Clinical Characteristics and Visual Outcomes of Pediatric Optic Neuritis: A Single Center Experience

INTRODUCTION: The aim of this study was to describe the clinical characteristics, visual outcomes of pediatric patients presenting with first-episode of optic neuritis. METHODS: We reviewed medical records of the patients newly diagnosed with optic neuritis younger than 18 years between January 2014 and December 2018 retrospectively. RESULTS: Twenty-eight patients were included to this study. The mean age at first onset of optic neuritis was 13.2+-3.1 years (range 6.2-17.3 years). The mean follow-up period was 4.2+-3.2 (range 0.6-13.08) years. 7 of 28 (25%) patients had recurrent optic neuritis. Optic neuritis involvement was unilateral in 17 of 28 (60%) patients. Forty percent of the patients had idiopathic optic neuritis. Of the six patients with demyelinating lesions in cranial magnetic resonance imaging (MRI) at the first admission, three were diagnosed with multiple sclerosis (MS) at the time of first optic neuritis attack, and three were diagnosed within 13.4+-4.8 months after the first episode. Eight of 21 optic neuritis patients (38%) had oligoclonal band positivity and the incidence of MS was significantly higher in these patients (p=0.014). The mean visual acuity at nadir was 0.48+-0.27 at admission. Whereas it was 0.74+-0.31 and 0.76+-0.33 at 1 and 6 months respectively. There was a strong correlation between first and sixth-month visual acuity (r=0.98, p=0.00). DISCUSSION AND CONCLUSION: Our study demonstrated that poor visual acuity (worse than 0.5) at 1 month can predict poor vision at 6 months. The patients with demyelinating lesions in cranial MRI at their first optic neuritis episode, are more likely to develop MS during the follow-up

A turn off-on fluorescent chemosensor for sequential determination of mercury and biothiols

WOS:000451437100021PubMed:30353352The selective and sensitive determination of biothiols in aqueous media has been great attention due to their important role in biological and pharmacological processes. We synthesized tryptophan functionalized perylene bisimide as a sensing chemosensor for mercury in aqueous solution. This complex between perylene dimide derivate (PDI/Trp) and mercury ions was evaluated and displayed to be turn on fluorescent chemosensor for the determination of biothiols in aqueous media. PDI/Trp showed fluorescence quenching in the presence of Hg2+ and the fluorescence was recovered after addition of biological thiols (cysteine, homocysteine and glutathione). Therefore, PDI/Trp can be employed as a fluorescence probe for the sequencial recognization of Hg2+ and biothiols in aqueous solution

Propylimidazole functionalized coumarin derivative as dual responsive fluorescent chemoprobe for picric acid and Fe3+ recognition: DFT and natural spring water applications

WOS:000778065500001PubMed ID35377047A propylimidazole functionalized coumarin derivative (IPC) was fabricated for the first time and applied as a dual responsive fluorescent chemoprobe for sensitive and selective recognition of picric acid (PA) and Fe3+. Strong fluorescence quenching phenomena of the IPC were observed in H2O/ACN (5/95, v/v) medium (λem=408 nm) upon the additions of Fe3+or PA. The fabricated dual responsive IPC offered good selectivity and sensitivity with the low limit of detection values (0.92 µM for PA and 0.22 µM for Fe3+) lower than the acceptable amounts of Fe3+ and PA by the international official authorities. The validation study for the chemoprobe IPC for PA and Fe3+ was also performed. The interaction phenomena of IPC with PA and Fe3+ based on the findings of a range of experiments were considered and DFT computations were done to verify their recognition mechanisms. The sensing phenomena of IPC towards PA (1:1) and Fe3+ (3:1) were confirmed by the MALDI TOF–MS, FT–IR, 1H–NMR titration and Job's methods. Furthermore, the compound IPC was effectively applied as a fluorescent sensor for Fe3+ and PA detection in real natural spring water samples. Graphical Abstract: [Figure not available: see fulltext.

Fabrication and sensing properties of phenolphthalein based colorimetric and turn–on fluorogenic probe for CO32− detection and its living–cell imaging application

WOS:000656959000066PubMed ID: 33676708Herein, an easy assembled colorimetric and ''turn–on'' fluorescent sensor (probe P4SC) based on phenolphthalein was developed for carbonate ion (CO32−) sensing in a mixture of EtOH/H2O (v/v, 80/20, pH = 7, Britton–Robinson buffer) media. The probe P4SC demonstrated high sensitive and selective monitoring toward CO32− over other competitive anions. Interaction of CO32− with the probe P4SC resulted in a significant increment in emission intensity at λem = 498 nm (λex = 384 nm) due to the strategy of blocking the photo induced electron transfer (PET) mechanism. 1H NMR titration and Job's methods, as well as the theoretical study were carried out to support the probable stoichiometry of the reaction (1:2) between P4SC and CO32−. The binding constant of the probe P4SC with CO32− was calculated as 2.56 × 1010 M−2. The probe P4SC providing rapid response time (~0.5 min) with a satisfactorily low detection limit (14.7 nM) may be useful as a valuable realistic sensor. The imaging studies on the liver cancer cells (HepG2) shows the great potential of the probe P4SC for the sensation of intracellular CO32− anions. Furthermore, the satisfactory recovery and RSD values obtained for water application confirming that the probe P4SC could be applied to sensing of CO32− ion.This work was supported by the Karamanoglu Mehmetbey University with the financial support to use Gaussian-09 and GaussView-5.0.8 software packages (BAP-Grant Numbers: 15-YL-17, 14-YL-20 and 05-M-20)

