Analysis of the evolution of a fisheries management plan based on environmental governance: living laboratory in the Olo River, Portugal

Environmental governance aims to develop and understand the lessons, achievements and challenges of planning at both local and global levels. In particular, integrated river management is key to the sustainable development of ecosystems, and the collaborative and inclusive approach among stakeholders supports the decision-making process. The objective of this study was to assess the current management model for recreational fisheries in the Olo River Reserved Fishing Zone (RFZ). This management model aims to better rationalise the use of fishery resources by allowing only recreational fishing, ensuring the sustainability of this activity and of the environment, and guaranteeing that this activity contributes to the development of the basin. To this end, the state of this activity was evaluated before and after the implementation of the First Management Plan and the Second Management Plan. Samples of ichthyofauna were taken and an analysis of the data on the age, growth and physical condition of the endemic species was carried out. Multivariate analysis techniques applied to the biological and physical data sets allowed the determination of distribution patterns of Olo Basin and the relative importance of environmental variables in the discrimination of the fish assemblages.The presence of the species Salmo trutta fario improved in the stations sampled throughout the study. The abundance of fish throughout the study showed a remarkable improvement, from 1229 (year 2008), 1142 (year 2013) and 1781 (year 2018), the drop in 2013 was being due to a wildfire in the area. This fishing area has been a true living laboratory for the development of new and more sustainable inland fisheries management practices. The development of this planning and management model was successfully exported to other river basins in the area

Possibilities for exploitation of invasive species, Arundo donax L., as a source of phenol compounds

Recycling and reuse are a fundamental pillar for reducing global pollution. In this context, the use of waste generated by invasive species control actions, as a natural raw material for the exploitation of their bioactive compounds, provides a great interest for a more sustainable industry. In this study, phenolic compounds were determined from the different parts that compose the invasive species Arundo donax L. The experiments were carried out using response surface methodology (RSM) by the Box-Behnken method, allowing us to evaluate the factors (temperature, time and type of solvent) and the values that optimized the extraction. The highest concentration of total phenolic compounds equivalent in gallic acid (GAE) was obtained from extracts with distilled water, with the fraction of leaves (7.43 mg GAE /g) in first place, followed by inflorescences (5.15 mg GAE /g) and finally stems (2.73 mg GAE /g).These optimum values were obtained using conditions of 6 h and 45 °C for leaves and stems and 4 h and 35 °C for inflorescences.. Identification and quantification of phenolic acids and flavonoids were carried out by HPLC–MS/MS analytical technique. In general, stem extracts were the most abundant in phenolic acids with potential pharmacological properties. These studies provided promising results for the Arundo donax L. species as a source of polyphenols, making full use of its residue and aiming to boost the circular economy in important industry sectors.Financiado para publicación en acceso aberto: Universidade de Vigo/CISU

Anthocyanins and total phenolic compounds from pigment extractions of non-native species from the Umia river basin: Eucalyptus globulus, Tradescantia fluminensis, and Arundo donax

Alien invasive species (AIS) and non-native species are a prominent and extended problem in a wide range of areas in Europe and around the world. Centered in the Umia’s riparian forest, in Galicia, we found at least three main AIS needing to be controlled and harvested to preserve the biodiversity of the area. Previous studies probed that leaves and bark of selected species—Tradescantia fluminensis, Arundo donax, and Eucalyptus globulus—have important antioxidant properties, suitable for use in pharmaceutical and industrial contexts. A comparison of four solid-liquid extraction methods—Soxhlet extraction, ultrasound assisted extraction, thermal agitator, and infusion—was conducted in order to define the most efficient method in correlation within antioxidant (anthocyanins and total phenols) extraction. Water was selected as solvent, providing a sustainable research background without implying any chemical additives. The best extraction yields were obtained with Soxhlet extraction for all raw matter, with best results for Tradescantia fluminensis (41.89%) and Eucalyptus globulus leaves (39.35%); followed by the ultrasonic assisted extraction method, with better yield performed with Eucalyptus leaves (27.07%). On the contrary, Tradescantia fluminensis showed better efficiency with thermal agitator (35.07% compared to 23.19% from ultrasound extraction). After extractions, identification and quantification of total phenolic compounds and anthocyanins were carried out using spectrophotometric determination and acid hydrolysis in butanol method. In general, the best extraction yield in correlation with higher antioxidant content was performed by thermal agitator method, and Eucalyptus globulus leaves were demonstrated to be the better anthocyanins (6.18 ± 0.82 mg CC/g) and total phenols containers (172.40 ± 44.53 mg GAE/g). Studies provided promising possibilities for the residues of the three non-native species analyzed, as a source of antioxidants, favoring circular economy systems, as well as taking care of biodiversity in affected environments.Xunta de Galicia | Ref. Becas predoctorales 29

