The role of oxidative stress genes and effect of pH on methylene blue sensitized photooxidation of Escherichia coli

In this study, the survival time of wild type E. coli W3110 and 11 mutants was analysed with a plate count method in methylene blue added or control groups under daylight fluoroscence illumination (4950 lux) at different pH values (5.0, 6.0, 7.0, and 8.0) in phosphate buffer. As a result, while the number of bacteria did not decrease under photooxidative stress at pH 5.0 and 6.0 during a 6-hour incubation, the wild type and all mutants decreased more than 2 log. at pH 8.0, and approximately one log. at pH 7.0. It was determined that a 2 log decrease in wild type E. coli takes 3.7 h according to t99 value at pH 8, these values were 2.39 h in the katE mutant, 2.64 h in the soxR mutant, 2.67 h in the oxyR mutant, 2.71 h in the sodB mutant, 3 h in the btuE mutant, 3.38 h in the zwf mutant and 3.40 h in the soxS mutant, respectively (p < 0.05). The roles of these genes were proved with complement tests. Finally, it is found that the effectiveness of photooxidative stress is in direct relation with pH, and the katE, soxR, oxyR, sodB, btuE, zwf, and soxS genes are important for the protection against this stress

Batı Karadeniz Bölgesinde anormal servikal sitoloji risk faktörleri ve sağlık sigortasının önemi

Objective: To evaluate the prevalence of abnormal cervical cytological findings in the Western Black Sea Region and investigate an association between socio-demographic risk factors and the presence of cytological abnormalities. Material and Method: The reports of 11,539 cervical smears diagnosed according to Bethesda System 2001 version in the Pathology Department between January 2011 and December 2012 were reviewed retrospectively from the hospital records and cytopathology reports. Repeated smear results, unsatisfactory smear results, patients with known gynecologic malignancy history, smear results of patients with hysterectomy and smear results of patients whose socio-demographic information could not be obtained were excluded from the evaluation. The results of 7,740 patients who met the criteria for the study were evaluated. Results: The prevalence of cervical cytological abnormalities was 1.8 % in general. The prevalence rates for atypical squamous cells with undetermined significance (ASC-US), atypical squamous cells, cannot exclude a high-grade squamous intraepithelial lesion (ASC-H), low-grade squamous intraepithelial lesions (LSIL), high-grade squamous intraepithelial lesions (HSIL), and atypical glandular cells (AGC) were 1.16%, 0.11%, 0.29%, 0.15%, and 0.03% respectively. The prevalence of cytologically diagnosed cervical invasive neoplasia was 0.025%. Advanced age, low education level (primary school or less) and not having health insurance were found as to be risk factors for preinvasive and invasive lesions. Women who had a high school education and previously had a smear test had decreased risk for developing preinvasive and invasive lesions. Conclusion: This study shows prevalence of abnormal cervical cytology findings and associoted risk factors in the Western Black Sea Region of Turkey. The most important risk factor was identified as not having health insurance

Serum Zinc Levels in Patients with Acne Vulgaris and Its Correlation with Severity of Acne

Objective: Acne vulgaris the most common cutaneous disorder affecting adolescent and young adults. Zinc is an essential element for human. We aimed to evaluate the serum zinc level in patients with acne vulgaris and correlate with the severity of disease. Methods: The study included 43 patients with acne vul­garis and 37 healthy control subjects. The severity of acne was assessed according to Global Acne Grading System. The serum zinc levels were measured both patients and control subjects. Results: The serum zinc level in acne patients were 81.48 ± 14.21 μg/dl and 83.69 ± 12.43 μg/dl in control subjects. There was no statistically significant difference between patients and control subjects (p=0.46).There was a negative correlation between acne severity and serum zinc levels but there was no statistically significant (r=-0.024, p=0.88). Conclusion: The results of our study revealed no relation between serum zinc levels and acne. Further studies are needed

Minimally Invasive Approach for Combined Cholecystectomy and Total Hysterectomy with LigaSure

Wide applications of operative laparoscopy can be carried out as combined procedures for multipl pathologies. In single time anesthesia, these combined minimally invasive procedures gives the advantages of lesser pain and morbidity, faster recovery and shorter hospital stay. We report a 58 years old woman treated for uterine myomas and cholelithiasis. The patient underwent laparoscopic total hysterectomy, bilateral salpingo-oopherectomy and laparoscopic cholecystectomy in single session. Procedures were performed with cross-specialty cooperation between a gynecologist and a general surgeon and were completed laparoscopically without intraoperative or postoperative complications. And the patient was discharged 24 hours after the operation

Combined procedure of cesarean delivery and preperitoneal mesh repair for inguinal hernia: An initial experience

Background: Combined surgery for cesarean delivery and preperitoneal mesh repair for inguinal hernia has not been previously reported. Objectives: Our aim was to describe the method and to present the results of this simultaneous surgery through a single incision. Methods: From 2012 to 2014, 15 patients underwent cesarean delivery combined with preperitoneal mesh repair for inguinal hernia. All patient characteristics and perioperative findings were recorded. Results: Among 15 patients, 13 had unilateral inguinal hernias and two had bilateral hernias. The mean times spent for unilateral and bilateral hernias were 35.8 minutes (range, 30–45 minutes) and 67.5 minutes (range, 65–70 minutes), respectively. Direct and indirect hernias were present in one and 15 patients, respectively. One patient had mixed hernia. No significant complication was observed perioperatively. Hospital stay ranged from 1 day to 3 days (mean, 1.87 days), and all patients were discharged without any problem. No recurrence was found during the follow-up periods. Conclusion: Single anesthesia, single incisional scar, and single hospitalization are the major advantages of this simultaneous approach of cesarean delivery and preperitoneal mesh repair for inguinal hernia. Our analysis suggests that this combined procedure can be performed safely in selected cases

