Sharp Curvature of Premolar Resulting in External Apical Root Resorption of the Neighbouring Molar

This case report describes an external apical root resorption resulted from the unusual root morphology of the neighbouring tooth. A 28-year-old female was referred to the department of endodontics with a complaint of intense pain in her maxillary second premolar tooth. The clinical and radiographical evaluation revealed an external apical resorption in the mesial root of the maxillary first molar, which shows close proximity to the severely curved root of the premolar. A successful root canal treatment of the premolar was performed using anticurvature filing method. However, molar tooth received no curative treatment. One-year followup of the apical external resorption did not show any progression. External apical root resorption affecting single permanent tooth may be induced from the pressure exerted during the eruption of the adjacent tooth with unusual root morphology. The preferred approach for the management of such apical resorption cases includes long-term observation and no curative treatment

IDENTIFICATION OF TREPONEMA DENTICOLA, PORPHYROMONAS GINGIVALIS AND THEIR VIRULENCE FACTORS IN INFECTED ROOT CANALS BY MOLECULAR MICROBIOLOGIC TECHNIQUES

Bu çalışmanın amacı, enfekte kök kanallarında Treponema denticola, Porphyromonas gingivalis ve bu patojenlerin msp ve prtC genlerinin varlığı ve prevalanslarının Real-time PCR ile belirlenmesidir. Bu iki endodontopatojen ve virulans genleri ile klinik semptomlar arasındaki ilişki de çalışmamızda değerlendirilmiştir. 60 hastadan elde edilen enfekte pulpalar, akut apikal periodontitis, kronik apikal periodontitis ve akut apikal abse olmak üzere üç gruba ayrıldı. DNA ekstraksiyonunun ardından örnekler, T.denticola ve P.gingivalis için özgül primer ve problar kullanılarak Real-time PCR ile incelendi. P.gingivalis DNA sının pozitif saptandığı örneklerde, kollajenaz geninin belirlenmesi amacıyla prtC ye özgül bakteriyel primerler ve TaqMan problar kullanılarak Real-time PCR gerçekleştirildi. T.denticola nın pozitif saptandığı örneklerde, msp geninin belirlenmesi amacıyla konvansiyonel PCR uygulandı. T.denticola, incelenen tüm örneklerin % 44.1 inde, akut apikal periodontitis grubundaki 26 vakanın 12 inde, akut apikal abse grubundaki 7 vakanın birinde, kronik apikal periodontitis grubundaki 27 vakanın 12 inde saptandı. P.gingivalis, incelenen tüm örneklerin %16.7 inde, akut apikal periodontitis grubundaki 26 vakanın 3 ünde, akut apikal abse grubundaki 7 vakanın ikisinde, kronik apikal periodontitis grubundaki 27 vakanın 5 inde saptandı. msp DNA sı T.denticola nın pozitif saptandığı 26 adet örneğin 7 inde, prtC DNA sı P.gingivalis in pozitif saptandığı 10 örneğin 9 unda saptandı. Bakteriler ve virülans genleri ile üç klinik patolojik durum arasındaki ilişki istatistiksel olarak anlamlı bulunmadı. (p>0.05) izmir gundem komik videolar cizgi film izle cizgi film 3d oyunlar oyunlar The aim of this study was to assess the presence and prevalence of T. denticola, P. gingivalis and their msp and prtC genes in the infected root canals by Real-time PCR. The relationship between the presence of these two endodontopathogens, their virulence genes and the clinical symptomatology was also evaluated. The infected pulps of 60 patients were divided into three clinical groups: acute apical periodontitis, chronic apical periodontitis, acute apical abscess. DNA was extracted from samples which were further analysed using the primers and probes that are specific for T. denticola and P.gingivalis. In the samples that yielded positive P.gingivalis DNA amplicon, in order to detect the collagenase gene Real-time PCR were performed using prtC specific bacterial primers and Taq Man probes. Conventional PCR method was employed to identify msp in the samples that were positive for T.denticola. T.denticola was found in 44.1% of the total samples, in 12 of 26 cases with acute apical periodontitis, one of seven cases with acute apical abscess, and 12 of 27 cases with chronic apical periodontitis. P.gingivalis was observed in 16.7% of the total, three of 26 cases with acute apical periodontitis, two of seven cases with acute apical abscess, and five of 27 cases with chronic apical periodontitis. msp DNA was detected in seven of 26 T.denticola positive samples, and prtC DNA was detected in nine of ten P.gingivalis positive samples. The association between the presence of the bacteria, the virulence genes and three clinical pathologies was not statistically significant (p>0.05)

Effect of various calcium hydroxide removal protocols on the dislodgement resistance of biodentine in an experimental apexification model

