9,381 research outputs found
High-speed in vitro intensity diffraction tomography
We demonstrate a label-free, scan-free intensity diffraction tomography technique utilizing annular illumination (aIDT) to rapidly characterize large-volume three-dimensional (3-D) refractive index distributions in vitro. By optimally matching the illumination geometry to the microscope pupil, our technique reduces the data requirement by 60 times to achieve high-speed 10-Hz volume rates. Using eight intensity images, we recover volumes of ∼350 μm  ×  100 μm  ×  20  μm, with near diffraction-limited lateral resolution of   ∼  487  nm and axial resolution of   ∼  3.4  μm. The attained large volume rate and high-resolution enable 3-D quantitative phase imaging of complex living biological samples across multiple length scales. We demonstrate aIDT’s capabilities on unicellular diatom microalgae, epithelial buccal cell clusters with native bacteria, and live Caenorhabditis elegans specimens. Within these samples, we recover macroscale cellular structures, subcellular organelles, and dynamic micro-organism tissues with minimal motion artifacts. Quantifying such features has significant utility in oncology, immunology, and cellular pathophysiology, where these morphological features are evaluated for changes in the presence of disease, parasites, and new drug treatments. Finally, we simulate the aIDT system to highlight the accuracy and sensitivity of the proposed technique. aIDT shows promise as a powerful high-speed, label-free computational microscopy approach for applications where natural imaging is required to evaluate environmental effects on a sample in real time.https://arxiv.org/abs/1904.06004Accepted manuscrip
Four-dimensional tomographic reconstruction by time domain decomposition
Since the beginnings of tomography, the requirement that the sample does not
change during the acquisition of one tomographic rotation is unchanged. We
derived and successfully implemented a tomographic reconstruction method which
relaxes this decades-old requirement of static samples. In the presented
method, dynamic tomographic data sets are decomposed in the temporal domain
using basis functions and deploying an L1 regularization technique where the
penalty factor is taken for spatial and temporal derivatives. We implemented
the iterative algorithm for solving the regularization problem on modern GPU
systems to demonstrate its practical use
Deep learning in computational microscopy
We propose to use deep convolutional neural networks (DCNNs) to perform 2D and 3D computational imaging. Specifically, we investigate three different applications. We first try to solve the 3D inverse scattering problem based on learning a huge number of training target and speckle pairs. We also demonstrate a new DCNN architecture to perform Fourier ptychographic Microscopy (FPM) reconstruction, which achieves high-resolution phase recovery with considerably less data than standard FPM. Finally, we employ DCNN models that can predict focused 2D fluorescent microscopic images from blurred images captured at overfocused or underfocused planes.Published versio
TomograPy: A Fast, Instrument-Independent, Solar Tomography Software
Solar tomography has progressed rapidly in recent years thanks to the
development of robust algorithms and the availability of more powerful
computers. It can today provide crucial insights in solving issues related to
the line-of-sight integration present in the data of solar imagers and
coronagraphs. However, there remain challenges such as the increase of the
available volume of data, the handling of the temporal evolution of the
observed structures, and the heterogeneity of the data in multi-spacecraft
studies.
We present a generic software package that can perform fast tomographic
inversions that scales linearly with the number of measurements, linearly with
the length of the reconstruction cube (and not the number of voxels) and
linearly with the number of cores and can use data from different sources and
with a variety of physical models: TomograPy
(http://nbarbey.github.com/TomograPy/), an open-source software freely
available on the Python Package Index. For performance, TomograPy uses a
parallelized-projection algorithm. It relies on the World Coordinate System
standard to manage various data sources. A variety of inversion algorithms are
provided to perform the tomographic-map estimation. A test suite is provided
along with the code to ensure software quality. Since it makes use of the
Siddon algorithm it is restricted to rectangular parallelepiped voxels but the
spherical geometry of the corona can be handled through proper use of priors.
We describe the main features of the code and show three practical examples
of multi-spacecraft tomographic inversions using STEREO/EUVI and STEREO/COR1
data. Static and smoothly varying temporal evolution models are presented.Comment: 21 pages, 6 figures, 5 table
Nanometer-scale Tomographic Reconstruction of 3D Electrostatic Potentials in GaAs/AlGaAs Core-Shell Nanowires
We report on the development of Electron Holographic Tomography towards a
versatile potential measurement technique, overcoming several limitations, such
as a limited tilt range, previously hampering a reproducible and accurate
electrostatic potential reconstruction in three dimensions. Most notably,
tomographic reconstruction is performed on optimally sampled polar grids taking
into account symmetry and other spatial constraints of the nanostructure.
Furthermore, holographic tilt series acquisition and alignment have been
automated and adapted to three dimensions. We demonstrate 6 nm spatial and 0.2
V signal resolution by reconstructing various, previously hidden, potential
details of a GaAs/AlGaAs core-shell nanowire. The improved tomographic
reconstruction opens pathways towards the detection of minute potentials in
nanostructures and an increase in speed and accuracy in related techniques such
as X-ray tomography
Fast tomographic inspection of cylindrical objects
This paper presents a method for improved analysis of objects with an axial
symmetry using X-ray Computed Tomography (CT). Cylindrical coordinates about an
axis fixed to the object form the most natural base to check certain
characteristics of objects that contain such symmetry, as often occurs with
industrial parts. The sampling grid corresponds with the object, allowing for
down-sampling hence reducing the reconstruction time. This is necessary for
in-line applications and fast quality inspection. With algebraic reconstruction
it permits the use of a pre-computed initial volume perfectly suited to fit a
series of scans where same-type objects can have different positions and
orientations, as often encountered in an industrial setting. Weighted
back-projection can also be included when some regions are more likely subject
to change, to improve stability. Building on a Cartesian grid reconstruction
code, the feasibility of reusing the existing ray-tracers is checked against
other researches in the same field.Comment: 13 pages, 13 figures. submitted to Journal Of Nondestructive
Evaluation (https://www.springer.com/journal/10921
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