PLS-PM analysis of forest fires using remote sensing tools. The case of Xurés in the Transboundary Biosphere Reserve

Forest fires have environmental, social and economic impacts in many areas. Various factors related to territory directly influence both the number and the surface area of each fire. The link between different variables (climate, social and environmental) in the risk of fire and in the characteristics of fires is studied here through Partial Least Squares - Path Models. In addition, images from the Sentinel-2 sensor and geographic information systems are used to create a cartographic base of fires in the Transboundary Biosphere Reserve of Galicia and the Site of Community Importance of Xurés (Galicia) between 2015 and 2020. In all, seven variables are analyzed in this study area using the partial least squares-path modeling method: climate, topography, land use, type of environmental protection, the anthropogenic factor, fire defense, and fire data (severity and area). The parameters for each variable are used to obtain weights and thus determine the importance of each one. The areas where the problem of forest fires is greatest are those with the greatest environmental protection. Up to 31% of the surface area of the Natura 2000 Network was burned in the 6-year study period. Topography and land use are also shown to be relevant factors in the effects of forest fires in this territory. By contrast, higher population density and the development of infrastructures such as roads and water tanks mitigate the impact of fires. The problem of forest fires encompasses many variables that need to be studied. By contextualizing each study area as far as possible, specific measures to prevent and reduce damage can be drawn up.Agencia Estatal de Investigación | Ref. PCI2020–120705-2Financiado para publicación en acceso aberto: Universidade de Vigo/CISU

Thalassiosira pseudonana and Skeletonema costatum biomass optimization: cultivation, harvesting, extraction of oils and biodiesel and pelletization of the residue

Financiado para publicación en acceso aberto: Universidade de Vigo/CISUGMicroalgae are one of the most promising feedstocks for biofuel production that can solve the energy crisis, climate change, and the depletion of fossil fuels. Biorefineries have production capacity bottlenecks that prevent them from being economically profitable, without leaving aside the environmental safety of by-products. This research aims to analyze critical stages such as harvesting or lipid extraction from two microalgae species currently unknown, such as Thalassiosira pseudonana and Skeletonema costatum. Inorganic flocculation with a low concentration of iron or aluminum salts (FeCl3 and Al2(SO4)3) was achieved to recover >60% biomass in just 20 min in both cases. Lipids extractions through chloroform: methanol (solvent ratio 2:1) obtained low performance due to the ionic strength medium. The fatty acid composition of the algae extracts showed that stearic acid (C18:0) and palmitoleic acid (C16:1) were predominant in both species. In addition, residues from the lipid extraction process were used for the manufacture of pellets. The data collected showed that these solid biofuels should be combined with other biomass typologies if the end-use are biomass boilers. The development of these studies provides new information on different microalgae species and their potential to use their biomass through an integrated utilization

Modelling and evaluation of land use changes through satellite images in a multifunctional catchment: social, economic and environmental implications

