Acute leukemia of childhood: A single institution's experience

The aim of this study was to investigate distribution of immunophenotypic and cytogenetic features of childhood acute leukemia (AL) in the cohort of 239 newly diagnosed patients registered at the leading pediatric oncohematology center in the country during a six-year period (1996-2002). With approximately 60-70% of all childhood AL cases in Serbia and Montenegro being diagnosed and treated in this institution the used data represent a valid research sample to draw conclusions for entire country. On the basis of five phenotypic markers, the distribution of immunological subtypes was as follows: 169 (70.7%) expressed B-cell marker CD19 (137 were CD10 positive and 32 CD10 negative), 37 (15.5%) belonged to T-lineage acute lymphoblastic leukemia (T-ALL) (cyCD3 positive), and 33 (13.8%) were acute myeloblastic leukemia (AML) (CD13 positive and/or CD33 positive in the absence of lymphoid-associated antigens). The ratio of males and females was 1.5:1. Most of the cases were between the ages of 2 and 4, and were predominantly B-lineage acute lymphoblastic leukemia (B-ALL) cases. Another peak of age distribution was observed at the age of 7. The frequency of T-ALL (18% of ALL) was similar to that reported for Mediterranean countries: France (19.4%), Greece (28.1%), Southern Italy (28.3%), and Bulgaria (28.0%). Cytogenetic analyses were performed in 193 patients: 164 ALL and 29 AML. Normal karyotype was found in 57% of ALL and in 55% of AML patients, while cytogenetic abnormalities including structural, numerical, and complex chromosomal rearrangements were found in 43% of ALL and in 45% of AML patients. Our results represent a contribution to epidemiological aspects of childhood leukemia studies

The toxicity of isoproterenol to myocardial tissue in experimental conditions

Ispitivan je utjecaj jednokratne aplikacije izoproterenola (ISP) na ukupnu aktivnost i izoenzimsku sliku kreatin kinaze (CK) i laktat dehidrogenaze (LDH) u serumu štakora te korelacija tih promjena sa patohistološkim promjenama u tkivu miokarda. lzoproterenol, apliciran u dozi od 25 mg/100 g tjelesne mase, uzrokovao je statistički značajan porast ukupne aktivnosti CK i LOH u serumu te aktivnosti kardiospecifičnih izoenzima CK-MB, LDH-1 i LDH-2 u vremenu od šest sati nakon aplikacije izoproterenola. Također je nađena izomorfna izoenzimska slika LDH što govori u prilog nastanka kardiogenog šoka. Uz navedene opisane promjene nađen je i porast aktivnosti izoenzima CK-MM i LDH-5, a što se znade tumačiti kao sekundarna pojava kardiogenog šoka i posljedičnog oštećenja tkiva sa anaerobnim tipom metabolizma, kao što je jetra. Prethodni nalazi potvrđeni su patohistološkom analizom tkiva miokarda gdje je nađena koagulacijska nekroza sa miocitolizom te undulacije mišićnih stanica srca kao znaka kardiogenog šoka.The effect of toxic doses of isoproterenol (ISP) on total activities and isoenzyme patterns of creatine kinase (CK) and lactate dehydrogenase (LOH) in rat sera was investigated and correlated with histopathological changes in the myocardial tissue. A single dose of 25 mg ISP per 100 g body weight caused a statistically important elevation of CK and LOH total activities and of the activities of cardiospecific isoenzymes CK-MB, LOH-1 and LOH-2 six hours after intraperitoneal administration. The isomorphic LOH isoenzyme pattern, which was also observed, was taken to be a proof of the ISP-induced cardiogenic shock. The increase in the activity of CK-MM and LOH-5 isoenzymes could be explained as a secondary consequence of cardiogenic shock and of the consecutive damage of the tissue with anaerobic metabolism such as liver. The findings were confirmed by a histopathological analysis showing the development of coagulative necrosis and myocytolysis as well as undulations of heart muscle cells as a sign of cardiogenic shock

