Engineering design of in vitro mono-culture model of blood-brain barrier and investigation of methotrexate permeability on the model [Kan-Beyin bariyerinin in vitro mono-kültür modelinin mühendislik tasari{dotless}mi{dotless}ni{dotless}n yapi{dotless}lmasi{dotless} ve model üzerinde metotreksat geçirgenli?inin ¯ncelenmesi]

2014 18th National Biomedical Engineering Meeting, BIYOMUT 2014 -- 16 October 2014 through 17 October 2014 -- 110485Blood-brain barrier (BBB) is a control mechanism that limits the diffusion of many substances to central nervous system through blood, and governs the nutrient diffusivity. This barrier is among the main risk factors for treatment of neurodegenerative diseases, because most drugs designed are protein based, and are either blocked by BBB, or lose their bioavailability significantly. In this study, an in vitro BBB model was designed for testing therapeutics for neurodegenerative diseases, and methotrexate drug permeability was investigated. In the BBB model design step, poly-caprolactone fiber surfaces were prepared by electrospinning to be used as support membrane for cells. The fiber morphology and sizes were determined using polarizing microscopy. Human umbilical vein endothelial cells (HUVEC) and C6 glioma cells were cultured on either side of this membrane. Model's proximity to in vivo models was tested by home-designed transendothelial electrical resistance measuring device; and nicotine was used as a positive control and albumin as a negative control. The effect of Methotrexate was determined indirectly by vitality test, MTT, for MCF-7 breast cancer cells seeded on the bottom of the well plates. The model's proximity to models in the literature and natural blood-brain barrier was specified relatively. © 2014 IEEE

Oral health status and its relation with medication and dental fear in children with attention-deficit hyperactivity disorder

Objective: The objective of this study was to determine the ora-dental health and its relation with medication and dental fear in a group of Turkish children with attention-deficit hyperactivity disorder (ADHD).Subjects and Methods: The levels of dental fear of children were determined with The Dental Subscale of Children’s Fear Survey Schedule (CFSS-DS). The oral and dental health evaluation was performed. This study included a total of 117 children aged between 6 and 15 years and they were examined under two groups as “ADHD” (n = 59) and “Control” (n = 58). Ora-dental health variables were compared between the groups and were also analyzed in accordance with dental fear and medication.Results: ADHD children and the control group exhibited similar CFSS-DS scores (15–32). No significant differences existed in df(t)/df(s), DMF(T)/DMF(S), d/D values, and presence of the white spot lesions. ADHD children’s Mutans streptococci and Lactobacillus quantities were found significantly higher than the control group. The incidence of parafunctional habits of the ADHD children was also found high.Conclusions: ADHD children that were medicated exhibited similar dental caries prevalence and periodontal health status. Although ADHD group had similar dental-periodontal health status and dental fear level with the control group and using ADHD medicines did not make a significant effect on the ora-dental health parameters, the patients should be carefully followed up because they were categorized in high caries risk groups.Keywords: Attention‑deficit hyperactivity disorder, clinical study, dental fear, medication, ora‑dental healt