Effect of Exercise on Oxidant and Antioxidant Systems in Rat Kidney Tissue with Hyperthyroidism

Oxidative stress is involved in the pathogenesis of many diseases. Hyperthyroidism is associated with increased oxidative tissue damage. The aim of this study is to investigate whether if there is any protective effect of a regular endurance exercise on oxidative stress in kidney tissues of rats with experimental hyperthyroidism. Twenty-three male Spraque Dawley rats were divided into four groups: Control, hyperthyroid, exercise and, hyperthyroid with exercise. Hyperthyroid was induced in rats by subcutaneous injections at a dosage of 250 µg L-thyroxine per kg body weight and hyperthyroidism was confirmed by the measurements of TSH, FT3 and FT4 in serum. Endurance training lasted 8 weeks and consisted of 45 minutes daily treadmill running at a speed of 23 m/minute for 5 days a week. The levels of MDA, GSH and, GSH-Px activity were measured in kidney homogenates. MDA level of the hyperthyroid group was significantly higher than those of the control group(p=0.022). On the other hand, the MDA level of the exercise group was significantly lower than the MDA level of the hyperthyroid group (p=0,001). GSH level and GSH-Px activities were lower in the hyperthyroid group comparing the control group (p<0.05). GSH level and GSH-Px activity in the exercise group were higher than compared with the hyperthyroid group (p<0.05). The results of our study show that L-thyroxine injection may increase oxidative stress by causing lipid peroxidation at kidney tissues and endurance training may decrease oxidative stress. Keywords: Endurance training, glutathione, glutathione peroxidase, hyperthyroidism, kidney tissue, malondialdehyde DOI: 10.7176/JMPB/70-05 Publication date: April 30th 202

Recent Advances in Health Biotechnology During Pandemic

The outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which emerged in 2019, cut the epoch that will make profound fluctuates in the history of the world in social, economic, and scientific fields. Urgent needs in public health have brought with them innovative approaches, including diagnosis, prevention, and treatment. To exceed the coronavirus disease 2019 (COVID-19) pandemic, various scientific authorities in the world have procreated advances in real time polymerase chain reaction (RT-PCR) based diagnostic tests, rapid diagnostic kits, the development of vaccines for immunization, and the purposing pharmaceuticals for treatment. Diagnosis, treatment, and immunization approaches put for- ward by scientific communities are cross-fed from the accrued knowledge of multidisciplinary sciences in health biotechnology. So much so that the pandemic, urgently prioritized in the world, is not only viral infections but also has been the pulsion in the development of novel approaches in many fields such as diagnosis, treatment, translational medicine, virology, mi- crobiology, immunology, functional nano- and bio-materials, bioinformatics, molecular biol- ogy, genetics, tissue engineering, biomedical devices, and artificial intelligence technologies. In this review, the effects of the COVID-19 pandemic on the development of various scientific areas of health biotechnology are discussed

The effects of melatonin administration on vasomotor activity of thoracic aorta in rats with experimental hyperthyroidism

The effects of melatonin administration on vasomotor activity of thoracic aorta in rats with experimental hyperthyroidismAtaturk University: 2017/615

The importance of physical activity in the present and past: opinions of thinkers

Aim: Thephysical activity is getting decrease due to developing of technology, work life and the urbanization, so this situation has a bad role on the human health. The aim of this study is to compare the information that presents the relationship of exercise approaches and exercises in the past period with today's information and evaluate the perspective of the exercise at that time.Methods: The works of scientists and philosophers living in the past period were investigated by examining the opinions of physical activity and body health.Results:Ibn-i Sina who lived in the eleventh-century and made a reputation especially as a physician, was a scientist. He defined exercise as a series of voluntary movements that consist of deep and rapid respiration, Moreover,he thought that regular exercisehad a protective effect against temperament and humoral disturbances. Another well-known thinker, Ibn-i Miskeveyh said "children and adults drop into a habit that moving, riding, making physical exercise, and avoid to acquired habits that are the opposite of them "He emphasized that physical education and sports have a great positive effect on human health.Conclusion: The views of archaic scholars about physical activity has a significant importance for occurring the physical, mental and spiritually healthier societies

Determination of the effects of ın vivo melatonin application on thyroid hormone levels in kidney tissue of experimental hyperthyroid rats

Determination of the effects of ın vivo melatonin application on thyroid hormone levels in kidney tissue of experimental hyperthyroid ratsAtaturk University: TDK-2017-615

Responses of aortic ring vascular smooth muscle in rats with hyperthyroidism to phenylephrine and potassium chloride

