Identification and quantification of some wine phenolic acids by high-performance liquid chromatography equipped with electrochemical detector

Phenolic acids (gallic acid, p-hydroxybenzoic acid, syringic acid, 2,3-dihydroxybenzoic acid, ferulic acid, p-coumaric acid and vanillic acid) in red (Cabernet Sauvignon, Cabernet Franc, Merlot, Syrah, Carignan, Grenache, Bogazkere) and white wine (Ugni Blanch, Chardonnay, Narince) were analyzed electrochemically (+0.65 V vs. Ag/AgCl) by HPLC (Aglient 1100 series) on C 18 silica column (Hichrom 5 C18, 7.75 × 300 mm, 5 µm particle size). The eluent used was methanol / 0.01 N phosphoric acid (30/70 v/v). The use of electrochemical detector (ECD) for wine phenols under isocratic conditions has not been reported yet. The evaluation of phenolic acids content in white and red wines brought about the importance of variety characteristics of grapes used in wine production. Changes in phenolic acids contents during two years production of Cabernet Frank, Cabernet Sauvignon and Carignan wines were determined to be caused by aging mechanisms

Trace determination of DNA in an amaryllidaceae plant, Narcissus tazetta by square-wave stripping voltammetry based on guanine signal

This study is, to our knowledge, the first application of electrochemical DNA biosensor for the quantification of DNA in plant extracts. The DNA biosensor was assembled by immobilizing the double-stranded fish sperm DNA on the surface of a singleuse pencil graphite electrode. Square-wave voltammetric method with the baseline correction was carried out to evaluate the oxidation signal of the guanine base. The experimental variables such as solution pH, buffer concentration, immobilization time, stirring speed, and square-wave parameters were optimized. The extremely low detection limit (0.36 ng/mL) was coupled to a good surface-to-surface reproducibility (a relative standard deviation of 8.4% for 7 repetitive measurements of 40 ng/mL). The renewable and low-cost DNA biosensor developed in this study using pencil graphite electrode was applied to the determination of DNA isolated from an Amaryllidaceae plant, Narcissus tazetta

Newborn hearing screening results of refugees living in our city and the factors affecting the results

PubMed ID: 31129457Objectives: To investigate the changes in the risk factors affecting the results of the Newborn Hearing Screening (NHS) and the hearing test results of the Syrian refugees in our city. Methods: Syrian and Turkish newborns, born in our hospital between 01.01.2016 and 31.12.2017 and referred to our hospital from environmental hospitals for NHS, were included in this study. NHS results and risk factors were analyzed. Results: 786 Syrian and 7230 Turkish newborns were included in this study. 53 (6,74%) infants referred in both ears, 26 (3,30%) infants in the one ear. There was a significant relationship between the presence of hearing loss and the history of intensive care unit admittance, presence and absence of low birth weight and neonatal icterus at Syrian newborns. In the same period, 20 (0,3%) Turkish infants referred bilaterally and 45 (0,6%) newborns unilaterally (25 right ear, 20 left ear). There was a significant difference between Turkish and Syrian newborns in terms of very low and low birth weight and intensive care unit admittance. Conclusions: The rate of hearing loss in Syrian refugee patients is quite high. Pregnant refugee women who are forced to migrate because of war face many risk factors and these people need to be included into the newborn hearing screening programs in the country where they took refuge in. © 2019 Elsevier B.V