Financiado para publicación en acceso aberto: Universidade de Vigo/CISUGFloods are recurrent phenomena with significant environmental and socio-economic impacts. The risk of flooding increases when land use changes. The objective of this research is to detect land cover changes via Sentinel-2 images in the Umia Basin (Galicia, NW Spain) in 2016–2021 and to analyse the associated flood risk. This study focuses on how forest use and nature-based solutions (NBS) can reduce the risk and hazard of flooding in cities and crops in the high-risk area. A flood simulation was performed with the land use obtained from Sentinel-2 (Observed) and three more simulations were performed changing the location of afforestation and NBS, i.e. “S-Upstream”, “S-Downstream” and “S-Total”. Finally, the environmental, economic and social impacts of the scenarios designed and estimated are analysed and discussed. Land cover change was successfully monitored with Sentinel-2 imagery. The catchment area showed noteworthy changes in land use, most notably for the category of trees, which covered 6700 ha in 2016 and 10,911 ha in 2021. However riparian vegetation decreased by almost 11%. For the flood hazard simulations, an average reduction in peak discharge was obtained for all three scenarios (9.3% for S-Up; 8.6% for S-Down and 13% for S-Total). From the economic perspective, all three scenarios show a positive net present value for the period studied. However, S-Down is the scenario with the lowest benefits (€15,476,487), while S-Up and S-Total show better values at €29,580,643 and €65,158,130 respectively. However, investment cost is much higher for the S-Total scenario, and upstream actions affect the whole catchment, so S-Up is the best decision. This study concludes that the information provided by satellites is a large-scale analysis tool for small heterogeneous plots that facilitates the comprehensive analysis of a territory. This information can be incorporated into flood analysis models, facilitating simulation through the use of NBS. It has been proven that the use of reforestation upstream only is almost as beneficial as reforestation in the entire catchment and is economically more viable. This confirms that the methodology used reduces flood hazard, despite the territorial complexity, facilitating decision making on the use of NBS.Universidade de Vig

Assessment of high spatial resolution satellite imagery for monitoring riparian vegetation: riverine management in the smallholding

Riverine habitats are essential ecotones that bridge aquatic and terrestrial ecosystems, providing multiple ecosystem services. This study analyses the potential use of high-resolution satellite imagery, provided by the WorldView-2 satellite, in order to assess its viability for monitoring riparian ecosystems. It is performed by calculating the riparian strip quality index (RSQI) and calibrating it with the riparian quality index (QBR). The methodology was implemented in the Umia River, which is characterised by elevated anthropogenic pressures (located in the northwest of Spain). The results obtained by the method have a 92% of veracity and a kappa coefficient of 0.88. The average quality value obtained for the RSQI index was 71.57, while the average value for the QBR was 55.88. This difference could be attributed to the fact that the former does not differ between autochthonous and non-autochthonous vegetation. The areas with more accurate mapping corresponded to stretches of vegetation with optimal cover (80–50%), with good connectivity with the adjacent forest ecosystem and few or no presence of invasive plants. The worst-scoring sites had the next characteristics: low connectivity (< 10%), low forest cover (< 10%) and a higher presence of invasive plants. The degradation of vegetation could be explained by the presence of agriculture and deficient land use rationing caused by the type of ownership of the study area. The application of this index through satellite images will facilitate the environmental governance of multiple ecosystems and in special riparian ecosystems, obtaining a quick and objective methodology, easily replicable in other basins.Universidade de Vigo/CISUGXunta de Galicia | Ref. ED431B 2022/1

Drivers and trends in the size and severity of forest fires endangering WUI areas: a regional case study

