Integration Host Factor (IHF) binds to the promoter region of the phtD operon involved in phaseolotoxin synthesis in P. syringae pv. phaseolicola NPS3121

<p>Abstract</p> <p>Background</p> <p><it>Pseudomonas syringae </it>pv. phaseolicola, the causal agent of halo blight disease in beans, produces a toxin known as phaseolotoxin, in whose synthesis participate a group of genes organized within the genome in a region known as the "Pht cluster". This region, which is thought to have been acquired by horizontal gene transfer, includes 5 transcriptional units, two monocistronic (<it>argK, phtL</it>) and three polycistronic (<it>phtA, phtD, phtM</it>), whose expression is temperature dependent. So far, the regulatory mechanisms involved in phaseolotoxin synthesis have not been elucidated and the only well-established fact is the requirement of low temperatures for its synthesis. In this work, we searched for regulatory proteins that could be involved in phaseolotoxin synthesis, focusing on the regulation of the <it>phtD </it>operon.</p> <p>Results</p> <p>In this study we identified the global regulator IHF (Integration Host Factor), which binds to the promoter region of the <it>phtD </it>operon, exerting a negative effect on the expression of this operon. This is the first regulatory protein identified as part of the phaseolotoxin synthesis system. Our findings suggest that the Pht cluster was similarly regulated in the ancestral cluster by IHF or similar protein, and integrated into the global regulatory mechanism of <it>P. syringae </it>pv. phaseolicola, after the horizontal gene transfer event by using the host IHF protein.</p> <p>Conclusion</p> <p>This study identifies the IHF protein as one element involved in the regulation of phaseolotoxin synthesis in <it>P. syringae </it>pv. phaseolicola NPS3121 and provides new insights into the regulatory mechanisms involved in phaseolotoxin production.</p

Transcriptional profile of Pseudomonas syringae pv. phaseolicola NPS3121 in response to tissue extracts from a susceptible Phaseolus vulgaris L. cultivar

<p>Abstract</p> <p>Background</p> <p><it>Pseudomonas syringae </it>pv. phaseolicola is a Gram-negative plant-pathogenic bacterium that causes "halo blight" disease of beans (<it>Phaseolus vulgaris </it>L.). This disease affects both foliage and pods, and is a major problem in temperate areas of the world. Although several bacterial genes have been determined as participants in pathogenesis, the overall process still remains poorly understood, mainly because the identity and function of many of the genes are largely unknown. In this work, a genomic library of <it>P. syringae </it>pv. phaseolicola NPS3121 was constructed and PCR amplification of individual fragments was carried out in order to print a DNA microarray. This microarray was used to identify genes that are differentially expressed when bean leaf extracts, pod extracts or apoplastic fluid were added to the growth medium.</p> <p>Results</p> <p>Transcription profiles show that 224 genes were differentially expressed, the majority under the effect of bean leaf extract and apoplastic fluid. Some of the induced genes were previously known to be involved in the first stages of the bacterial-plant interaction and virulence. These include genes encoding type III secretion system proteins and genes involved in cell-wall degradation, phaseolotoxin synthesis and aerobic metabolism. On the other hand, most repressed genes were found to be involved in the uptake and metabolism of iron.</p> <p>Conclusion</p> <p>This study furthers the understanding of the mechanisms involved, responses and the metabolic adaptation that occurs during the interaction of <it>P. syringae </it>pv. phaseolicola with a susceptible host plant.</p

Lo glocal y el turismo. Nuevos paradigmas de interpretación.

El estudio del turismo se realiza desde múltiples escalas y enfoques, este libro aborda muchos temas que es necesario discutir desde diversas perspectivas; es el caso de la reflexión sobre la propia disciplina y sus conceptos, así como los asuntos específicos referidos al impacto territorial, los tipos de turismo, las cuestiones ambientales, el tema de la pobreza, la competitividad, las políticas públicas, el papel de las universidades, las áreas naturales protegidas, la sustentabilidad, la cultura, el desarrollo, la seguridad, todos temas centrales documentados y expuestos con originalidad y dominio del asunto. Lo multiescalar es básico para la comprensión del sistema turístico, sistema formado de procesos globales, regionales y locales. El eje de discusión del libro es lo glocal, esa interacción entre lo nacional y local con lo global

4to. Congreso Internacional de Ciencia, Tecnología e Innovación para la Sociedad. Memoria académica

Este volumen acoge la memoria académica de la Cuarta edición del Congreso Internacional de Ciencia, Tecnología e Innovación para la Sociedad, CITIS 2017, desarrollado entre el 29 de noviembre y el 1 de diciembre de 2017 y organizado por la Universidad Politécnica Salesiana (UPS) en su sede de Guayaquil. El Congreso ofreció un espacio para la presentación, difusión e intercambio de importantes investigaciones nacionales e internacionales ante la comunidad universitaria que se dio cita en el encuentro. El uso de herramientas tecnológicas para la gestión de los trabajos de investigación como la plataforma Open Conference Systems y la web de presentación del Congreso http://citis.blog.ups.edu.ec/, hicieron de CITIS 2017 un verdadero referente entre los congresos que se desarrollaron en el país. La preocupación de nuestra Universidad, de presentar espacios que ayuden a generar nuevos y mejores cambios en la dimensión humana y social de nuestro entorno, hace que se persiga en cada edición del evento la presentación de trabajos con calidad creciente en cuanto a su producción científica. Quienes estuvimos al frente de la organización, dejamos plasmado en estas memorias académicas el intenso y prolífico trabajo de los días de realización del Congreso Internacional de Ciencia, Tecnología e Innovación para la Sociedad al alcance de todos y todas