Influence of Ultrasonic and Microwave Irradiation on Cation Exchange Properties of ClayMaterial

This study deals with optimization of the clay activation process using artificial neural network models and multi-objective optimization function. Different artificial neural network models were used for description of the relation between clay sorption capacity and the activation treatment process (power and time of clay exposure to ultrasonic and/or microwave irradiation). Two methodologies (feed-forward and cascade-forward) in combination with five different training algorithms (random order incremental training with learning functions, resilient backpropagation, one-step secant backpropagation, Levenberg-Marquardt backpropagation, Bayesian regularization backpropagation) were applied in order to obtain an optimal artificial neural network model. The optimal artificial neural network model showed good predictive ability (relative error 6.02 % based on external validation data set). In-house developed multi-objective criteria function was used in combination with the developed artificial neural network model and calculated optimal activation was determined (5 minutes of ultrason

Projekat opšteg kaznenog zakonika za Kraljevinu Norvešku : Motivi izraćeni od komisije, postavljene Kraljevskom Odlukom od 14 novembra 1885 god. po želji Ministarstva pravde nemačkog carstva /

Mode of access: Internet

The effect of cannabinoid receptor 1 blockade on hepatic free fatty acid profile in mice with nonalcoholic fatty liver disease

We used rimonabant to investigate the role of CB1 receptor on hepatic FFAs profile during NAFLD. Male mice C57BL/6 were divided into: control group fed with control diet 20 weeks (C; n = 6); group fed with HFD 20 weeks (HF; n = 6); group fed with control diet and treated with rimonabant after 18 weeks (R; n = 9); group fed with HFD and treated with rimonabant after 18 weeks (HFR; n = 10). Rimonabant (10 mg/kg) was administered daily to HFR and R group by oral gavage. Rimonabant decreased liver palmitic acid proportion in HFR group compared to HF group (p  lt  0.05). Liver stearic and oleic acid proportions were decreased in R group compared to control (p  lt  0.01 respectively). Rimonabant increased liver linoleic and arachidonic acid proportions in HFR group compared to HF group (p  lt  0.01 respectively). CB1 blockade may be useful in the treatment of HFD-induced NAFLD due to modulation of plasma lipid and hepatic FFA profile

Partitioning of particulate matter and elements of suburban continental aerosols between fine and coarse modes

The results presented in this work demonstrate for the first time a distribution of elements in the spectral analysis of aerosols in the suburban continental Balkan Peninsula. Samples were collected in the suburban area of Belgrade (Serbia) in the period from March 2012 till December 2013. Results presented here are from long-term measurements of masses of size-segregated aerosols and macro- and microelements in the range of PM0.27–16. The following elements were analyzed: Al, Ag, As, Ba, Ca, Cd, Co, Cr, Cu, Fe, K, Mg, Mn, Mo, Na, Ni, Pb, Sb, Ti, Tl, V, and Zn; levels of Be, Hg, and Se were under the detection limits in all samples. Average concentrations and time and seasonal variations of particulate matter (PM) as well as element contents and their percentage shares are given. The results showed the domination of particle content around the accumulation mode in the range of 0.53 < Dp < 1.06 μm, but the fractional distribution of elements showed maximal average concentrations in different fractions depending on the origin of each element. Crustal elements (Al, Ca, Fe, Mg, Mn, Ti, etc.) dominated in coarse mode, while anthropogenic elements (As, Cd, Cu, Pb, Sb, etc.) were mainly distributed in fine mode fractions. Some elements, such are As and Ni, were detected in investigated aerosols only occasionally, while others, such as Ca, Fe, and Mg, were detected in all analyzed samples. The application of multivariate analysis (PCA) demonstrated the connection between the elements of similar origin, in fine fractions mainly of anthropogenic origin, while in coarse mode of crustal origin, indicating the resuspension with contribution of about 40%. The contents of some measured elements were compared with their contents in aerosols in some European suburban areas