Background/purpose: Residual calcium hydroxide (CH) in the root canal dentine walls may influence the adhesion of tricalcium silicate-based materials. The aim of this study is to evaluate the effect of various CH removal protocols on the dislodgement resistance of biodentine from simulated immature root canals in an experimental apexification model Materials and methods: CH was applied to 120 simulated immature root canals. The samples were divided into 12 experimental groups (n Z 10) according to the applied irrigation protocols used for the removal of CH: Group 1: Sodium hypochlorite (NaOCl), Conventional needle irrigation (CNI); Group 2: NaOCl, EndoActivator; Group 3: NaOCl, XP-endo Finisher; Group 4: NaOClEthylenediaminetetraacetic acid (EDTA), CNI; Group 5: NaOCl-EDTA, EndoActivator; Group 6: NaOCl-EDTA, XP-Endo Finisher; Group 7: NaOCl+etidronic acid (HEBP), CNI; Group 8: NaOCl+HEBP, EndoActivator; Group 9: NaOCl+HEBP, XP-endo Finisher; Group 10: NaOClPeracetic acid (PAA), CNI; Group 11: NaOCl-PAA, EndoActivator; Group 12: NaOCl-PAA, XP-endo Finisher; Control Group: CH was not applied. Biodentine was placed at the apical thirds of 130 immature root canals. Vertical loading was applied to biodentine fillings inside the dentin discs. Maximum force to dislodge the material was statistically analyzed with ANOVA. Results: The control, NaOCl+HEBP (CNI, EndoActivator, XP-endo Finisher) and NaOCl-PAA (EndoActivator, XP-endo Finisher) groups exhibited the lowest dislodgement resistance values (p < 0.001). When used CNI, irrigation with NaOCl+HEBP resulted in lower resistance to dislodgement of biodentine compared to NaOCl, and NaOCl-EDTA (p < 0.001). Conclusion: Adhesion of apical barrier materials to root canal dentine can be influenced by the irrigation protocols used for CH removal. & ordf; 2020 Association for Dental Sciences of the Republic of China. Publishing services by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons. org/licenses/by-nc-nd/4.0/)

The effects of ethylenediamine tetraacetic acid, peracetic acid, and etidronic acid on the tissue dissolution capacity of sodium hypochlorite: in vitro

Objective: The aim of this study was to evaluate the effects of 18% ethylenediamine tetraacetic acid (EDTA), 2% peracetic acid (PAA), and 9% etidronic acid (HEBP) on the organic tissue dissolution activity of sodium hypochlorite (NaOCl). Materials and Method: Sixty samples with similar weight and dimensions were obtained from bovine muscle tissue. The tissue samples were blotted dry on filter paper and weighed with a precision balance. The specimens were immersed in following solutions: (1) 2 mL 2.5% NaOCl, (2) 1 mL 5% NaOCl + 1 mL 18% EDTA, (3) 1 mL 5% NaOCl + 1 mL 2% PAA, (4) 1 mL 5% NaOCl + 1 mL 9% HEBP. The specimens were then dried and weighed again. The weight loss of each specimen incubated in the test solutions was measured at 30 and 60 min. The data were statistically analyzed with one-way ANOVA and post-hoc Tukey tests. Results: Use of NaOCl (5%) together with 18% EDTA resulted in minimal tissue dissolution capacity compared to the other groups at both time points (p<0.001). The tissue dissolution capacity of NaOCl was also affected by 9% HEBP. The greatest tissue weight reduction values were obtained in the NaOCl+PAA group at 30 minutes (p<0.001). At 60 min, NaOCl and NaOCl+PAA groups exhibited the greatest tissue dissolution capacity (p<0.001); no significant difference was found between these two groups (p=0.169). Conclusion: EDTA and HEBP decreased the tissue dissolution capacity of NaOCl, whereas PAA did not have any negative effect on the ability of NaOCl to dissolve the organic tissue

Effect of ethylenediamine tetraacetic acid and etidronic acid on the surface roughness of Biodentine: in vitro

Objective: The aim of this study was to evaluate the effect of 9% etidronic acid (HEBP) and 17% ethylenediamine tetraacetic acid (EDTA) on the surface roughness of Biodentine. Materials and Method: Biodentine (Septodont) was mixed according to the manufacturer’s instructions. Briefly, five drops of liquid were added into the capsule containing the powder. Then the capsule was placed in a triturator for 30 sec. The prepared mixture was placed into a mold (diameter: 5 mm, depth: 3 mm). The Biodentine surfaces were polished with silicon carbide abrasive papers. The surface roughness of 30 samples was measured at baseline using a portable surface roughness tester. For this purpose, a 5 mN force was applied onto three different locations of the samples with a speed of 0.8 mm/sec. The samples were divided into two groups according to the irrigation solution (n=15); first group was treated with 9% HEBP, and the second group was treated with 17% EDTA. The surface roughness of the samples was measured again after 1 and 2 min of irrigant application. Data were statistically analyzed using one-way ANOVA and independent sample t-test. Results: For HEBP, no significant difference was found between the surface roughness values at 0., 1., and 2 min (p=0.107; ANOVA). For EDTA, the surface roughness value at 1 min was significantly greater than the baseline value (p<0.001; t-test). The surface roughness changes at the two time periods were significantly different between the EDTA and HEBP groups (p=0.003 for 0-1 min passage, p=0.021 for 1-2 min passage). Conclusion: The use of 17% EDTA may result in deterioration of Biodentine’s surface during perforation repair and root canal treatment

