39 research outputs found
Kuzeydoğu anadolu bölgesinde ortaya çıkan koyun ve keçi çiçeği olgularının serolojik, virolojik ve epizootiyolojik yönden incelenmesi / Virological, serological and epizootiological investigations of sheep pox and goat pox diseases in northeast anatolian region, Turkey
Kuzeydoğu anadolu bölgesinde ortaya çıkan koyun ve keçi çiçeği olgularının serolojik, virolojik ve epizootiyolojik yönden incelenmesi / Virological, serological and epizootiological investigations of sheep pox and goat pox diseases in northeast anatolian region, Turkey
Koyun-keçi çiçeği hastalığının polimeraz zincir reaksiyonu ile tanısında farklı klinik örneklerin kullanım
Elazığ Malatya ve Tunceli İllerinde Koyunlarda Görülen Abort Vakalarında Pestivirusların Rolünün Araştırılması
Detection of bovine torovirus in fecal specimens from calves with diarrhea in Turkey
Bovine torovirus (BToV), a member of the family Coronaviridae, is an established gastrointestinal infectious agent in cattle. In this study, we performed a survey to detect BToV in Turkey between 2009 and 2011 using 235 fecal samples from neonatal calves with diarrhea that were analyzed by the nested reverse transcription (RT) PCR method using primers located in the consensus sequences of the BToV membrane (M) gene. The BToV M gene was detected in 4.7 % (11/235) of the samples using the nested RT-PCR method. The nucleotide sequences of partial M fragments from the BToV isolates, including the newly identified Turkish isolates, showed more than 96 % identity. The result indicates that BToV is one of the pathogens that contribute to neonatal calf diarrhea cases in Turkey
Molecular characterization of the bovine noroviruses from diarrheic calves in Turkey
In this study, we performed a survey to detect bovine noroviruses (BNoVs) in Turkey between 2009 and 2011 using 235 fecal samples from neonatal calves with diarrhea that were analyzed by nested reverse-transcription RT-PCR, using primers located in the consensus sequences of the BNoV RNA-dependent RNA polymerase (RdRp) gene. The BNoV RdRp gene was detected in 1.7% (4/235) of samples by nested RT-PCR. The nucleotide sequences of partial RdRp fragments from the BNoV isolates including the newly identified Turkish isolates showed more than 86.3% nt and 96.8% aa identity with each other. Interestingly, in a comparison of the nt and aa sequences, one of the Turkish BNoV strains was found to be 100% aa identical with some Italian and Tunisian strains. In conclusion, BNoV is one of the pathogens that contributes to neonatal calf diarrhea cases in Turkey