Chemometric classification and quantification of cold pressed grape seed oil in blends with refined soybean oils using attenuated total reflectance-mid infrared (ATR-MIR) spectroscopy

WOS:000452946300018Of the cold pressed edible oils, grape seed oil (GSO) is one of the highest quality and, consequently the most expensive oil. Because of that, adulteration by mixing GSOs up with lower price edible oils is frequently seen. The classification and determination of adulterant concentration for GSO is hence a focus of great interest. The aim of this study was to investigate the feasibility of ATR MIR spectroscopy coupled with the multivariate methodology for qualitative and quantitative analyses of cold pressed GSO adulteration with refined soybean oil (SBO), by using characteristic wavenumber regions. Thirty three pure oils and ninety six blends were analyzed using ATR MIR spectroscopy in combination with PCA, WA, SIMCA and PLSR analysis. SIMCA models provided an excellent classification for pure GSO and other samples. The classification limits by ATR MIR spectroscopy was also below 5%. Quantitative analyses were performed by minimization of RMSE of cross-validation values, which resulted in excellent predictions (R-2 > 0.99). The prediction parameters for the sample sets were: the RMSEC were in the range 0.59-2.09% and, RMSECV were in the range 0.92-5.60%, hence on the basis of the PLSR models, quantification of adulterant could be determined at levels < 0.59%.Scientific Research Project Center of Karamanoglu Mehmetbey University [18-M-18]; Scientific Research Project Center of Ankara University; TUBITAKTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [TUBITAK-116Z159]; TUBITAK under the 2219-Research Fellowship ProgramThis study is supported financially by the Scientific Research Project Center of Karamanoglu Mehmetbey University (Project number 18-M-18). The authors would like to thank the Scientific Research Project Center of Ankara University and TUBITAK (Project number TUBITAK-116Z159) for providing the financial support to gain Unscrambler (R) X10.4 (CAMO software, Oslo, Norway) program. The authors would also like to thank TUBITAK under the 2219-Research Fellowship Program for providing the financial support to carry out this research work

An ultrasensitive ″OFF–ON″ fluorogenic sensor based on thiazole derivative for Zn2+: Food supplement, water and bio–imaging applications

WOS:000687587600002In the present study, the design of a novel turn–on fluorescent probe ((E)–5–methoxy–4–((thiazol–2–ylimino)methyl)benzene–1,3–diol) (TBD) was reported. TBD showed an excellent fluorogenic response (λex = 420 nm, λem = 530 nm) to sense Zn2+ in HEPES buffer/MeCN (5/95, v/v; pH = 7.4) media. The quantum (Φ) yield of the probe TBD increased importantly with Zn2+ and was calculated for TBD (Φ = 0.073) and TBD–Zn2+ complex (Φ = 0.78). The LOD and binding constant (Ka) of TBD were 1.29 nM and 1.102 × 106 M−1, respectively. The sensing process of TBD toward Zn2+ (1:1) was verified by the Job's plot, MALDI–TOF–MS, and 1H NMR titration studies. The ''off–on'' fluorogenic response of TBD was qualified to the photo–induced electron transfer (PET) process. The experimental outcomes were also verified by theoretical DFT studies. Furthermore, TBD had been successfully utilized to detect Zn2+ in food supplement products, drinkable water samples and living–cells for on–site analysis

Coumarin based highly selective "off-on-off" type novel fluorescent sensor for Cu2+ and S2- in aqueous solution

WOS:000395070900008PubMed:27995460Solvent free synthesis of 6,7-dihydroxy-3-(3-chlorophenyl) coumarin (CFHC) was designed and obtained by the interaction of 2-(2,4,5-trimethoxyphenyl)-1-(3-chlorophenyl)acrylonitrile with pyridinium hydrochloride in the presence of silica gel by using microwave irradiation. The characterization of CFHC was confirmed by FT-IR, H-1, C-13, C-13-APT and 2D HETCOR spectroscopy methods. The optical behavior of CFHC towards metal ions was investigated by UV-visible and fluorescence spectroscopy. CFHC showed "on-off" type fluorescence response towards Cu2+ with high selectivity in aqueous solution (CH3CN/H2O, 9/1, v/v). Once binding with Cu2+, CFHC-Cu2+ complex also displayed high selectivity for sulfide, resulting in "off-on" type sensing of sulfide anion.TUBITAKTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [110 T652]The authors are grateful to the Research Fund of the TUBITAK for their support (for the synthesis of compound) with the project No - 110 T652