Nucleoside diphosphate kinase A (NM23-H1) as a potential biomarker candidate for colorectal cancer

Comunicaciones a congreso

Proteomic approach to improve the diagnosis of malignant pleural effusions

Comunicaciones a congreso

In the search of early stages biomarkers for non small cell lung cancer

Comunicaciones a congreso

Proteomic characterization of the hemolymph of Octopus vulgaris infected by the protozoan parasite Aggregata octopiana

13 páginas, 1 tabla, 3 figurasThe immune system of cephalopods is poorly known to date. The lack of genomic information makes difficult to understand vital processes like immune defense mechanisms and their interaction with pathogens at molecular level. The common octopus Octopus vulgaris has a high economic relevance and potential for aquaculture. However, disease outbreaks provoke serious reductions in production with potentially severe economic losses. In this study, a proteomic approach is used to analyze the immune response of O. vulgaris against the coccidia Aggregata octopiana, a gastrointestinal parasite which impairs the cephalopod nutritional status. The hemocytes and plasma proteomes were compared by 2-DE between sick and healthy octopus. The identities of 12 differentially expressed spots and other 27 spots without significant alteration from hemocytes, and 5 spots from plasma, were determined by mass spectrometry analysis aided by a six reading-frame translation of an octopus hemocyte RNA-seq database and also public databases. Principal component analysis pointed to 7 proteins from hemocytes as the major contributors to the overall difference between levels of infection and so could be considered as potential biomarkers. Particularly, filamin, fascin and peroxiredoxin are highlighted because of their implication in octopus immune defense activity. From the octopus plasma, hemocyanin was identified. This work represents a first step forward in order to characterize the protein profile of O. vulgaris hemolymph, providing important information for subsequent studies of the octopus immune system at molecular level and also to the understanding of the basis of octopus tolerance-resistance to A. octopianaXunta de Galicia (10PXIB402116PR)CONACyT (Mexico)Xunta de Galicia, “I. Parga Pondal” fellowshipMinisterio de Ciencia e Innovación (BFU2011-22599)Fondos Feder “unha maneira de facer Europa” and Xunta de Galicia (“Grupos con Potencial Crecimiento GPC2013-011”)Peer reviewe

Multivariate methods aid in pinpointing promising tumor marker candidates from colorectal biopsies

The application of proteomic techniques to the search for disease markers is widely reported nowadays. However, the data rendered by these methods is highly complex and requires mining through statistical methods. Since univariate tests are prone to false positives and require post-test correction, multivariate methods seem more suitable for the task. Here we show an example of their utility, applying both principal component analysis (PCA) and linear discriminant analysis (LDA) to the hydrophobic subproteome of the colorectal mucosa. In order to find proteins specifically altered by colorectal cancer, we compared both the tumor and the adjacent healthy mucosa. PCA followed by variable selection, and corroboration by LDA, pointed out the proteins vimentin and prohibitin as promising candidates for the diagnosis of colorectal tumors.

A simple electroelution method for rapid protein purification: isolation and antibody production of alpha toxin from Clostridium septicum

Clostridium septicum produces a number of diseases in human and farm animals which, in most of the cases, are fatal without clinical intervention. Alpha toxin is an important agent and the unique lethal virulent factor produced by Clostridium septicum. This toxin is haemolytic, highly lethal and necrotizing activities but is being used as an antigen to develop animal vaccines. The aim of this study was to isolate the alpha toxin of Clostridium septicum and produce highly specific antibodies against it. In this work, we have developed a simple and efficient method for alpha toxin purification, based on electroelution that can be used as a time-saving method for purifying proteins. This technique avoids contamination by other proteins that could appear during other protein purification techniques such chromatography. The highly purified toxin was used to produce polyclonal antibodies. The specificity of the antibodies was tested by western blot and these antibodies can be applied to the quantitative determination of alpha toxin by slot blot

Shotgun proteomics applied to the study of new biomarkers in schizophrenia

Poster.-- 18th National Meeting of the Spanish Society in Neuroscience, September 4th, 2019 to September 6th, 2019, Santiago de CompostelaShotgun proteomics allow the study of the complete proteome without the need to work with isolated proteins. In this techniques, from the enzymatic digestion of the proteins contained in a biological sample can be carried out a massive analysis of the peptides obtained by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). Because this technique allows a proteomic analysis from a wide range of biological samples, shotgun proteomics turns out to be highly interesting in the identification of biomarkers of mental disorders like Schizophrenia (SZ), where the affected tissue cannot be analysed in vivo and in which case the study is made from biological fluids such as bloodThis work was supported by a Ramón & Cajal grant (RYC-2014-15246) and by Galicia Innovation Agency-GAIN grant (IN607D-2016/003; IN607D-2017/01).Peer reviewe