Bone substitute effect on vascularization and bone remodeling after application of phVEGF165 transfected BMSC

VEGF (vascular endothelial growth factor) promotes vascularization and remodeling of bone substitutes. The aim of this study was to examine the effect of distinct resorbable ceramic carriers on bone forming capacities of VEGF transfected bone marrow stromal cells (BMSC). A critical size defect of the radius in rabbits was filled either by a low surface scaffold called beta-TCP (tricalciumphsphate) or the high surface scaffold CDHA (calcium deficient hydroxy-apatite) loaded with autologous BMSC, which were either transfected with a control plasmid or a plasmid coding for phVEGF165. They were compared to unloaded scaffolds. Thus, six treatment groups (n = 6 in each group) were followed by X-ray over 16 weeks. After probe retrieval, the volume of new bone was measured by micro-CT scans and vascularization was assessed in histology. While only minor bone formation was found in both carriers when implanted alone, BMSC led to increased osteogenesis in both carriers. VEGF promoted vascularization of the scaffolds significantly in contrast to BMSC alone. Bone formation was increased in the beta-TCP group, whereas it was inhibited in the CDHA group that showed faster scaffold degradation. The results indicate that the interaction of VEGF transfected BMSC with resorbable ceramic carrier influences the ability to promote bone healing

Modelling the impact of treatment uncertainties in radiotherapy

Uncertainties are inevitably part of the radiotherapy process. Uncertainty in the dose deposited in the tumour exists due to organ motion, patient positioning errors, fluctuations in machine output, delineation of regions of interest, the modality of imaging used, and treatment planning algorithm assumptions among others; there is uncertainty in the dose required to eradicate a tumour due to interpatient variations in patient-specific variables such as their sensitivity to radiation; and there is uncertainty in the dose-volume restraints that limit dose to normal tissue. This thesis involves three major streams of research including investigation of the actual dose delivered to target and normal tissue, the effect of dose uncertainty on radiobiological indices, and techniques to display the dose uncertainty in a treatment planning system. All of the analyses are performed with the dose distribution from a four-field box treatment using 6 MV photons. The treatment fields include uniform margins between the clinical target volume and planning target volume of 0.5 cm, 1.0 cm, and 1.5 cm. The major work is preceded by a thorough literature review on the size of setup and organ motion errors for various organs and setup techniques used in radiotherapy. A Monte Carlo (MC) code was written to simulate both the treatment planning and delivery phases of the radiotherapy treatment. Using MC, the mean and the variation in treatment dose are calculated for both an individual patient and across a population of patients. In particular, the possible discrepancy in tumour position located from a single CT scan and the magnitude of reduction in dose variation following multiple CT scans is investigated. A novel convolution kernel to include multiple pretreatment CT scans in the calculation of mean treatment dose is derived. Variations in dose deposited to prostate and rectal wall are assessed for each of the margins and for various magnitudes of systematic and random error, and penumbra gradients. The linear quadratic model is used to calculate prostate Tumour Control Probability (TCP) incorporating an actual (modelled) delivered prostate dose. The Kallman s-model is used to calculate the normal tissue complication probability (NTCP), incorporating actual (modelled) fraction dose in the deforming rectal wall. The impact of each treatment uncertainty on the variation in the radiobiological index is calculated for the margin sizes.Thesis (Ph.D.)--Department of Physics and Mathematical Physics, 2002

Symmetry Group and Group Representations Associated to the Thermodynamic Covariance Principle (TCP)

We describe the Lie group and the group representations associated to the nonlinear Thermodynamic Coordinate Transformations (TCT). The TCT guarantee the validity of the Thermodynamic Covariance Principle (TCP) : {\it The nonlinear closure equations, i.e. the flux-force relations, everywhere and in particular outside the Onsager region, must be covariant under TCT}. In other terms, the fundamental laws of thermodynamics should be manifestly covariant under transformations between the admissible thermodynamic forces, i.e. under TCT. The TCP ensures the validity of the fundamental theorems for systems far from equilibrium. The symmetry properties of a physical system are intimately related to the conservation laws characterizing that system. Noether's theorem gives a precise description of this relation. We derive the conserved (thermodynamic) currents and, as an example of calculation, a simple system out of equilibrium where the validity of TCP is imposed at the level of the kinetic equations is also analyzed.Comment: 35 pages, 6 figure

A vehicle-to-infrastructure communication based algorithm for urban traffic control

We present in this paper a new algorithm for urban traffic light control with mixed traffic (communicating and non communicating vehicles) and mixed infrastructure (equipped and unequipped junctions). We call equipped junction here a junction with a traffic light signal (TLS) controlled by a road side unit (RSU). On such a junction, the RSU manifests its connectedness to equipped vehicles by broadcasting its communication address and geographical coordinates. The RSU builds a map of connected vehicles approaching and leaving the junction. The algorithm allows the RSU to select a traffic phase, based on the built map. The selected traffic phase is applied by the TLS; and both equipped and unequipped vehicles must respect it. The traffic management is in feedback on the traffic demand of communicating vehicles. We simulated the vehicular traffic as well as the communications. The two simulations are combined in a closed loop with visualization and monitoring interfaces. Several indicators on vehicular traffic (mean travel time, ended vehicles) and IEEE 802.11p communication performances (end-to-end delay, throughput) are derived and illustrated in three dimension maps. We then extended the traffic control to a urban road network where we also varied the number of equipped junctions. Other indicators are shown for road traffic performances in the road network case, where high gains are experienced in the simulation results.Comment: 6 page

FAST TCP: Motivation, Architecture, Algorithms, Performance

We describe FAST TCP, a new TCP congestion control algorithm for high-speed long-latency networks, from design to implementation. We highlight the approach taken by FAST TCP to address the four difficulties which the current TCP implementation has at large windows. We describe the architecture and summarize some of the algorithms implemented in our prototype. We characterize its equilibrium and stability properties. We evaluate it experimentally in terms of throughput, fairness, stability, and responsiveness

Cooling neutron stars and superfluidity in their interiors

We study the heat capacity and neutrino emission reactions (direct and modified Urca processes, nucleon-nucleon bremsstrahlung, Cooper pairing of nucleons) in matter of supranuclear density of the neutron star cores with superfluid neutrons and protons. Various superfluidity types are analysed (singlet-state pairing and two types of triplet-state pairing, without and with nodes of the gap at a nucleon Fermi surface). The results are used for cooling simulations of isolated neutron stars. Both, the standard cooling and the cooling enhanced by the direct Urca process, are strongly affected by nucleon superfluidity. Comparison of cooling theory of isolated neutron stars with observations of their thermal radiation may give stringent constraints on the critical temperatures of the neutron and proton superfluidities in the neutron star cores.Comment: LaTeX, 85 pages, 23 figures, Physics - Uspekhi (accepted

Microfield Fluctuations and Spectral Line Shapes in Strongly Coupled Two-Component Plasmas

The spectral line shapes for hydrogen-like heavy ion emitters embedded in strongly correlated two-component electron-ion plasmas are investigated with numerical simulations. For that purpose the microfield fluctuations are calculated by molecular dynamics simulations where short range quantum effects are taken into account by using a regularized Coulomb potential for the electron-ion interaction. The microfield fluctuations are used as input in a numerical solution of the time-dependent Schroedinger equation for the radiating electron. In distinction to the standard impact and quasistatic approximations the method presented here allows to account for the correlations between plasma ions and electrons. The shapes of the Ly-alpha line in Al are investigated in the intermediate regime. The calculations are in good agreement with experiments on the Ly-alpha line in laser generated plasmas.Comment: 5 figure