Kudret Narı (Momordica charantia Descourt.) Meyvesinden Saflaştırılan Peroksidaz Enzimi Kullanılarak Hibrit Nano Çiçekler Sentezlenmesi ve Direct Blue 1 Gideriminde Kullanılabilirlikleri

.Peroxidase enzymes are purified from different plant sources are used efficiently for the removal of dyes in industrial wastes. The fruit of bitter gourd (Momordica charantia), an inexpensive and easily accessible vegetable, is an important source of peroxidase. In this study, total protein content was found to be 0.485 mg/mL and peroxidase activity was found to be 2360.9 EU/mg as a result of 50% protein precipitation made from green bitter gourd. However; total protein amount was 0.232 mg/mL and free peroxidase activity was determined as 7719.30 EU/mg as a result of 60% protein precipitation made from ripe bitter gourd. Peroxidase enzymes which were purified from bitter gourd in different growth stages under optimum conditions showed higher enzymatic activity compared to free forms when immobilized via enzyme-inorganic hybrid nanoflower synthesis method. The highest peroxidase activity was seen in mature fruit and hybrid nanoflower form (19661, 6 EU/mg). In addition, the usability of hybrid nanoflowers was investigated compared to the free purified peroxidase for removal of Direct Blue 1 dye widely used in textile industry. It was determined that hybrid nanoflower form synthesized especially by using ripe bitter gourd peroxidase had more dye removal.

A new approach for green synthesis and characterization of Artemisia L. (Asteraceae) genotype extracts-Cu2 nanocomplexes (nanoflower) and their effective antimicrobial activity

.In this study, we have demonstrated the fabrication of novel organic-inorganic nanobio-antimicrobial agents called “nanoflowers” (NFs) and elucidate the increase in the antimicrobial activity of NFs. This is the first report that the NFs were formed of plant extracts as the organic components and copper (II) ions (Cu2+) as the inorganic component. The Artemisia L. (Asteraceae) methanol extracts from three genotypes including A. absinthium L. (Aa), A. vulgaris L. (Av) and A. ludoviciana Nutt. (Al) were selected in the NF synthesis. The effect of the plant extract concentrations on the morphology of NFs was examined. Most regular and uniform flower-shaped morpholo- gies were observed when a concentration of 0.1 mg mL-1 plant extract was used in the synthesis of NFs. The syntesized NFs were characterized with several techniques such as scanning electron microscopy (SEM), Fourier transform infrared spectrometer (FT-IR), energy-dispersive X-ray (EDX) and X-ray diffraction analysis (XRD). The NFs exhibited much antimicrobial activity against the pathogens even at low concentrations compared to the extracts. The MICs and MBCs values for NFs were found to be range between 0.4 to 40 μg mL-1 and 40 to 400 μg mL-1 while those values for Aa, Av and Al extracts were ranged from 500-2000 μg mL-1 and 1000-4000 μg mL-1 for the studied pathogens, respectively.

Volume XCI, Number 25, May 12, 1972

In this study, molecularly imprinted polymer membranes were synthesized for the recognition and adsorption of quercetin. For this, quercetin imprinted polymeric membranes [p(HEMA-MAH)] (Poly(2-hydroxyethyl methacrylate-co-N-methacryloly-l-histidinemethylester) were synthesized by UV polymerization technique using HEMA and MAH as monomers. Synthesized polymeric membranes were characterized with SEM, FTIR and swelling test. Characterized membranes were used for the direct adsorption of quercetin in a batch system. Quercetin adsorption conditions were optimized by using the quercetin imprinted polymeric membrane by altering the pH, temperature and initial quercetin concentration of the adsorption medium. Effect of adsorption time was also studied for up to 180 min. The optimum pH and temperature was determined between 4.0 and 45 degrees C. Maximum adsorbed amount of quercetin onto quercetin imprinted poly(HEMA-MAH) membrane was found to be as 299.6 mg/g membrane using the initial quercetin concentration of 2.0 mg/ml. Adsorbed quercetin was desorbed from the polymeric membranes with isopropyl alcohol with desorption yield of 98.3%. and repeated usability of the quercetin imprinted polymeric membranes was fallowed for 7 adsorption/desorption cycles. At the end of the 7(th) reuse, quercetin adsorption capacity of the quercetin imprinted poly(HEMA-MAH) membranes decreased only about 10%

PD-L1 expression in immune cells is a favorable prognostic factor for nasopharyngeal carcinoma

Background: Programmed death-ligand 1 (PD-L1) has been determined as a reliable prognostic factor for various malignancies. In this study, we aimed to determine the prognostic effect of PD-L1 expression in tumor-infiltrating immune cells (TIICs) of nasopharyngeal carcinoma (NPC) patients.Methods: Seventy patients diagnosed with non-metastatic NPC were included in the study. PD-L1 expression on immune cells was analyzed by immunohistochemical method. Patients were categorized into two groups according to the PD-L1 expression level in TIICs (level of PD-L1 staining ≥5% positive vs <5% negative).Results: Median follow-up period was 34 months (range = 1 - 188). 1 and 2 years survival rate were found as 75% and 63% in PD-L1 negative TIICs group (47%), and 85% and 83% in PD-L1 positive TIICs group (53%), respectively. PD-L1 positivity in immune cells (ICs) was detected in 53% of the patients. The survival rate was found better in the PD- L1 positive group compared to the negative group (P = 0.049).Discussion: In conclusion, the survival rate was found significantly better in the PD-L1 positive TIICs group, compared to the negative group