4 research outputs found
Double-application of platelet-rich plasma on bone healing in rabbits
Objective: Platelet-rich plasma (PRP) is considered to enhance bone formation especially at early stages of wound healing, depending on the limited and short life-span of platelets and growth factors. The aim of this study was to evaluate efficacy of double-application of PRP (DA-PRP) on bone healing in a rabbit calvarial defect model. Study design: Twenty-eight rabbits, each had two surgically prepared calvarial bone defects (10mm diameter), were included in this study and randomly divided into six groups. Defects (n=56) were treated with single-application of PRP (SA-PRP)(n=10), SA-PRP and beta-tricalciumphosphate (SA-PRP+TCP)(n=10), DA-PRP (n=8), DA-PRP and beta-tricalciumphosphate (DA-PRP+TCP)(n=8), beta-tricalciumphosphate (TCP)(n=10) or left empty (Control)(n=10). Animals were sacrificed at 30 days postoperatively. Results: The new bone (NB%) and defect fill (DF%) percentages were calculated from histological slides by image-analyzer software and statistically analysed. All test groups showed higher NB% than control, but differences among all groups were insignificant. The TCP treated groups had significantly higher DF% than groups treated without TCP, however the DF% differences between control, SA-PRP and DA-PRP or TCP, SA-PRP+TCP or DA-PRP+TCP were insignificant. Conclusion: Although new bone formation was histomorphologically remarkable at double-application PRP groups, statistical analyses of the histomorphometric data revealed no significant difference. © Medicina Oral S. L
EVALUATION OF GINGIVAL CREVICULAR FLUID INTERLEUKIN-8 LEVELS OF PATIENTS WITH DOWN S SYNDROME BEFORE AND AFTER PERIODONTAL THERAPY
DSİ u 21. kromozomun trisomisinin neden olduğu genetik bir
bozukluktur. DSİ unun en büyük özelliği çocuklardaki zeka geriliğidir. DSİ
lu bireylerde periodontal hastalõk prevalans ve şiddetinin yüksek olduğu
bilinmektedir. Savunma sistemiyle ilgili yapõlan çalõşmalar sonucunda
DSİnda esas immün defektin timus bağõmlõ sistemde olduğu öne
sürülmüştür. DSİ lu bireylerde özellikle nötrofil kemotaksisinde azalma ve
fagositoz ile karakterize bozukluklar olduğu bilinmektedir. Bir sitokin olan
IL-8\' in nötrofiller için kemotaktik olduğu ve bu hücrelerin endoteliyal
hücrelere yapõşmalarõnõ arttõrdõğõ öne sürülmüştür. Bu çalõşmada, DSİ lu
bireylerin DOS\' nda nötrofillerin kemotaksisinde önemli rolü olduğu
düşünülen IL-8 seviyelerinin periodontal tedavi öncesi ve sonrasõnda ve
aynõ yaş grubundaki sistemik ve periodontal olarak sağlõklõ kontrollerle
karşõlaştõrõlmasõ ve aynõ zamanda DSİ lu bireyler için özel olarak
hazõrlanan ve görsel etkinliği fazla olan bir programla oral hijyen eğitimi
(OHE) vermek ve bu eğitim içinde ebeveynleri de dahil ederek verilecek
periodontal tedavinin etkinliğini uzun dönemde değerlendirmek
amaçlanmıştır.
Çalõşmada DSİ lu 15 birey, ebeveynleri ve sistemik ve
periodontal yönden sağlıklı 15 birey yer almıştır. Tedavi öncesinde
periodontal sağlık durumu tüm gruplarda PI, GI, CD ve radyografik
yöntemler (panoramik) gibi klinik değerlendirmelerle belirlendi. Daha sonra
tüm ebeveynlere ve DSİ lu bireylere bireysel olarak OHE, diştaşõ temizliği
ve kök yüzeyi düzleştirmesi ve kazõmasõnõ (KDK) içeren başlangõç
periodontal tedavi verildi. Periodontal tedavi sonrasõ 1., 3. ve 6. aylarda
klinik indeksler tekrarlandõ ve düzenli olarak OHE ve periodontal tedavi
verildi. Başlangõç klinik indekslerin alõnmasõndan 1 gün sonra DOS
toplandõ. Faz I periodontal tedavi sonrasõ 1. ayda tekrar DOS örnekleri
toplandõ. DOS\' daki IL-8 seviyeleri için analizler ELİSA yöntemiyle
değerlendirildi.
