Mualla Eyuboğlu

Taha Toros Arşivi, Dosya No: 185, 186) Eyuboğlu, Orhan-Cemal-Osman Zeki-Bedri Rahmi-Mualla-SabahattinUnutma İstanbul projesi İstanbul Kalkınma Ajansı'nın 2016 yılı "Yenilikçi ve Yaratıcı İstanbul Mali Destek Programı" kapsamında desteklenmiştir. Proje No: TR10/16/YNY/010

The effect of folate on ischemia/reperfusion injury in a rat adnexal torsion model

Purpose The ischemia/reperfusion (I/R) injury of ovaries in adnexal torsion may have inadvertent consequences. Many agents have been studied in terms of their ability to prevent reperfusion damage to ovaries in suspected cases. In this study, folic acid, known to have antioxidative properties, was investigated to determine whether it played a role in the prevention of I/R damage in a rat ovarian torsion model.Methods In this experimental study, 40 female adult Wistar-Albino rats were randomly divided into fve groups as control, ischemia, I/R, Fol2 (2 mg/kg folic acid), and Fol4 (4 mg/kg folic acid). In the Fol2 and Fol4 groups, folic acid was intra peritonelly administered 30 min before reperfusion. Blood samples were obtained from the tails of each rat at the second hour of reperfusion.Results The total oxidant status (TOS), total antioxidant status, cystatin C and folic acid levels of the fve groups were investigated. Folic acid in 2 mg/kg dose could moderately increase the serum folic acid concentration (15.75–19.95 ng/ml, p0.05), although there was no statistical diference in TOS levels (p=0.07). Folic acid in 4 mg/kg dose, could signifcantly increase the serum folic acid concentration (15.75–37.65 ng/ml). However, it did not signifcantly reduce the level of cystatin C (0.18–0.19 µg/L, p>0.05), and did not improve oxidative stress injury (76.05–130.58, p>0.05).Conclusion Folic acid in 2 mg/kg dose might improve the ovarian I/R injury though this was not statistically signifcant. Further studies are required to reach a defnitive conclusion about the protective efect of folic acid in I/R injury

Olağandışı HbA1c düzeyi gözlenen tip 1 diyabetli bir olgunun değerlendirilmesi

The HbA1c test provides information about blood glucose levels of previous months depending on the erythrocyte lifetime when monitoring diabetic patients. However, various factors such as HbF and other hemoglobin variants can interfere with the measurement of HbA1c. In this study, an unexpectedly high HbA1c level was observed in a patient with type 1 diabetes. In the hemoglobin chain analysis, which explained the reason for the high value, high fetal hemoglobin levels were detected and interfered with HbA1c measurement with the HPLC method. This finding was in concordance with the literature. As a conclusion, it should be considered that hemoglobinopathy might be found in the patients who have higher levels of HbA1c which is not in agreement with the blood glucose. Thus, it can be said that HbA1c test is not a good marker for monitoring such diabetic patients. In such cases, fructosamine or other glycated end products may be a more reliable marker.HbA1c testi, diyabetik hastalarda eritrosit yaşam süresine bağlı olarak son birkaç aylık kan şekeri düzeyi hakkında bilgi veren bir testtir. Çeşitli yöntemlerle ölçülen HbF ya da diğer hemoglobin tipleri HbA1c ölçüm sonuçlarını etkileyebilir. Bu çalışmada tip 1 diyabetli bir hastada beklenmedik yüksek HbA1c düzeyi gözlendi. Nedenini açıklamak için gerçekleştirilen Hb zinciri analizinde, yüksek HbF düzeyinin HPLC metodu ile yapılan HbA1c ölçümünü etkilediği tespit edildi. Bu bulgu literatür ile de uyum göstermekteydi. Sonuç olarak, kan şekeri ile uyumu düşünüldüğünde yüksek bulunan HbA1c düzeyleri için hastalarda hemoglobinopati bulunabileceği göz önüne alınmalıdır. Bu durumda HbA1c testinin böyle diyabetik hastaların takibi için iyi bir rehber olmadığı söylenebilir. Bu gibi vakalarda, fruktozamin veya diğer glukozile son ürünler daha güvenilir izlem belirteci olabilir

Galectin-3, IL-1A, IL-6, and EGF levels in corneal epithelium of patients with recurrent corneal erosion syndrome

