Effect of Endophytic Serratia marcescens Isolated from Bryophyllum pinnatum against Clinical Bacterial Isolates

The world's health is being threatened by antimicrobial resistance (AMR). According to the World Health Organization, it is one of the top ten worldwide public health problems facing humanity. Serratia marcescens is an opportunistic pathogen that can be isolated from soil, plants, water, and air. Additionally, Serratia species offer a valuable supply of secondary metabolites that are comparatively underutilised and may have anti-MDR pathogenic properties. The present research aimed to determine the antibacterial potential of Serratia marcescens isolated from the leaves of Bryophyllum pinnatum against clinical bacterial isolates. The leaves of Bryophyllum pinnatum were collected, surface sterilised, cultured at 37 °C for 24 hours and identified utilising viteks 2 automated techniques and molecular methods. The crude metabolites extract of Serratia marcescens were extracted and utilised for antibacterial susceptibility testing using agar healthy diffusion methods. The data were measured in the diameter zone of inhibition. This study revealed six endophytic bacteria were isolated from Bryophyllum pinnatum following standard microbiological culture methods. The endophytic bacteria isolate tag L03 was found to be Gram-Negative Rod. The isolate was tentatively identified as Serratia ficaria and molecularly identified as Serratia marcescens. The metabolites of Serratia marcescens endophytes revealed a significant antibacterial activity on Klebsiella pneumoniae with a diameter zone of inhibition of 17.7 mm at 100% concentration, followed by Staphylococcus sciuri with a 12.7 mm diameter zone of inhibition. These results suggested that endophytic bacteria Serratia marcescens were isolated from the leaves of Bryophyllum pinnatum and had shown potent antibacterial activity that could be employed to create new antibacterial agents

Handling Strategies for Areas and Slums Settlements with the Implementation of Sustainable Development Goals in Mojokerto Regency, Indonesia

The new direction in the current development process is the implementation of the Sustainable Development Goals (SDGs). The concept of sustainable development is organised into four dimensions: economic, social, environmental and institutional. The Sustainable Development Goals have 17 goals and several indicators for their measurement. The United Nations (UN) 2015 formulated these 17 points of Sustainable Development Goals (SDGs) to overcome the problems that exist in the world. One of the goals contained in the SDG's is goal No 11, namely Sustainable Cities and Communities. One of the world problems discussed in this goal is slums found in Target 11.1 points, namely: "Ensure access to decent, safe and affordable housing and basic services for all and improve the quality of slums". This research method uses mixed methods and then solves with qualitative because it can produce a study of existing phenomena. In this case, case studies greatly help handle settlements and slums in other places and different handling methods as a comparative study. Mojokerto's success in addressing slums in Kedungmaling Village was achieved through strategic integration of various aspects: involving the community as active actors and working group formers, intensive on-the-ground mentoring with facilitators, land management without eviction while consolidating public spaces, strong commitment from local institutions, and program integration through DAK Integration and funding collaboration. This approach, which is aligned with the Sustainable Development Goals (SDG's), not only targets local solutions but also steers Mojokerto towards international standards of slum upgrading, making a significant impact at both local and national levels

Discovering Jewish Studies Collections in Academic Libraries: A Practical Guide

The U.S. colleges and universities offering non-sectarian educational programs in Jewish Studies rely on the support of their academic libraries for research materials and library services. For college libraries which use Library of Congress Classification scheme, it is a common practice to integrate studies resources into their general library collections. Since Jewish Studies sources span a vast number of subjects within all major disciplines, shelving integration leads to the dispersion of all relevant sources and such dispersion in turn leads to a variety of problems for library professionals and library users. For collection development librarians the problems range from lack of information about collection\u27s size, strengths or weaknesses, and for library users interested in browsing the collection, dispersion of subjects creates a major roadblock. This practical guide aims at providing a solution to such problems. By identifying all relevant Library of Congress call numbers and the corresponding Library of Congress subject headings, the guide offers a simplified access to Jewish Studies sources in general library collections. It is arranged by four major discipline: Arts & Humanities, Social Sciences, Sciences, and General Works & Bibliographies. Within each discipline, specific LC call number ranges and corresponding subjects are listed. The subjects are further subdivided and precisely identified. The guide will assist collection development librarians, library liaisons, grants and fundraising professionals and especially the Jewish Studies faculty and students, in identifying and locating relevant sources