Aim: The effects of thyroid hormones on receptor numbers and pharmacological contractile responses of vascular smooth muscle appear to be controversial. The aim of the present study was to evaluate the vascular responses to potassium chloride (KCl) and phenylephrine (PE) in a rat model of hyperthyroidismMethods:The study was approved by the Ethical Committee of The Animal Research of Ataturk University. Wistar Albino type of rats were divided into two groups: Control (n=5) and hyperthyroidism(n=5). Hyperthyroidism was induced by intraperitoneal injection of L-thyroxine (0.3mg/kg/day) for two-weeks. The thoracic aorta rings suspended in an isolated organ bath with Krebs-Henseleit buffer. Maximal tension was measured in response to KCl and PE in rings with intact endothelium. The data were evaluated by one-way ANOVA with post-hoc Tukey test.Results:Our results showed that contractile responses of the aortic rings of with hyperthyroidism to PE (10-7M) were significantly reduced compared to the control group (1.34±0.43g, 3.26±0.39g, respectively, p<0.05). Vascular response to 40 mM KCl show that, slight decrease in the hypethyroidism group relative to control (1.89±0.19g, 2.17±0.14g vs, respectively).Conclusions: The decrease in the vascular response in this study suggests the decrease in vascular resistance in hyperthyroidism, and can be explained in part by changes in endothelial cell function

Investigating the individual and combined effects of coenzyme Q10 and vitamin C on CLP-induced cardiac injury in rats

Abstract Sepsis-induced cardiac injury represents a major clinical challenge, amplifying the urgency for effective therapeutic interventions. This study aimed to delve into the individual and combined prophylactic effects of Vitamin C (Vit C) and Coenzyme Q10 (CoQ10) against inflammatory heart injury in a cecal ligation and puncture (CLP) induced polymicrobial sepsis rat model. Thirty adult female Sprague–Dawley rats were randomly divided into five groups: Control, CLP, Vitamin C, CoQ10, and Vit C + CoQ10, each consisting of six rats. Treatments were administered orally via gavage for 10 days prior to the operation. Eighteen hours post-sepsis induction, the animals were euthanized, and specimens were collected for analysis. The study examined variations in oxidative (TOS, OSI, MDA, MPO) and antioxidative markers (TAS, SOD, CAT, GSH), histopathological changes, inflammatory cytokine concentrations (TNF-α, IL-1β), nitric oxide (NO) dynamics, and cardiac indicators such as CK-MB. Impressively, the combined regimen markedly diminished oxidative stress, and antioxidative parameters reflected notable enhancements. Elevated NO levels, a central player in sepsis-driven inflammatory cascades, were effectively tempered by our intervention. Histological examinations corroborated the biochemical data, revealing diminished cardiac tissue damage in treated subjects. Furthermore, a marked suppression in pro-inflammatory cytokines was discerned, solidifying the therapeutic potential of our intervention. Interestingly, in certain evaluations, CoQ10 exhibited superior benefits over Vit C. Collectively, these findings underscore the potential therapeutic promise of Vit C and CoQ10 combination against septic cardiac injuries in rats

Determination of acetylcholinesterase enzyme activity in liver tissues of rats with hyperthyroidism

Determination of Acetylcholinesterase Enzyme Activity in Liver Tissues of Rats with Hyperthyroidis

Determination of glutathione reductase enzyme activity in melatonin administered rats's heart tissue

Determination of Glutathione Reductase Enzyme Activity in Melatonin Administered Rats's Heart Tissu

Enhanced Efficacy of Resveratrol Loaded Silver Nanoparticle in Attenuating Sepsis-Induced Acute Liver Injury: Modulation of Inflammation, Oxidative Stress, and SIRT1 Activation.

Sepsis-induced acute liver injury is a life-threatening condition involving inflammation, oxidative stress, and endothelial dysfunction. In the present study, the preventive effects of resveratrol (RV) alone and RV-loaded silver nanoparticles (AgNPs + RV) against sepsis-induced damage were investigated and compared in a rat model of polymicrobial sepsis induced by cecal ligation and puncture (CLP). Rats were divided into four groups: Sham, CLP, RV, and AgNPs + RV. Pro-inflammatory cytokines (TNF-alpha, IL-1 beta, IL-6), nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kappa B) activation, presepsin, procalcitonin (PCT), 8-hydroxy-2 '-deoxyguanosine (8-OHDG), vascular endothelial growth factor (VEGF), and sirtuin-1 (SIRT1) levels were assessed to determine the treatments' effects. AgNPs + RV treatment significantly reduced pro-inflammatory cytokines, NF-kappa B activation, presepsin, PCT, 8-OHDG, and VEGF levels compared with the CLP group, indicating attenuation of sepsis-induced liver injury. Both RV and AgNPs + RV treatments increased SIRT1 levels, suggesting a potential role of SIRT1 activation in mediating the protective effects. In conclusion, AgNPs + RV treatment demonstrated extremely enhanced efficacy in alleviating sepsis-induced liver injury by modulating inflammation, oxidative stress, and endothelial dysfunction, potentially mediated through SIRT1 activation. In this study, the effect of AgNPs + RV on sepsis was evaluated for the first time, and these findings highlight AgNPs + RV as a promising therapeutic strategy for managing sepsis-induced liver injury, warranting further investigation