Guidelines for the use of cell lines in biomedical research

Cell-line misidentification and contamination with microorganisms, such as mycoplasma, together with instability, both genetic and phenotypic, are among the problems that continue to affect cell culture. Many of these problems are avoidable with the necessary foresight, and these Guidelines have been prepared to provide those new to the field and others engaged in teaching and instruction with the information necessary to increase their awareness of the problems and to enable them to deal with them effectively. The Guidelines cover areas such as development, acquisition, authentication, cryopreservation, transfer of cell lines between laboratories, microbial contamination, characterisation, instability and misidentification. Advice is also given on complying with current legal and ethical requirements when deriving cell lines from human and animal tissues, the selection and maintenance of equipment and how to deal with problems that may arise

Environmental policy instruments in a new era

The aim of this paper is to give a long term sustainability perspective on instrumentation in environmental policy, within a broad, also strategic, evaluative framework. To arrive at integrated insight, the basic function of policy instruments is discussed: why do you need them at all and how would they look like? It comes out that it is not at all clear how policy instruments can be classified and described. Nor is it clear how a consistent evaluation of policy instruments can be set up. Still, as some ordering is necessary for instrument development and instrument choice, an analytic framework is developed. One basic problem in discussions on policy instruments is that both their functioning and their effects are context dependent. This implies that in decisions on policy instrumentation, binding society for a long time, also long term changes in context are to be taken into account, in terms of structural, cultural and economic developments in society. Some main lines of development are discussed, with implications for instrument choice. Within these mouldable boundaries, some practical guidelines are given for policy development at a case level. --environmental policy,environmental strategy,policy instruments,policy evaluation,globalisation

SOFTWARE INTEROPERABILITY: Issues at the Intersection between Intellectual Property and Competition Policy

The dissertation project proceeds through three papers, analyzing issues related to software interoperability and respectively pertaining to one of the three following interdependent levels of analysis. The first level addresses the legal status of software interoperability information under current intellectual property law (focusing on copyright law, which is the main legal tool for the protection of these pieces of code), trying to clarify if, how and to what extent theses pieces of code (and the associated pieces of information) are protected erga omnes by the law. The second level complements the first one, analyzing legal and economic issues related to the technical possibility of actually accessing this interoperability information through reverse engineering (and software decompilation in particular). Once a de facto standard gains the favor of the market, reverse engineering is the main self-help tool available to competitors in order to achieve interoperability and compete “inside this standard”. The third step consists in recognizing that – in a limited number of cases, but which are potentially of great economic relevance – market failures could arise, despite any care taken in devising checks and balances in the legal setting concerning both the legal status of interoperability information and the legal rules governing software reverse engineering. When this is the case, some undertakings may stably gain a dominant position in software markets, and possibly abuse it. Hence, at this level of analysis, competition policy intervention is taken into account. The first paper of the present dissertation shows that interoperability specifications are not protected by copyright. In the paper, I argue that existing doubts and uncertainty are typically related to a poor understanding of the technical nature of software interfaces. To remedy such misunderstanding, the paper focuses on the distinction between interface specifications and implementations and stresses the difference between the steps needed to access to the ideas and principle constituting an interfaces specification and the re-implementation of a functionally equivalent interface through new software code. At the normative level, the paper shows that no major modifications to the existing model of legal protection of software (and software interfaces) are needed; however, it suggests that policymakers could reduce the Fear of legal actions, other forms of legal Uncertainty and several residual Doubts (FUD) by explicitly stating that interface specifications are unprotectable and freely appropriable. In the second paper, I offer a critique of legal restraints on software reverse engineering, focusing in particular on Europe, but considering also similar restraints in the US, in particular in the context of the Digital Millennium Copyright Act. Through an analysis of entry conditions for late comers and of the comparative costs of developing programs in the first place or reverse engineering them, the paper shows that limitations on decompilation imposed by article 6 of the Software Directive were mostly superfluous and basically non-binding at the time of drafting. What is more, the paper shows that nowadays new – and largely unanticipated – developments in software development models (e.g. open source) make these restraints an obstacle to competition against dominant incumbent controlling software platforms. In fact, limitations on the freedom to decompile obstacle major reverse engineering projects performed in a decentralized way, as in the context of an open source community. Hence, since open source projects are the most credible tools to recreate some competitive pressure in a number of crucial software markets, the paper recommends creating a simpler and clear-cut safe harbor for software reverse engineering. The third paper claims that, in software markets, refusal-to-deal (or “information-withholding”) strategies are normally complementary with tying (or “predatory-innovation”) strategies, and that this complementarity is so relevant that dominant platform controllers need to couple both in order to create significant anti- competitive effects. Hence, the paper argues that mandatory unbundling (i.e. mandating a certain degree of modularity in software development) could be an appropriate – and frequently preferable – alternative to mandatory disclosure of interoperability information. However, considering the critiques moved from part of the literature to the Commission’s Decision in the recent European Microsoft antitrust case, an objection to the previous argument could be that – also in the case of mandatory unbundling – one should still determine the minimum price for the unbundled product. The last part of the paper applies some intuitions coming from the literature concerning complementary oligopoly to demonstrate that this objection is not well grounded and that – in software markets – mandatory unbundling (modularity) may be a useful policy even if the only constraint on the price of the unbundled good is the one of non-negativity