Multiancestry analysis of the HLA locus in Alzheimer’s and Parkinson’s diseases uncovers a shared adaptive immune response mediated by HLA-DRB1*04 subtypes

Across multiancestry groups, we analyzed Human Leukocyte Antigen (HLA) associations in over 176,000 individuals with Parkinson’s disease (PD) and Alzheimer’s disease (AD) versus controls. We demonstrate that the two diseases share the same protective association at the HLA locus. HLA-specific fine-mapping showed that hierarchical protective effects of HLA-DRB1*04 subtypes best accounted for the association, strongest with HLA-DRB1*04:04 and HLA-DRB1*04:07, and intermediary with HLA-DRB1*04:01 and HLA-DRB1*04:03. The same signal was associated with decreased neurofibrillary tangles in postmortem brains and was associated with reduced tau levels in cerebrospinal fluid and to a lower extent with increased Aβ42. Protective HLA-DRB1*04 subtypes strongly bound the aggregation-prone tau PHF6 sequence, however only when acetylated at a lysine (K311), a common posttranslational modification central to tau aggregation. An HLA-DRB1*04-mediated adaptive immune response decreases PD and AD risks, potentially by acting against tau, offering the possibility of therapeutic avenues

Agricultural biotechnology and the poor : proceedings of an international conference, Washington, D.C., 21-22 October 1999. Mexico: ensuring environmental safety while benefiting from biotechnology.

Chapter "Mexico: ensuring environmental safety while benefiting from biotechnology" from "Agricultural biotechnology and the poor : proceedings of an international conference, Washington, D.C., 21-22 October 1999"

Estimación del estado del motor de reluctancia conmutada MFR132.5 mediante Filtro Desaromatizado de Kalman

The measure of the instant angular position of the rotor is integral part of the control of Switching Reluctance Motors drivers. The direct measurement of this variable adds complexity and cost to the system and it doesn�t give good results sometimes. That has motivated the development and test of algorithms for estimating the rotor angular position of the motor. In this work is presented the use of an Unscented Kalman Filter for estimating the speed and rotor angular position of the motor MFR 132.5, based on a grey box model of the motor which is also presented in this work. The results show that this kind of estimator gives good estimations of the state of the motor, as well during the starting as during the regular operation, even under the presence of perturbations of the load torque and errors in the initial estimation of the angular position.La determinación de la posición angular instantánea del rotor es parte integral del control en los accionamientos de Motores de Reluctancia Conmutada. La medición directa de esta variable adiciona complejidad y costo al sistema y no siempre brinda buenos resultados, lo que ha motivado el desarrollo y prueba de algoritmos de estimación de la posición angular del rotor. En este trabajo se presenta el empleo de un Filtro Desaromatizado de Kalman para estimar la velocidad y la posición angular del rotor del motor MFR 132.5, basado en un modelo de caja gris de dicho motor que también es presentado en este trabajo. Los resultados demuestran que este estimador ofrece estimaciones satisfactorias del estado del motor, lo mismo durante el arranque que durante el funcionamiento regular, aún bajo la presencia de perturbaciones en el torque de la carga y errores en la estimación inicial de la posición angular del rotor

Estimación del estado del motor de reluctancia conmutada MFR132.5 mediante Filtro Desaromatizado de Kalman. State estimation of the switching reluctance motor MFR132.5 using an Unscented Kalman Filter

<p>La determinación de la posición angular instantánea del rotor es parte integral del control en los accionamientos de Motores de Reluctancia Conmutada. La medición directa de esta variable adiciona complejidad y costo al sistema y no siempre brinda buenos resultados, lo que ha motivado el desarrollo y prueba de algoritmos de estimación de la posición angular del rotor. En este trabajo se presenta el empleo de un Filtro Desaromatizado de Kalman para estimar la velocidad y la posición angular del rotor del motor MFR 132.5, basado en un modelo de caja gris de dicho motor que también es presentado en este trabajo. Los resultados demuestran que este estimador ofrece estimaciones satisfactorias del estado del motor, lo mismo durante el arranque que durante el funcionamiento regular, aún bajo la presencia de perturbaciones en el torque de la carga y errores en la estimación inicial de la posición angular del rotor.</p><p> </p><p> </p><p>The measure of the instant angular position of the rotor is integral part of the control of Switching Reluctance Motors drivers. The direct measurement of this variable adds complexity and cost to the system and it doesn’t give good results sometimes. That has motivated the development and test of algorithms for estimating the rotor angular position of the motor. In this work is presented the use of an Unscented Kalman Filter for estimating the speed and rotor angular position of the motor MFR 132.5, based on a grey box model of the motor which is also presented in this work. The results show that this kind of estimator gives good estimations of the state of the motor, as well during the starting as during the regular operation, even under the presence of perturbations of the load torque and errors in the initial estimation of the angular position.</p