciona oštećenja u krvi remeteći prooksidaciono-antioksidacionu ravnotežu. Wistar albino pacovi (stari 3 meseca), tretirani su pojedinačnim dozama CdCl; (0.4 mg Cd/kg i.p) i žrtvovani posle 24 časa. Hematološki parametri, i to: broj eritrocita (RBCs), hematokritska vredno.st (Ht) i koncentracija hemoglobina (Hb) značajno su smanjeni u krvi tretiranih pacova. Aktivnost enzima alanin aminotransaminaze (ALT) i aspartat aminotransaminaze (AST) u plazmi, kao i koncentracija glukoze u krvi značajno su povećani kod životinja tretiranih kadmijumom u poređenju sa kontrolnim vrednostima. Tretman sa kadmijumom, takođe je doveo do povećanja lipidne peroksidacije (LP) i redukovanog glutationa (GSH). Sve to ukazuje da je kadmijum izazvao oksidacioni stres u krvi pacova. Dobijeni rezultati ukazuju da je Cd indukovao promené u hemato-biohemijskim parametrima, kao i u sadržaju LP i GSH u krvi tretiranih pacova.nul