Towards an\u2028 EU research and innovation policy agenda for nature-based solutions & re-naturing cities. Final report of the Horizon 2020 expert group on nature-based solutions and re-naturing cities.

1. Nature-based solutions harness the power and sophistication of nature to turn environmental, social and economic challenges into innovation opportunities. They can address a variety of societal challenges in sustainable ways, with the potential to contribute to green growth, 'future-proofing' society, fostering citizen well-being, providing business opportunities and positioning Europe as a leader in world markets. \u2028 2. Nature-based solutions are actions which are inspired by, supported by or copied from nature. They have tremendous potential to be energy and resource-efficient and resilient to change, but to be successful they must be adapted to local conditions. \u2028 3. Many nature-based solutions result in multiple co-benefits for health, the economy, society and the environment, and thus they can represent more efficient and cost-effective solutions than more traditional approaches. \u2028 4. An EU Research & Innovation (R&I) agenda on nature-based solutions will enable Europe to become a world leader both in R&I and in the growing market for nature-based solutions. For this, the evidence base for the effectiveness of nature-based solutions needs to be developed and then used to implement solutions. Both need to be done in conjunction with stakeholders. The potential for transferability and upscaling of solutions also requires further investigation. There is also a need to develop a systemic approach that combines technical, business, finance, governance, regulatory and social innovation. \u2028 5. Four principal goals have been identified that can be addressed by nature-based solutions: �� Enhancing sustainable urbanisation through nature-based solutions can stimulate economic growth as well as improving the environment, making cities more attractive, and enhancing human well-being. \u2028 �� Restoring degraded ecosystems using nature-based solutions can improve the resilience of ecosystems, enabling them to deliver vital ecosystem services and also to meet other societal challenges. \u2028 �� Developing climate change adaptation and mitigation using nature-based solutions can provide more resilient responses and enhance the storage of carbon. \u2028 �� Improving risk management and resilience using nature-based solutions can lead to greater benefits than conventional methods and offer synergies in reducing multiple risks. \u2028 6. Based on the four goals, seven nature-based solutions for R&I actions are recommended to be taken forward by the European Commission and Member States: �� Urban regeneration through nature-based solutions \u2028 �� Nature-based solutions for improving well-being in urban areas \u2028 �� Establishing nature-based solutions for coastal resilience \u2028 �� Multi-functional nature-based watershed management and ecosystem restoration \u2028 �� Nature-based solutions for increasing the sustainability of the use of matter and energy \u2028 �� Nature-based solutions for enhancing the insurance value of ecosystems \u2028 �� Increasing carbon sequestration through nature-based solutions \u2028This report was produced by the Horizon 2020 Expert Group on 'Nature-Based Solutions and Re- Naturing Cities', informed by the findings of an e-consultation and a stakeholder workshop. \u202

