4,808 research outputs found
Computational structured illumination for high-content fluorescent and phase microscopy
High-content biological microscopy targets high-resolution imaging across
large fields-of-view (FOVs). Recent works have demonstrated that computational
imaging can provide efficient solutions for high-content microscopy. Here, we
use speckle structured illumination microscopy (SIM) as a robust and
cost-effective solution for high-content fluorescence microscopy with
simultaneous high-content quantitative phase (QP). This multi-modal
compatibility is essential for studies requiring cross-correlative biological
analysis. Our method uses laterally-translated Scotch tape to generate
high-resolution speckle illumination patterns across a large FOV. Custom
optimization algorithms then jointly reconstruct the sample's super-resolution
fluorescent (incoherent) and QP (coherent) distributions, while digitally
correcting for system imperfections such as unknown speckle illumination
patterns, system aberrations and pattern translations. Beyond previous linear
SIM works, we achieve resolution gains of 4x the objective's
diffraction-limited native resolution, resulting in 700 nm fluorescence and 1.2
um QP resolution, across a FOV of 2x2.7 mm^2, giving a space-bandwidth product
(SBP) of 60 megapixels
Micro Fourier Transform Profilometry (FTP): 3D shape measurement at 10,000 frames per second
Recent advances in imaging sensors and digital light projection technology
have facilitated a rapid progress in 3D optical sensing, enabling 3D surfaces
of complex-shaped objects to be captured with improved resolution and accuracy.
However, due to the large number of projection patterns required for phase
recovery and disambiguation, the maximum fame rates of current 3D shape
measurement techniques are still limited to the range of hundreds of frames per
second (fps). Here, we demonstrate a new 3D dynamic imaging technique, Micro
Fourier Transform Profilometry (FTP), which can capture 3D surfaces of
transient events at up to 10,000 fps based on our newly developed high-speed
fringe projection system. Compared with existing techniques, FTP has the
prominent advantage of recovering an accurate, unambiguous, and dense 3D point
cloud with only two projected patterns. Furthermore, the phase information is
encoded within a single high-frequency fringe image, thereby allowing
motion-artifact-free reconstruction of transient events with temporal
resolution of 50 microseconds. To show FTP's broad utility, we use it to
reconstruct 3D videos of 4 transient scenes: vibrating cantilevers, rotating
fan blades, bullet fired from a toy gun, and balloon's explosion triggered by a
flying dart, which were previously difficult or even unable to be captured with
conventional approaches.Comment: This manuscript was originally submitted on 30th January 1
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Lensfree computational microscopy tools for cell and tissue imaging at the point-of-care and in low-resource settings.
The recent revolution in digital technologies and information processing methods present important opportunities to transform the way optical imaging is performed, particularly toward improving the throughput of microscopes while at the same time reducing their relative cost and complexity. Lensfree computational microscopy is rapidly emerging toward this end, and by discarding lenses and other bulky optical components of conventional imaging systems, and relying on digital computation instead, it can achieve both reflection and transmission mode microscopy over a large field-of-view within compact, cost-effective and mechanically robust architectures. Such high throughput and miniaturized imaging devices can provide a complementary toolset for telemedicine applications and point-of-care diagnostics by facilitating complex and critical tasks such as cytometry and microscopic analysis of e.g., blood smears, Pap tests and tissue samples. In this article, the basics of these lensfree microscopy modalities will be reviewed, and their clinically relevant applications will be discussed
High-speed in vitro intensity diffraction tomography
We demonstrate a label-free, scan-free intensity diffraction tomography technique utilizing annular illumination (aIDT) to rapidly characterize large-volume three-dimensional (3-D) refractive index distributions in vitro. By optimally matching the illumination geometry to the microscope pupil, our technique reduces the data requirement by 60 times to achieve high-speed 10-Hz volume rates. Using eight intensity images, we recover volumes of ∼350 μm  ×  100 μm  ×  20  μm, with near diffraction-limited lateral resolution of   ∼  487  nm and axial resolution of   ∼  3.4  μm. The attained large volume rate and high-resolution enable 3-D quantitative phase imaging of complex living biological samples across multiple length scales. We demonstrate aIDT’s capabilities on unicellular diatom microalgae, epithelial buccal cell clusters with native bacteria, and live Caenorhabditis elegans specimens. Within these samples, we recover macroscale cellular structures, subcellular organelles, and dynamic micro-organism tissues with minimal motion artifacts. Quantifying such features has significant utility in oncology, immunology, and cellular pathophysiology, where these morphological features are evaluated for changes in the presence of disease, parasites, and new drug treatments. Finally, we simulate the aIDT system to highlight the accuracy and sensitivity of the proposed technique. aIDT shows promise as a powerful high-speed, label-free computational microscopy approach for applications where natural imaging is required to evaluate environmental effects on a sample in real time.https://arxiv.org/abs/1904.06004Accepted manuscrip
Improving spatial resolution of confocal Raman microscopy by super-resolution image restoration
A new super-resolution image restoration confocal Raman microscopy method (SRIR-RAMAN) is proposed for improving the spatial resolution of confocal Raman microscopy. This method can recover the lost high spatial frequency of the confocal Raman microscopy by using Poisson-MAP super-resolution imaging restoration, thereby improving the spatial resolution of confocal Raman microscopy and realizing its super-resolution imaging. Simulation analyses and experimental results indicate that the spatial resolution of SRIR-RAMAN can be improved by 65% to achieve 200 nm with the same confocal Raman microscopy system. This method can provide a new tool for high spatial resolution micro-probe structure detection in physical chemistry, materials science, biomedical science and other areas
Roadmap on structured light
Structured light refers to the generation and application of custom light fields. As the tools and technology to create and detect structured light have evolved, steadily the applications have begun to emerge. This roadmap touches on the key fields within structured light from the perspective of experts in those areas, providing insight into the current state and the challenges their respective fields face. Collectively the roadmap outlines the venerable nature of structured light research and the exciting prospects for the future that are yet to be realized.Peer ReviewedPostprint (published version
Far-field Super-resolution Chemical Microscopy
Far-field chemical microscopy providing molecular electronic or vibrational
fingerprint information opens a new window for the study of three-dimensional
biological, material, and chemical systems. Chemical microscopy provides a
nondestructive way of chemical identification without exterior labels. However,
the diffraction limit of optics hindered it from discovering more details under
the resolution limit. Recent development of super-resolution techniques gives
enlightenment to open this door behind far-field chemical microscopy. Here, we
review recent advances that have pushed the boundary of far-field chemical
microscopy in terms of spatial resolution. We further highlight applications in
biomedical research, material characterization, environmental study, cultural
heritage conservation, and integrated chip inspection.Comment: 34 pages, 8 figures,1 tabl
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