Laparoscopic Sacrocolpopexy or Sacrohysteropexy with or Without Burch Colposuspension: The Results of 36 Patients in Our Clinic

OBJECTIVE: Our aim was to investigate the results and the effectivity of laparoscopic sacrocolpopexy or sacrohisteropexy with or without Burch colposuspension in pelvic organ prolapse with or wihout urinary incontinence patients in our clinic. MATERIAL AND METHOD: From January 2011 to January 2015 we performed laparoscopic sacrocolpopexy or sacrohysteropexy with or without Burch colposuspension for pelvic organ prolapse in 36 selected patients. Demographic and clinical data, intraoperative findings and postoperative course were recorded. POP-Q examination was used for pelvic prolapsed patients. Burch colposuspension was added to patients if they had stress urinary incontinence proven with physically examination and urodinamic tests. Twenty two patients underwent laparoscopic sacrocolpopexy or sacrohysteropexy and 14 patients underwent laparoscopic sacrocolpopexy or sacrohysteropexy with Burch colposuspension. RESULTS: Laparoscopic sacrocolpopexy without Burch colposuspension (Group 1) (n=22) group’s mean operation time and the mean blood loss were 51.1±11.8 minutes (range: 40-85 min.) and 39.3±13.9 cc (range: 30-70 cc) respectively. Laparoscopic sacrocolpopexy with Burch colposuspension (Group 2) (n=14) group’s mean operation time and the mean blood loss were 88.5±13.5 minutes (range: 85-100 min.) and 65.0±11.0 (range: 60-80 cc) respectively (for mean operation time p=<0.001, for mean blood loss p=<0.001). Nine of the patients had operations with uterus preservation. All patients were discharged the following day. In Group 2 two patients developed denovo detrusor instability and improved with the administration of oxybutynin. In group 1 postoperatively, 8 patients developed denovo urinary incontinence. In the treatment of this condition we have added two patients Burch colposuspension operations, 6 patients underwent transobturatuar tape TOT. Two patients did not want to have reoperations, they received medical treatment. CONCLUSION: Laparoscopic sacrohysteropexy or sacrocolpopexy with Burch colposuspension offer minimally invasive approaches and excellent definitive treatment option for patients with pelvic organ prolapse and urinary incontinence with long-term success rates ranging from 93-99%. In our study, our data shows that laparoscopic approaches for treatment of pelvic organ prolapse and urinary incontinence effective in hysterectomized or non-hysterectomized patients. Long-term prospective studies may provide additional useful data for these procedures

Minimally Invasive Approaches for Combined Gynecologic Surgeries: Our Three Years Clinical Experiences

OBJECTIVE: To evaluate outcomes of gynecological patients with multiple pathologic conditions who were treated with combined laparoscopic procedures. MATERIAL AND METHOD: We retrospectively analyzed 48 consecutive patients who underwent a combination of laparoscopic surgical procedures during a single operative session in Turgut Ozal University Hospital, Department of Gynecology. Outcomes included operative time, intraoperative blood loss, hemoglobin levels, mean hospital stay and complication rate. To investigate the relationship between body mass index (BMI) and other variables, we assigned each patient to three BMI groups. RESULTS: The most common combination of procedures was laparoscopic hysterectomy and laparoscopic Burch colposuspension (18 patients). Blood loss was positively correlated with number of ports. Higher BMI was correlated with greater blood loss and operative time but not with complication rate. CONCLUSIONS: Multiple pathologic conditions could be treated safely and effectively in a single laparoscopic operation. Obesity did not appear to be a risk factor for complications

Ovarian Fibroma as a Possible Cause of Fever of Unknown Origin

Fibromas are the most common benign solid tumors of the ovary. Clinically, they are asymptomatic and preoperative diagnosis is often difficult. In this paper we report a case of fibroma as a cause of fever of unknown origin (FUO). A 26-year-old woman referred to our hospital with a one-month history of intermittent fever. Ultrasonography as well as pelvic computed tomography scans showed a hypoechoic appearance in the right adnexal region which was diagnosed as pyosalpinx, in the light of clinical setting. Pus drainage was then planned but 3cc of serous material was drained from the adnexal mass with ultrasound-guide. Despite antibiotic therapy, her fever continued. She had a comprehensive work-up for FUO, including rheumatologic, infectious, and malignant etiologies. Magnetic resonance imaging (MRI) scans of the pelvis showed two well defined ovarian lesions, which were suggestive of an ovarian fibroma. MRI findings that are suggestive of gynecological infectious diseases were not detected. Then laparoscopy was scheduled. The patient responded well to surgery and the fever resolved after surgery. She is now in the ninth postoperative month and still has no fever

An Unusual Side Effect of Etonogestrel Implant: Facial Paralysis

Etonogestrel single rod subdermal implant provides an effective contraceptive protection for up to 3 years with no reported pregnancies in clinical trials Unfortunately, similar to other long-term progestinonly contraceptives, Implanon® leads to irregular uterine bleeding in the majority of users. Bleeding disturbances are the main reason for discontinuation of therapy. Other reported nonmenstrual adverse effects of Implanon® include headache, acne, weight gain, and mastalgia. In this paper we do report a case of facial paralysis during the use of Implanon®. A 35-year-old woman with an Implanon® contraceptive device in situ presented with amenore. The implant had been inserted 4 years previously which was changed one year before the removal. Because of the patient being amenorrhoeic for one year, the Implanon® was removed in January 2010. A few months later after the removal of the implant she reported an improvement of the facial paralysis that had started 4 months after the second insertion of Implanon®