[EN] This study explored, for the first time, the drivers shaping large fire size and high severity of forest fires classified as level-2 in Spain, which pose a great danger to the wildland–urban interface. Specifically, we examined how bottom-up (fuel type and topography) and top-down (fire weather) controls shaped level-2 fire behavior through a Random Forest classifier at the regional scale in Galicia (NW Spain). We selected for this purpose 93 level-2 forest fires. The accuracy of the RF fire size and severity classifications was remarkably high (>80%). Fire weather overwhelmed bottom-up controls in controlling the fire size of level-2 forest fires. The likelihood of large level-2 forest fires increased sharply with the fire weather index, but plateaued at values above 40. Fire size strongly responded to minimum relative humidity at values below 30%. The most important variables explaining fire severity in level-2 forest fires were the same as in the fire size, as well as the pre-fire shrubland fraction. The high-fire-severity likelihood of level-2 forest fires increased exponentially for shrubland fractions in the landscape above 50%. Our results suggest that level-2 forest fires will pose an increasing danger to people and their property under predicted scenarios of extreme weather conditionsSIProject 4Map4Health is selected in the call ERA-Net CHIST-ERA (2019) and funded by the State Research Agency of Spain (reference PCI2020-120705-2/AEI/10.13039/501100011033). PMF and JMFG were supported by National Funds from FCT—Portuguese Foundation for Science and Technology, under the project UIDB/04033/2020. JMFG was supported by a postdoctoral fellowship granted by the Ramón Areces Foundatio

Influence of Microcystis sp. and freshwater algae on pH: changes in their growth associated with sediment

Samples from two reservoirs with eutrophication problems, located in Pontevedra and Ourense (Northwestern Spain), were cultured, along with a third crop from a reservoir with no problems detected in Ourense (Northwestern Spain). The samples were grown under the same conditions (with an average temperature of 21 ± 2 °C, and a 3000 lux light intensity) in triplicate, and their growth, absorbance and pH were studied. High correlation values were obtained for pH and cellular growth (R2 ≥ 95%). The water from Salas showed the greatest microalgal growth (0.15 × 106 cells/ml to 31.70 × 106 cells/ml of "Microcystis sp." for the last day of culturing) and the greatest increase in pH (5.72–9.02). In all the cultures studied here, the main species that reproduced was "Microcystis sp.", which can produce neurotoxins and hepatotoxins. In addition, water samples were cultured with sediments of their own reservoir and with others to observe their evolution. The sediments studied in this case were rich in biotites, which can lead phosphate to be a limiting factor for phytoplankton due to the formation and sedimentation of insoluble salts of ferric phosphate. In crops grown with sediments from the Salas reservoir, actinobacteria developed which can inhibit microalgal growth. The study of the growth of cyanobacteria and possible methods of inhibiting them directly concerns the quality of water and its ecosystems, avoiding pollution and impact on ecosystems

Water toxicity in reservoirs after freshwater algae harvest

Blooms of microalgae and cyanobacteria increase every year, presenting great problems for the environment. Finding a way of harvesting these microalgae could be useful for water governance. Furthermore, the method should not cause cell lysis and should thus avoiding discharging toxins into the water. Three reservoirs were studied, two of them with eutrophication problems (As Conchas and A Baxe) and another (Salas) with no such problems. Three different harvesting methods were studied; electroflocculation (EF) with the application time being varied; centrifugation, with application times and speeds being varied; and finally, natural sedimentation. The highest efficiency was obtained in the culture from A Baxe, which had a higher initial absorbance value (1.664), using EF (90.64% for an application time of 2 min and 30 s) and centrifugation at 4000 rpm (92.25% for2 minutes, 92.73% for 5 min). Electrofloculation can obtain up to 84% more biomass than natural sedimentation alone. Sample toxicity was studied before and after harvesting using Microcystest and found to be higher after harvesting. It was observed that for the same sample, the higher the yield was the greater the toxicity was. For the A Baxe culture with an application time of 2 min, a speed of 2000 rpm and a yield of 87.02%, a toxicity figure of 0.94 μg/L was obtained, while for a speed of 4000 rpm the yield was 92.25% and the toxicity was 1.05 μg/L. The toxicity limit set by the World Health Organization (WHO) is 1 μg/L, and this small difference seems to be key. With these results, this study concludes that chlorophyll levels may interfere with the test used. Future tests or analyses should be developed so as to avoid such interference, which may alter the toxin values. Electroflocculation seems to be a promising method since it does not cause the lysis of "Microcystis aeruginosa", whereas the centrifugation method could give problems. Finally, it is worth highlighting the importance of performing toxin measurements after harvesting the microalgae to check that the method is viable in natural ecosystems