Tüm gruplarda Faz Ι periodontal tedavi ve özel olarak verilen
OHE\' ni takiben başlangõç seviyeleriyle kõyaslandõğõnda PI, GI ve CD
değerlerinde 1, 3 ve 6. aylarda anlamlõ bir düşüş gözlenmiştir. DOS IL-8
seviyeleri DSi lu bireylerde kontrol grubuna göre anlamlõ bir farklõlõk
göstermemiştir. Aynõ zamanda, DS lu bireylerin TÖ (B) ve TS (1.ay)
değerleri karşılaştırıldığında anlamlı bir farklõlõk gözlenmemiştir.
Çalışmamızda elde ettiğimiz sonuçlar, DOS\' daki IL-8
miktarının DSİ lu bireylerdeki periodontal durumla ilişkili olmadığını öne
sürmektedir. Bununla birlikte elde ettiğimiz sonuçlar DSİ lu bireylere ve
ebeveynlerine düzenli olarak verilen OHE\' nin ve periodik periodontal
tedavinin DSİ lu bireylerdeki periodontal hastalõğõn ilerlemesinin
durdurulmasõnda etkili olabileceğini göstermektedir.DS is an autosomal disorder caused by an extra
chromosome 21. The syndrome is characterized by mental deficiency in
these children. The high prevalance and severity of periodontal disease
has been reported in subjects with DS patients. The high prevalence of
periodontal disaese in these patients may be because of impaired host
defense associated with reduced neutrophil chemotaxis, impaired
neutrophil phagocytoxis, reduced T lymphocyte counts and immature T
lymphocytes. IL-8 is a proinflammatory chemokine, a potent
chemoattractant and activator of neutrophils. IL-8 is is produced in the
epithelium of the gingival tissues in response to bacterial exposure. The
aim of this study was to evaluate the effects of phase I periodontal
treatment on gingival crevicular fluid (GCF) levels of IL-8.
15 DS, their parents and 15 periodontally healthy controls
(C) were included in this study. Plaque index (PI), gingival index (GI)
probing depth (PD) measurements were recorded a single examiner and
radiographic examinations were performed from both DS patients and
primer care givers. After clinical and radiographical examinations GCF
samples were collected from the DS and C patients before treatment (BT).
DS patient s and their parent s oral health was evaluated over the
following 6 months which period monthly care consisted of professional
tooth cleaning, scaling and root planing, oral hygiene instructions and
counselling to the primer care givers. Clinical parameters were recorded at
the baseline and the 1st,3rd and 6th months of the study. GCF samples
were collected from the DS and C patients once more 1 month after the
therapy (AT ) Assays for IL-8 were performed by an ELISA method.
DS and parent groups showed statistically significant
reductions in PI, GI and PD scores following the phase I periodontal
treatment. PI, GI and PD scores at the 1st,3rd and 6th months exhibited a
statistically significant decrease when compared to the baseline. GCF
levels of IL-8 were significantly higher in DS group before the treatment
than controls. But no significant difference was obtained when GCF BT
and AT IL-8 levels of DS patients and DS groups BT and control groups
IL-8 levels were compared.
These results suggest that IL-8 can t be associated with the
periodontal status in DS patients and periodic preventive care and
systematic oral hygiene instructions given to the DS patients and their
primer care givers is effective for suppressing the progression of
periodontal disease in patients with DS