2 research outputs found
Investigation of porcupine bezoar extract combined with electroporation on HeLa cell
Current chemo-preventive agents will causes a
long term side effects in cancer sufferer. Therefore, this
research focusses on the benefits of combine both technique of
Electroporation (EP) method and with natural animal extract
in process to inhibit the proliferation of cancer cell, as a
solution to reduce or adverse effects of orthodox drugs. The
present study indicates on anti-cancer potentially of Porcupine
Bezoar Extract (PBE), also known as Hystrix Brachyuran
against HeLa cell. Cell viability of HeLa cell were determined
after HeLa cell treated with 500 V/cm and pulse duration of
100ยตs before the concentration of 80.0 ยตg/ml of Porcupine
Bezoar Extract (PBE) was added into the cell. The cell viability
and cell growth were monitored up to 48 hours with
comparative to untreated cell as a control group.This study
result proved that by combine both technique; Electroporation
(EP) and with natural animal extract Porcupine Bezoar
Extract (PBE) might open the door and it has an ability in
supressing the growth of HeLa cell
Optimization of low amplitude pulse electric field exposure on colon cancer cell for wound healing application
Pulse electric field (PEF) is a method of creating transient pores in the cell
membrane by exposing it to a high voltage electric field of short duration. Beside
having the effect of opening pores in the membrane, PEF can change the cytoskeletal
restructuring of the cell, which has an impact on the cell morphological properties
and signalling pathways. There was evidence that extracellular matrix (ECM) protein
and transforming growth factor beta (TGF-ฮฒ) can influence cellular behaviours.
Similarly, PEF was also found to modulate the cellular behaviours. However, a
combined influence of PEF and ECM on cellular behaviour which could further
enhances the cellular processes for wound healing and tissue engineering application
has not yet been investigated. Therefore, the aim of this study is to examine the effect
of PEF in combination with ECM or TGF-ฮฒs on cellular behaviour such as
attachment proliferation, adhesion, migration and alignment of HT29 cell line
monolayer in vitro. Cell-surface adhesion was studied via trypsinization assay in
which the time taken for cells to detach from the monolayer would give the means of
assessing the strength of adhesion. Cell migration was studied by creating a wound
model on a confluence monolayer of the cell in vitro. Cell alignment was
investigated via micro contact printing techniques. The PEF was found to increase
the attachment, proliferation and migration rate of the HT29 cell by 67.6, 27.1 and
108% respectively and decrease the adhesion strength of the cells by 29.2%. The
results also show that 600V/cm and 5ms electric parameter could potentially reduce
the unpleasant sensation associated with the standard electric field parameter
currently in used in electrochemotherapy. The results of the cell alignment to the
ECM proteins pattern have shown that PEF has improved the HT29 cell alignment
by more than 40%. Similarly, the PEF was found to increase the proliferation and
decrease the adhesion rate of cells in a TGF-ฮฒ3 supplement by 50.2 and 24.1%
respectively. Thus, the outcomes of this research revealed that the use of PEF in the
presence of ECM protein have potential implication in wound healing application (by
increasing the rate of cell migration and decreasing cell adhesion)