Welfare Polls: A Synthesis

"Welfare polls" are survey instruments that seek to quantify the determinants of human well-being. Currently, three "welfare polling" formats are dominant: contingent-valuation surveys, QALY surveys, and happiness surveys. Each format has generated a large, specialized, scholarly literature, but no comprehensive discussion of welfare polling as a general enterprise exists. This Article seeks to fill that gap. Part I describes the trio of existing formats. Part II discusses the actual and potential uses of welfare polls in government decision making. Part III analyzes in detail the obstacles that welfare polls must overcome to provide useful well-being information, and concludes that they can be genuinely informative. Part IV synthesizes the case for welfare polls, arguing against two types of challenges: the revealed-preference tradition in economics, which insists on using behavior rather than surveys to learn about well-being; and the civic-republican tradition in political theory, which accepts surveys but insists that respondents should be asked to take a "citizen", rather than "consumer" perspective. Part V suggests new directions for welfare polls.

Environmental policy instruments in a new era

"The aim of this paper is to give a long term sustainability perspective on instrumentation in environmental policy, within a broad, also strategic, evaluative framework. To arrive at integrated insight, the basic function of policy instruments is discussed: why do you need them at all and how would they look like? It comes out that it is not at all clear how policy instruments can be classified and described. Nor is it clear how a consistent evaluation of policy instruments can be set up. Still, as some ordering is necessary for instrument development and instrument choice, an analytic framework is developed. One basic problem in discussions on policy instruments is that both their functioning and their effects are context dependent. This implies that in decisions on policy instrumentation, binding society for a long time, also long term changes in context are to be taken into account, in terms of structural, cultural and economic developments in society. Some main lines of development are discussed, with implications for instrument choice. Within these mouldable boundaries, some practical guidelines are given for policy development at a case level." (author's abstract)"Ziel dieser Studie ist es, eine Langfristperspektive für die weitere Instrumentierung der Umweltpolitik zu liefern. Dazu werden zunächst die Grundfunktionen politischer Instrumente diskutiert. Es zeigt sich, daß es keineswegs klar ist, wie man solche Instrumente kategorisieren und klassifizieren kann. Deshalb wird hierzu ein eigenes, integratives Rahmenwerk entwickelt. Der Funktionsweise und der Wirkung umweltpolitischer Instrumente gilt dann das besondere Augenmerk. Es entsteht so die bisher wohl umfassenste Darstellung und Funktionsbetrachtung des zukünftig möglichen umweltpolitischen Instrumentariums." (Autorenreferat

TECHNOLOGY ASSESSMENT FOR SUSTAINABILITY IN WATER USE. OPERATIONALIZATION OF A RESPONSIBLE GOVERNANCE BASED IN RESPONSIBLE RESEARCH AND INNOVATION (ANTICIPATION AND INCLUSIVENESS)