Purpose: To determine the galectin-3 (Gal3), interleukin-1 (IL-1), interleukin-6 (IL-6), and epidermal growth factor (EGF) levels in corneal epithelium of patients with recurrent corneal erosion (RCE) syndrome and compare them with healthy controls. Methods: In this prospective interventional case control study, 32 eyes of 32 patients with RCE syndrome who had corneal epithelial erosions and 28 eyes of 28 healthy participants scheduled for photorefractive keratectomy (control group) were included. Exclusion criteria included corneal dystrophies, ectasia, dry eye, previous ocular surgery or topical medications, and systemic diseases. Epithelial samples were obtained during epithelial debridement in the study group and mechanical epithelial keratectomy in the control group. Galectin-3 levels were studied by the chemiluminescent microparticle immunoassay method. IL-1, IL-6, and EGF levels were determined using corresponding ELISA kits. Results: The median Gal3 levels were 132.25 ng/mL in the study group and 106.50 ng/mL in the control group. The median IL-1 and IL-6 levels were 6.24 pg/mL and 10.16 pg/mL, respectively, in the study group which were higher than that in the control group. The median EGF level in the study group was lower than that the control group with 1.30 pg/mL versus 2.67 pg/mL. In the control group, there was a significant positive correlation between EGF and IL-6 (r = 0.554; P = 0.040). A similar correlation was not observed in patients with RCE (r = -0.071; P = 0.794). Conclusions: The lack of increased EGF expression and the imbalance between growth factors, adhesion molecules, and interleukins may be the reason for the impaired wound healing response in RCE syndrome

Role of serum 25-hydroxyvitamin D levels and vitamin D receptor gene polymorphisms in patients with rosacea: a case-control study

Background Vitamin D has significant effects on the immune system and thereby on the pathogenesis of rosacea. However, there is a lack of information on the vitamin D status and vitamin D receptors (VDRs) of patients with rosacea. Aim To evaluate the role of vitamin D in rosacea susceptibility. Methods A case-control study was conducted, enrolling patients with rosacea and healthy controls (HCs). Five VDR gene single nucleotide polymorphisms (SNPs) (Cdx2, FokI, ApaI, BsmI and TaqI) and serum 25-hydroxyvitamin D-3 [25(OH)D-3] levels were compared between patients and HCs. Results The study enrolled 60 patients (M/F: 14/46) and 60 age- and sex-matched HCs (M/F: 14/46). Age (mean +/- SD) was 48 +/- 11 years for both groups. The serum 25(OH)D-3 levels (median +/- interquartile range) were higher in patients with rosacea (12.9 +/- 6.8 ng/mL) than in HCs (10.5 +/- 3.7 ng/mL) (P < 0.001). Subjects with high serum 25(OH)D-3 levels had a 1.36-fold increased risk of rosacea (95% CI 1.17-1.58). Heterozygous and mutant ApaI polymorphisms increased rosacea risk by 5.26-fold (95% CI 1.51-18.35) and 3.69-fold (95% CI 1.19-11.48), respectively, whereas mutant TaqI polymorphisms decreased the risk by 4.69 times (95% CI 1.37-16.67). Heterozygosity for Cdx2 alleles increased rosacea risk, whereas wildtype ApaI and mutant TaqI alleles decreased it. Conclusions The present study suggests that an increase in vitamin D levels may contribute to the development of rosacea. ApaI and TaqI polymorphisms, and heterozygous Cdx2, wildtype ApaI and mutant TaqI alleles were significantly associated with rosacea. These results indicate a possible role of vitamin D and VDR pathways in the pathogenesis of rosacea, although causality could not be assessed.Turkish Society of Dermatology, Ankara [2015/112]This study was supported by The Turkish Society of Dermatology, Ankara (Grant no: 2015/112)

The effect of serum electrolyte disturbances and uric acid level on the mortality of patients with acute exacerbation of chronic obstructive pulmonary disease

Objectives: The aim of the study was to determine the prevalence of electrolyte and uric acid disturbances and their effects on mortality in patients with acute exacerbations of chronic obstructive pulmonary disease (AECOPD). Materials and methods: This study included all consecutive AECOPD patients who were managed at our Chest Diseases department between May 2017 and December 2017. Medical records of all the subjects were reviewed, and data were collected retrospectively. Eighty-one patients with AECOPD and 103 subjects in the control group were enrolled retrospectively. The association between the COPD and control groups and biochemical parameters in patients with and without long-term oxygen therapy and noninvasive mechanical ventilation treatment in COPD patients were compared with mortality. Results: Serum magnesium, phosphorus, potassium, sodium, and calcium (Ca levels were higher in control subjects than in COPD patients (p=0.006, p=0.015, and p<0.001, respectively). While serum levels of Ca and K were significantly lower and serum level of uric acid was higher in deceased COPD patients than in alive AECOPD patients (p=0.023, p=0.001, and p=0.033, respectively), serum levels of Mg, P, and other biochemical parameters were similar. Conclusion: Serum Ca, K, and uric acid levels during the exacerbation period were significant predictors of mortality in COPD patients. In conclusion, the levels of these parameters should be measured and corrected during AECOPD treatment to decrease mortality

