Glutathione status in the blood of rats after reticulocytosis induced by phenylhydrazine and bleeding

In this experiment, we compared the in vivo effects of phenylhydrazine (PHZ) and bleeding treatment on the redox status and glutathione antioxidative mechanism parameters in the plasma and red blood cells (RBC) of rats. Results showed a lower level of reactive oxygen species (ROS), a higher level of lipid peroxidation and the effective antioxidative role of the glutathione system in the blood of bleeding rats. PHZ-treatment induced higher concentrations of ROS and an accumulation of oxidized glutathione in the plasma, while the glutathione system showed a satisfactory antioxidative capacity in the RBC of rats. When comparing the two anemic groups, the PHZ-treated rats showed marked oxidative stress in the plasma.Cilj ovog rada je bio da se isprate in vivo efekti fenilhidrazinom (PHZ) i krvarenjem indukovane retikulocitoze na parametre redoks i glutationskog antioksidativnog statusa u plazmi i crvenim krvnim ćelijama (RBC) pacova. Rezultati pokazuju niži nivo reaktivnih vrsta kiseonika (ROS), viši nivo lipidne peroksidacije i efikasnu antioksidacionu ulogu glutationskog sistema u krvi iskvavljenih pacova. Tretman PHZom prouzrokovao je više koncentracije ROSa i akumulaciju oksidovanog glutationa u plazmi, dok je glutationski sistem pokazao efikasan antioksidativni kapacitet u RBCu pacova. Kada se uporede dve anemične grupe, izraženiji oksidacioni stres se javlja u plazmi pacova tretiranih PHZom.Projekat ministarstva br. 143035

Glutathione status in the blood of rats after reticulocytosis induced by phenylhydrazine and bleeding

In this experiment, we compared the in vivo effects of phenylhydrazine (PHZ) and bleeding treatment on the redox status and glutathione antioxidative mechanism parameters in the plasma and red blood cells (RBC) of rats. Results showed a lower level of reactive oxygen species (ROS), a higher level of lipid peroxidation and the effective antioxidative role of the glutathione system in the blood of bleeding rats. PHZ-treatment induced higher concentrations of ROS and an accumulation of oxidized glutathione in the plasma, while the glutathione system showed a satisfactory antioxidative capacity in the RBC of rats. When comparing the two anemic groups, the PHZ-treated rats showed marked oxidative stress in the plasma.Cilj ovog rada je bio da se isprate in vivo efekti fenilhidrazinom (PHZ) i krvarenjem indukovane retikulocitoze na parametre redoks i glutationskog antioksidativnog statusa u plazmi i crvenim krvnim ćelijama (RBC) pacova. Rezultati pokazuju niži nivo reaktivnih vrsta kiseonika (ROS), viši nivo lipidne peroksidacije i efikasnu antioksidacionu ulogu glutationskog sistema u krvi iskvavljenih pacova. Tretman PHZom prouzrokovao je više koncentracije ROSa i akumulaciju oksidovanog glutationa u plazmi, dok je glutationski sistem pokazao efikasan antioksidativni kapacitet u RBCu pacova. Kada se uporede dve anemične grupe, izraženiji oksidacioni stres se javlja u plazmi pacova tretiranih PHZom.Projekat ministarstva br. 143035

Glutathione status in the blood of rats after reticulocytosis induced by phenylhydrazine and bleeding

In this experiment, we compared the in vivo effects of phenylhydrazine (PHZ) and bleeding treatment on the redox status and glutathione antioxidative mechanism parameters in the plasma and red blood cells (RBC) of rats. Results showed a lower level of reactive oxygen species (ROS), a higher level of lipid peroxidation and the effective antioxidative role of the glutathione system in the blood of bleeding rats. PHZ-treatment induced higher concentrations of ROS and an accumulation of oxidized glutathione in the plasma, while the glutathione system showed a satisfactory antioxidative capacity in the RBC of rats. When comparing the two anemic groups, the PHZ-treated rats showed marked oxidative stress in the plasma.

