120 research outputs found
Investigation of the preventative roles of manganese superoxide dismutase Ala16Val gene polymorphism in coronary artery events
Amaç: Araştırmamızın amacı, mangan süperoksit dismutaz (MnSOD) genine ait Ala16Val polimorfizminin koroner arter hastalığı (KAH) olan kişilerdeki
genotip sıklıklarını belirlemek ve genotip-kalp damar hastalığı ve genotip-biyokimyasal parametreler ve serum MnSOD aktivitesi ile etkileşimin
değerlendirilmesidir.
Gereç ve yöntemler: MnSOD geni Ala16Val genotipleri kantitatif polimeraz zincir reaksiyonu yöntemi ile saptandı. Serum MnSOD aktivitesi ELİSA
yöntemi ile belirlendi.
Bulgular: MnSOD geni Ala16Val polimorfizmi için genotip sıklıkları KAH grubunda C/C (Homozigot yabanıl tip), C/T (Heterozigot), T/T (Homozigot
polimorfik tip) genotipler için sırasıyla %20.9, %41.9, %37.2 ve KAH olmayan grupta %15.5, %51.7, %32.8 olarak tespit edildi. Tüm çalışma grubundaki
mitokondriyal MnSOD aktiviteleri, MnSOD geni Ala16Val genotiplerine göre karşılaştırıldığında CC genotip taşıyıcılarının en yüksek, TT genotip
taşıyıcılarının en düşük MnSOD aktivitesine sahip olduğu belirlendi. Koroner arter hastalığı bulunmayan hastalarda MnSOD Ala16Val polimorfizminin
yağsız vücut kütlesi (p=0.01) ve yağ kütlesi (p=0.05) üzerine anlamlı etkileri olduğu saptandı. Ala16Val polimorfizminin heterozigot genotipe sahip KAH
hastalarında yağdan zengin gıdalarla beslenme alışkanlıkları diğer genotiplere kıyasla daha düşük iken, homozigot polimorfik genotipe sahip KAH
hastalarının diğer genotiplere kıyasla proteinden fakir beslenme alışkanlıklarını benimsedikleri görüldü.
Sonuç: Tüm çalışma grubu değerlendirildiğinde, MnSOD Ala16Val genotipleri ile beslenme alışkanlıkları arasında anlamlı bir ilişki bulunurken yağsız
vücut kütlesi üzerinde etkili bulunmamıştır.Objectives: The aim of this study was to determine the genotypic frequencies of manganese superoxide dismutase (MnSOD) gene Ala16Val
polymorphism frequencies and to evaluate the effects of MnSOD Ala16Val variation over coronary artery disease (CAD) in which people, biochemical
parameters and MnSOD activity.
Materials and methods: MnSOD gene Ala16Val polymorphisms were determined with quantitative polymerase chain reaction method. MnSOD
activity in serum were determined by ELISA method.
Results: The MnSOD gene Ala16Val genotype frequencies were determined respectively as 20.9%, 41.9%, 37.2% for homozygous wild genotype
(C/C), heterozygous genotypes (C/T), homozygous polymorphic genotype (T/T) in the CAD patients; whereas 15.5%, 51.7% and 32.8% for the patients
without CAD. In total study group, C/C genotype carriers were detected to have the highest, whereas T/T genotype carriers were detected to have the
lowest mitochondrial MnSOD activities when compared according to MnSOD gene Ala16Val genotypes. In the patients without CAD, MnSOD Ala16Val
polymorphism was found to have significant effects on lean body mass (p=0.01) and fat mass (p=0.05). In CAD patients with Ala16Val polymorphism
heterozygous genotype oil-rich eating habits were lower to that of other genotypes, whereas CAD patients with homozygous polymorphic genotype
was detected to prefer protein-poor diet.
Conclusion: When all the study group was evaluated, MnSOD Ala16Val genotypes were not found to have statistically significant relationship with
eating habits, but found to be effective only on lean body mass
Omentum adiposity is linked with resistin gene expression
Background: This study demonstrated site-specific adipose tissue resistin gene expression differences in
individuals with and without type 2 diabetes mellitus. The relationship between conventional drug therapy and adipose
tissue resistin gene expression was also determined. Paired omental and subcutaneous adipose tissues were excised
during elective surgery from morbidly obese and obese patients.
Methods: Resistin mRNA expressions were determined by qPCR. All tissue sections also were also analyzed for
their resistin and CD68 protein expressions by immunohistochemistry.
Results: No significant difference for omental and subcutaneous adipose tissue resistin mRNA expression levels
were found among morbidly obese and obese study groups. The omental adipocytes resistin mRNA expressions
increased with macrophage number both in the omental and subcutaneus fat. Resistin mRNA expressions of
the omental and subcutaneous fat were in positive correlation. As the omental adipocytes radius decreased, the
macrophage number increased in subcutaneous fat. In the omentum the adipocytes diameter and areas increased,
in correlation with macrophage number. The antidiabetic drug use was found to increase adipocyte size both in the
omentum and subcutaneous fat.
Conclusions: The higher resistin gene expression in the omental fat may induce the increase in size and number
of adipocytes, thus leading to elavation in omental fat mass
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