The management of sustainability in water resources has underscored the critical importance of determining appropriate decision-making processes and establishing effective governance structures. Gaining comprehensive insights into the decision-making mechanisms and actors involved is pivotal for tackling present as well as prospective issues related to water efficiently. This research evaluates the interplay among water scarcity, responsible technologies for water use, and systems of governance for sustainability amid swift technological progress. Furthermore, it delves into the congruity of said endeavors with the Sustainable Development Goals (SDGs), other sustainability water frameworks and the social and political ecosystem. In this context, the active engagement and participation of societal actors, and not only stakeholders, assume a pivotal role as it significantly impacts the decision-making processes and molds the results of sustainability initiatives. An innovative approach to the concepts of responsibility and sustainability is predicated on the quality of the relationship between the network of societal actors as a key point. This work underscores the importance of establishing strong and comprehensive relationships to address the challenges concerning water management and promote the adoption of sustainable approaches, in co-creation, not only of knowledge but the epistemic subject in the process. This work sheds light on the interrelated domains of water management, sustainability, and regulation. A novel proposal is presented via a simulation exercise and use the socio-technical framework for the purpose of fostering responsible water use. The comprehension and use of responsible technology and innovation in the realm of water u management will be enhanced through the technique of operationalizing open anticipatory governance and executing a simulated experiment. By using a digital deliberation space and establishing a systematic approach towards technology assessment and sustainability, using the relational quality of the network of actors as the key element for co-production of knowledge, science and technology, the present study has produced and materialized an innovative framework.Na sustentabilidade da gestão da água reveste-se de especial importância determinar processos de tomada de decisão adequados e estabelecer estruturas de governação eficazes. Obter uma visão abrangente sobre os mecanismos de tomada de decisão e os atores envolvidos é fundamental para abordar questões presentes e futuras relacionadas ao uso eficiente da água. Este trabalho procura conhecer a interação entre gestão de água, tecnologias responsáveis pelo uso da água e sistemas de governança para a sustentabilidade. Adicionalmente, pretende conhecer a relação com os Sustainable Development Goals (SDGs), outros programas de sustentabilidade, bem como com o ecossistema social e político. Neste contexto, o envolvimento e a participação ativa dos atores sociais, e não apenas de stakeholders, assume um papel fundamental, uma vez que, não só, impactam significativamente os processos de tomada de decisão, mas, também, moldam os resultados das iniciativas de sustentabilidade. Nesta nova aproximação ao conceito de responsabilidade e sustentabilidade encontra-se a qualidade da relação entre a rede de atores sociais como ponto-chave. Sublinha-se a importância de estabelecer uma qualidade relacional enriquecida e abrangente para enfrentar de forma mais estruturada os desafios relativos à gestão da água de forma eficiente e promover a adoção de abordagens sustentáveis. Com este trabalho, procura-se aprofundar os domínios inter-relacionados da gestão da água, sustentabilidade e regulamentação. É elaborada uma proposta de simulação, utilizando uma perspetiva sociotécnica com o objetivo de capacitar a co-constituição como sujeitos e a compreensão e utilização de tecnologia responsável e inovação no âmbito da gestão do uso da água utilizando operacionalização da governação antecipatória aberta. O presente estudo materializa seu carácter de inovação ao utilizar um espaço de deliberação digital e ao estabelecer uma abordagem sistemática para a avaliação da tecnologia e sustentabilidade, usando a qualidade relacional da rede de atores como elemento-chave para a coprodução de conhecimento, ciência e tecnologia e co-constituição do próprio sujeito no processo de deliberação

Assessment of Disused Public Buildings: Strategies and Tools for Reuse of Healthcare Structures

The aim of this paper, in line with the 2030 European Agenda and 2021 Italian “Recovery and Resilience Plan” objectives, is to define an evaluation methodology and tool to support public administrations to detect buildings, currently unused or underused, that might be apt for transformation interventions. The focus is on historical Italian healthcare buildings since these show widespread decay and neglect. A five-step methodology has been developed: Screening of public assets; classification, evaluation, and identification of buildings, based on the “potential index”; GIS mapping and inventory; selection of buildings for in-depth analysis; BIM digitization and definition of the “transformability index”. In the fifth phase, an evaluation tool is integrated into the BIM software to automatically calculate the transformability index of each building using six indicators: Usability, fragmentation, modifiability, roof implementation, external envelope, and window-to-wall ratio. The “transformability index” helps define the most appropriate buildings to intervene with for reuse. Building transformation is connected to construction features and layout organization and it is limited by architectural, structural, and artistic constraints