Chemical composition, cytotoxic and antioxidative activities of ethanolic extracts of propolis on HCT-116 cell line

BACKGROUND Propolis is a complex resinous sticky substance that honeybees collect from buds and exudates of various plants. Owing to its versatile biological and pharmacological activities, propolis is widely used in medicines, cosmetics and foods. The aim of this study was to evaluate the cytotoxic and antioxidative effects of various ethanolic extracts of propolis (EEPs) on human colon cancer cell line HCT-116 and compare them with their composition determined by HPLC-DAD. RESULTS The most abundant flavonoids in all samples were chrysin, pinocembrin and galangin (12.697-40.811 mu gmg(-1)), while the main phenolic acids were caffeic acid, ferulic acid and isoferulic acid. Dose- and time-dependent inhibition of growth of HCT-116 cells was observed for all propolis samples, with IC50 values ranging from 26.33 to 143.09 mu gmL(-1). Differences in cytotoxic activity of propolis samples were associated with differences in their composition. All EEP samples reduced both superoxide anion radical and nitrite levels and also had strong DPPH-scavenging activity. CONCLUSION All tested propolis samples had pronounced cytotoxic and antioxidative activities.Peer-reviewed manuscript: [http://cherry.chem.bg.ac.rs/handle/123456789/3472

Alteration of oxidative stress parameters in red blood cells of rats after chronic in vivo treatment with cisplatin and selenium

In this study we evaluated the possible protective effects of selenium (Se) on hematological and oxidative stress parameters in rats chronically treated with cisplatin (cisPt). Four groups of Wistar albino rats were examined: a control, untreated rats (I), rats treated with Se (II), rats treated with cisPt (III), and rats treated with Se and cisPt (IV). All animals were treated for 5 days successively and killed 24 h after the last treatment. Hematological and oxidative stress parameters were followed in whole blood and red blood cells (RBC). Results showed that the chronic application of Se was followed by a higher number of reticulocytes and platelets, increased lipid peroxidation and GSH content in the RBC. Cisplatin treatment induced depletion of RBC and platelet numbers and an elevation of the superoxide anion, nitrites and glutathione levels. Se and cisPt co-treatment was followed by an elevation of the hematological parameters and the recovery of the glutathione status when compared to the control and cisPt-treated rats

Effects of acute treatment of vitamin C on redox and antioxidative metabolism in plasma and red blood cells of rats

Ascorbic acid (Vit C) is a reducing agent that efficiently quenches potentially damaging free radicals. The aim of this study was to investigate effects of high acute dose of Vit C on redox and antioxidative status in blood of rats. Our study included two groups of Wistar albino rats: I - untreated group (control); II - group treated with Vit C (500 mg/kg bw, i.p.). In all cases animals were sacrificed after three days of treatment and blood samples were taken at the day of sacrifice. Concentration of Heinz bodies (HB) was determined in whole blood, while the concentration of reactive oxygen and nitrogen species (ROS and RNS), as well as, parameters of antioxidative defense system were followed in plasma and red blood cells (RBC). Our results showed that Vit C treatment induced higher levels of peroxynitrites and lipid peroxidation in plasma. In RBC, Vit C induced higher level of HB, GSSG/2 GSH ratio and glutathione peroxidase activity, while decreased glutathione reductase activity. In conclusion, acute high doses of Vit C act as prooxidant and induced oxidative stress that resulted in lipid peroxidation in plasma and hemoglobin oxidation in RBC. .Projekat ministarstva br. 143035

Effects of acute treatment of vitamin C on redox and antioxidative metabolism in plasma and red blood cells of rats

Ascorbic acid (Vit C) is a reducing agent that efficiently quenches potentially damaging free radicals. The aim of this study was to investigate effects of high acute dose of Vit C on redox and antioxidative status in blood of rats. Our study included two groups of Wistar albino rats: I - untreated group (control); II - group treated with Vit C (500 mg/kg bw, i.p.). In all cases animals were sacrificed after three days of treatment and blood samples were taken at the day of sacrifice. Concentration of Heinz bodies (HB) was determined in whole blood, while the concentration of reactive oxygen and nitrogen species (ROS and RNS), as well as, parameters of antioxidative defense system were followed in plasma and red blood cells (RBC). Our results showed that Vit C treatment induced higher levels of peroxynitrites and lipid peroxidation in plasma. In RBC, Vit C induced higher level of HB, GSSG/2 GSH ratio and glutathione peroxidase activity, while decreased glutathione reductase activity. In conclusion, acute high doses of Vit C act as prooxidant and induced oxidative stress that resulted in lipid peroxidation in plasma and hemoglobin oxidation in RBC. .Projekat ministarstva br. 143035