45 research outputs found

    Genome-Wide Analysis of Secondary Metabolite Gene Clusters in Ophiostoma ulmi and Ophiostoma novo-ulmi Reveals a Fujikurin-Like Gene Cluster with a Putative Role in Infection

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    The emergence of new microbial pathogens can result in destructive outbreaks, since their hosts have limited resistance and pathogens may be excessively aggressive. Described as the major ecological incident of the twentieth century, Dutch elm disease, caused by ascomycete fungi from the Ophiostoma genus, has caused a significant decline in elm tree populations (Ulmus sp.) in North America and Europe. Genome sequencing of the two main causative agents of Dutch elm disease (Ophiostoma ulmi and Ophiostoma novo-ulmi), along with closely related species with different lifestyles, allows for unique comparisons to be made to identify how pathogens and virulence determinants have emerged. Among several established virulence determinants, secondary metabolites (SMs) have been suggested to play significant roles during phytopathogen infection. Interestingly, the secondary metabolism of Dutch elm pathogens remains almost unexplored, and little is known about how SM biosynthetic genes are organized in these species. To better understand the metabolic potential of O. ulmi and O. novo-ulmi, we performed a deep survey and description of SM biosynthetic gene clusters (BGCs) in these species and assessed their conservation among eight species from the Ophiostomataceae family. Among 19 identified BGCs, a fujikurin-like gene cluster (OpPKS8) was unique to Dutch elm pathogens. Phylogenetic analysis revealed that orthologs for this gene cluster are widespread among phytopathogens and plant-associated fungi, suggesting that OpPKS8 may have been horizontally acquired by the Ophiostoma genus. Moreover, the detailed identification of several BGCs paves the way for future in-depth research and supports the potential impact of secondary metabolism on Ophiostoma genus’ lifestyle

    Vulnerabilidades e subjetividades de pessoas com diagnóstico e tratamento de hepatite C

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    Este estudo analisa as vulnerabilidades e subjetividades de usuários com hepatite C acompanhados por um centro de especialização. O estudo qualitativo buscou captar as complexas vulnerabilidades experimentadas por esses sujeitos. Expõem-se as formas de reação na busca pelo tratamento e no enfrentamento dos efeitos adversos da medicação, bem como as restrições impostas pela doença. As divergências no enfrentamento dependem da ressignificação das biografias e da reorganização das subjetividades. O tratamento está centralizado mais na terapia medicamentosa do que nos efeitos sobre a reorganização de suas vidas, questão que merece um olhar crítico, tendo presente a integralidade do cuidado à saúde
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