New inflammatory parameters in laryngopharyngeal reflux

Objectives: To investigate new inflammatory markers in patients with laryngopharyngeal reflux and determine whether these inflammatory parameters change in response to laryngopharyngeal reflux treatment. Methods: Complete blood count was evaluated to obtain platelet count and mean platelet volume and calculate neutrophil-to-lymphocyte ratio and platelet-to-lymphocyte ratio. Laryngopharyngeal reflux patients underwent three-month lansoprazole treatment. Results: The study included 45 laryngopharyngeal reflux patients (9 men (20 per cent); mean age, 37.4 ± 11.6 years) and 35 healthy age- and sex-matched controls (7 men (20 per cent); mean age, 38.6 ± 8.9 years). The study group had significantly higher platelet-to-lymphocyte ratios and lower mean platelet volumes than the control group (p = 0.004 and p = 0.047, respectively). There was a significant correlation between platelet-to-lymphocyte ratios and initial inflammatory symptoms (reflux symptom index, p = 0.025; reflux finding score, p = 0.013). There was also a significant correlation between mean platelet volume increase and symptom resolution in the first and third months of treatment (p = 0.04 and p = 0.03, respectively). Conclusion: Platelet-to-lymphocyte ratio, a new inflammatory marker of chronic inflammation, was significantly higher in laryngopharyngeal reflux patients. Moreover, these patients had significantly lower mean platelet volume values, which increased with post-treatment symptom improvement

Serum Ischaemia-modified albumin concentration in hyperemesis gravidarum

Introduction: The role of oxidative stress in the pathogenesis of hyperemesis gravidarum (HEG) has been demonstrated in a lot of studies. The present study aimed to compare the ischaemia-modified albumin (IMA) serum levels of patients diagnosed with HEG with healthy pregnant women, and to investigate whether oxidative stress caused increased serum IMA in HEG. Methods: Pregnant women were classified into a group diagnosed with HEG (n=45) and an age- and body mass index-matched control group without HEG (n=45). Serum IMA levels were assessed by the enzyme-linked immunosorbent assay (ELISA) method. Results: Serum IMA levels were higher in the HEG group than the control group (HEG: 8.2 +/- 0.2 ng/mL, control: 6.9 +/- 03 ng/mL, p<0.001). Conclusion: We found that HEG was related to increased maternal serum IMA levels. The high levels of IMA in HEG can he considered as a reflection of increased oxidative stress

The association between ischemia modified albumin and placental histopathology in uncomplicated term deliveries

Ischemia modified albumin (IMA) is a marker of ischemia elevated in different clinical conditions and its use for hypoxia in perinatology is of current interest. We aimed to investigate the association between maternal and cord blood IMA levels and placental histopathological findings in uncomplicated term deliveries. In this study, placental histopathological evaluation in uncomplicated deliveries that ended with healthy newborns revealed 80.6% vasculopathy. The results support the hypothesis that hypoxia exceeding the placental reserve ends with fetal compromise. Moreover, the presence of maternal vasculopathy in placenta is not correlated with maternal and fetal IMA levels

CRP at early follicular phase of menstrual cycle can cause misinterpretation for cardiovascular risk assessment

Objective: C-reactive protein (CRP) is a well-known marker of infl ammation and infection in clinical practice. This study is designed to evaluate CRP levels in diff erent phases of menstrual cycle, which might end up with misleading conclusions especially when used for cardiovascular risk assessment. Methods: Twenty-seven women were eligible for the cross-sectional study. Venous blood samples from each participant were collected twice during the menstrual cycle. The fi rst sampling was held at 2nd to 5th days of the menstrual cycle for FSH, estradiol, CRP, and sedimentation, and the second was done at 21st to 24th days of the menstrual cycle for measurement of progesterone, CRP, and sedimentation values. Results: CRP values were signifi cantly higher in the early follicular phase compared to luteal phase (1.8 mg/L [0.3–7.67] vs. 0.7 mg/L [0.1–8.3], p < 0.001, respectively). In both phases of the menstrual cycle, sedimentation rate was similar (12.1 ± 6.7 vs. 12.3 ± 7.7; p = 0.717, respectively). Conclusions: CRP levels in early follicular phase of the menstrual cycle (menstruation) are signifi cantly higher than CRP levels in luteal phase of the same cycle. In reproductive age women, detection of CRP for cardiovascular risk assessment during menstruation might not be appropriate