8,268 research outputs found
MpTCP1 controls cell proliferation and redox processes in Marchantia polymorpha
TCP transcription factors are key regulators of angiosperm cell proliferation processes. It is unknown whether their regulatory growth capacities are conserved across land plants, which we examined in liverworts, one of the earliest diverging land plant lineages. We generated knockout mutants for MpTCP1, the single TCP‐P clade gene in Marchantia polymorpha, and characterized its function conducting cell proliferation and morphological analyses as well as mRNA expression, transcriptome, chemical and DNA binding studies. Mptcp1ge lines show a reduced vegetative thallus growth and extra tissue formation in female reproductive structures. Additionally, mutant plants reveal increased H2O2 levels and an enhanced pigmentation in the thallus caused by formation of secondary metabolites, such as aminochromes. MpTCP1 proteins interact redox‐dependently with DNA and regulate the expression of a comprehensive redox network, comprising enzymes involved in H2O2 metabolism. MpTCP1 regulates Marchantia growth context‐dependently. Redox sensitivity of the DNA binding capacity of MpTCP1 proteins provides a mechanism to respond to altered redox conditions. Our data suggest that MpTCP1 activity could thereby have contributed to diversification of land plant morphologies and to adaptations to abiotic and biotic challenges, experienced by liverworts during early land plant colonization
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LSD1-mediated enhancer silencing attenuates retinoic acid signalling during pancreatic endocrine cell development.
Developmental progression depends on temporally defined changes in gene expression mediated by transient exposure of lineage intermediates to signals in the progenitor niche. To determine whether cell-intrinsic epigenetic mechanisms contribute to signal-induced transcriptional responses, here we manipulate the signalling environment and activity of the histone demethylase LSD1 during differentiation of hESC-gut tube intermediates into pancreatic endocrine cells. We identify a transient requirement for LSD1 in endocrine cell differentiation spanning a short time-window early in pancreas development, a phenotype we reproduced in mice. Examination of enhancer and transcriptome landscapes revealed that LSD1 silences transiently active retinoic acid (RA)-induced enhancers and their target genes. Furthermore, prolonged RA exposure phenocopies LSD1 inhibition, suggesting that LSD1 regulates endocrine cell differentiation by limiting the duration of RA signalling. Our findings identify LSD1-mediated enhancer silencing as a cell-intrinsic epigenetic feedback mechanism by which the duration of the transcriptional response to a developmental signal is limited
An overview of the planned CCAT software system
CCAT will be a 25m diameter sub-millimeter telescope capable of operating in
the 0.2 to 2.1mm wavelength range. It will be located at an altitude of 5600m
on Cerro Chajnantor in northern Chile near the ALMA site. The anticipated first
generation instruments include large format (60,000 pixel) kinetic inductance
detector (KID) cameras, a large format heterodyne array and a direct detection
multi-object spectrometer. The paper describes the architecture of the CCAT
software and the development strategy.Comment: 17 pages, 6 figures, to appear in Software and Cyberinfrastructure
for Astronomy III, Chiozzi & Radziwill (eds), Proc. SPIE 9152, paper ID
9152-10
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