Primer kok kanal enfeksiyonlarında Tannerella forsythia prevalansının 16S rRNA PCR ile incelenmesi

Bu çalışmanın amacı Tannerella forsythia’nın primer kök kanal enfeksiyonlarındaki prevalansını ve bu bakterinin değişik formlardaki periradiküler hastalıklar ile ilişkisini Polimeraz Zincir Reaksiyonu PCR yöntemi ile incelemektir. Örnekler, akut ve kronik formlardaki periradiküler hastalıklar ve abselerden elde edildi. Filtreli kolon DNA izolasyon kiti kullanılarak DNA ekstraksiyonu yapıldı ve 16S rRNA gen bazlı PCR yürütüldü. Verilerin istatistiksel analizinde Ki - kare testi kullanıldı. Akut apikal periodontitisli 26 vakanın 13’ünde %50 , akut periradiküler abseli 7 vakanın 3’ünde %42,8 ve kronik asemptomatik periradiküler lezyonlu 25 vakanın 2’sinde %8 olmak üzere toplam 58 vakanın 18’inde %31,3 Tannerella forsythia DNA’sı saptandı. Bakteri varlığı ile akut apikal periodontitis grubu arasındaki ilişki istatistiksel olarak önemli bulundu P=0.002 . Tannerella forsythia periradiküler hasarın akut semptomları ile ilişkili potensiyel bir endodontik patojen olarak düşünülmelidir

POSTERİOR KOMPOZİT VE AMALGAMLA RESTORE EDİLMİŞ ENDODONTİK TEDAVİLİ MAKSİLLER PREMOLAR DİŞLERİN KIRILMAYA DAYANIKLILIĞI

Endodontik tedavi görmüş dişlerin restorasyonu, restoratif diş hekimliğinin en çok tartışılan konular›ndan biri olmuştur. Bu çal›şman›n amac›, endodontik tedavi görmüş ve iki farkl› tipte materyalle restore edilmiş maksiller premolar dişlerin k›r›lma dayan›kl›l›ğ›n›n karş›laşt›r›lmas›d›r. Bu çal›şmada 30 adet çürüksüz insan dişi kullan›lm›şt›r. Dişler her biri 10 dişten oluşan rastgele 3 gruba ayr›lm›şt›r. İlk iki gruptaki dişlerin kanallar› #35’ya kadar genişletilip kon-gütta ve AH 26 ile lateral kondensasyon tekniği kullan›larak doldurulmuştur. Ard›ndan gütta perka minesement birleşiminin 2 mm alt›nda olacak şekilde bir rond frezle kald›r›lm›şt›r. Grup 1, yüksek bak›rl› amalgam; Grup 2, posterior kompozitle restore edilmiş; Grup 3’e kontrol grubu ise herhangi bir dental işlem uygulanmam›şt›r. Mekanik testler için universal test ayg›t› kullan›lm›şt›r. Tüm dişlere k›r›lma oluşana kadar kuvvet uygulanm›şt›r. Grup 1 için ortalama kuvvet 1125.40 N, Grup 2 için 983.62 N ve Grup 3 için 2110.58 N dur. Grup 1 ve grup 2 aras›nda istatistiksel olarak anlaml› bir fark bulunamazken p>0.05 , bu gruplar›n k›r›lma dayan›kl›l›ğ› Grup 3’ten anlaml› şekilde düşük bulunmuştur

Farklı kök kanal patlarının apikal mikrosızıntılarının değerlendirmesi

Bu çalışmanın amacı farklı kök kanal patlarının apikal mikrosızıntılarını boya sızıntısı yöntemiyle karşılaştırmaktır. 47 adet tek köklü diş, kök boyları 14 mm olacak şekilde mine-sement sınırında kronlarından ayrıldı. Tüm kökler ProTaper döner eğe sistemiyle apikal boyut F3 #30 olacak şekilde enstrümante edildi. 39 adet kök kanalı soğuk lateral kompaksiyon tekniği ile dolduruldu: Grup 1 n=13 : AH26+kon gütaperka. Grup 2 n=13 : EndoREZ+kon güta-perka. Grup 3 n=13 : iRoot SP+kon güta perka. Kalan 8 kök pozitif ve negatif kontrol olarak kullanıldı. Kökler 72 saat boyunca metilen mavisi solüsyonunda bekletildi ve longitudinal olarak ikiye ayrıldı. Apikal bölgedeki boya penetrasyonu steromikroskopla ölçüldü. Veriler, tek yönlü ANOVA ve post-hoc Tukey testi kullanılarak istatistiksel olarak incelendi. EndoREZ grubu istatistiksel olarak anlamlı şekilde iRoot SP’den daha fazla sızıntı gösterdi p=0.001