7 research outputs found
Predicting microRNA modulation in human prostate cancer using a simple String IDentifier (SID1.0).
To make faster and efficient the identification of mRNA targets common to more than one miRNA, and to identify new miRNAs modulated in specific pathways, a computer program identified as SID1.0 (simple String IDentifier) was developed and successfully applied in the identification of deregulated miRNAs in prostate cancer cells. This computationally inexpensive Fortran program is based on the strategy of exhaustive search and specifically designed to screen shared data (target genes, miRNAs and pathways) available from PicTar and DIANA-MicroT 3.0 databases. As far as we know this is the first software designed to filter data retrieved from available miRNA databases. SID1.0 takes advantage of the standard Fortran intrinsic functions for manipulating text strings and requires ASCII input files. In order to demonstrate SID1.0 applicability, some miRNAs expected from the literature to associate with cancerogenesis (miR-125b, miR-148a and miR-141), were randomly identified as main entries for SID1.0 to explore matching sequences of mRNA targets and also to explore KEGG pathways for the presence of ID codes of targeted genes. Besides genes and pathways already described in the literature, SID1.0 has proven to useful for predicting other genes involved in prostate carcinoma. These latter were used to identify new deregulated miRNAs: miR-141, miR-148a, miR-19a and miR-19b. Prediction data were preliminary confirmed by expression analysis of the identified miRNAs in androgen-dependent (LNCaP) and independent (PC3) prostate carcinoma cell lines and in normal prostatic epithelial cells (PrEC)
Hormone replacement therapy enhances IGF-1 signaling in skeletal muscle by diminishing miR-182 and miR-223 expressions: a study on postmenopausal monozygotic twin pairs
MiRNAs are fine-tuning modifiers of skeletal muscle regulation, but knowledge of
their hormonal control is lacking. We used a co-twin case-control study design,
that is, monozygotic postmenopausal twin pairs discordant for estrogen-based
hormone replacement therapy (HRT) to explore estrogen-dependent skeletal muscle
regulation via miRNAs. MiRNA profiles were determined from vastus lateralis
muscle of nine healthy 54-62-years-old monozygotic female twin pairs discordant
for HRT (median 7 years). MCF-7 cells, human myoblast cultures and mouse muscle
experiments were used to confirm estrogen's causal role on the expression of
specific miRNAs, their target mRNAs and proteins and finally the activation of
related signaling pathway. Of the 230 miRNAs expressed at detectable levels in
muscle samples, qPCR confirmed significantly lower miR-182, miR-223 and
miR-142-3p expressions in HRT using than in their nonusing co-twins.
Insulin/IGF-1 signaling emerged one common pathway targeted by these miRNAs.
IGF-1R and FOXO3A mRNA and protein were more abundantly expressed in muscle
samples of HRT users than nonusers. In vitro assays confirmed effective targeting
of miR-182 and miR-223 on IGF-1R and FOXO3A mRNA as well as a dose-dependent
miR-182 and miR-223 down-regulations concomitantly with up-regulation of FOXO3A
and IGF-1R expression. Novel finding is the postmenopausal HRT-reduced miRs-182,
miR-223 and miR-142-3p expression in female skeletal muscle. The observed
miRNA-mediated enhancement of the target genes' IGF-1R and FOXO3A expression as
well as the activation of insulin/IGF-1 pathway signaling via phosphorylation of
AKT and mTOR is an important mechanism for positive estrogen impact on skeletal
muscle of postmenopausal women
Predicting microRNA modulation in human prostate cancer using a simple String IDentifier (SID1.0)
To make faster and efficient the identification of mRNA targets common to more than one miRNA, and to
identify new miRNAs modulated in specific pathways, a computer program identified as SID1.0 (simple
String IDentifier) was developed and successfully applied in the identification of deregulated miRNAs in
prostate cancer cells. This computationally inexpensive Fortran program is based on the strategy of
exhaustive search and specifically designed to screen shared data (target genes, miRNAs and pathways)
available from PicTar and DIANA-MicroT 3.0 databases. As far as we know this is the first software
designed to filter data retrieved from available miRNA databases. SID1.0 takes advantage of the standard
Fortran intrinsic functions for manipulating text strings and requires ASCII input files. In order to demonstrate
SID1.0 applicability, some miRNAs expected from the literature to associate with cancerogenesis
(miR-125b, miR-148a and miR-141), were randomly identified as main entries for SID1.0 to explore
matching sequences of mRNA targets and also to explore KEGG pathways for the presence of ID codes
of targeted genes. Besides genes and pathways already described in the literature, SID1.0 has proven
to useful for predicting other genes involved in prostate carcinoma. These latter were used to identify
new deregulated miRNAs: miR-141, miR-148a, miR-19a and miR-19b. Prediction data were preliminary
confirmed by expression analysis of the identified miRNAs in androgen-dependent (LNCaP) and independent
(PC3) prostate carcinoma cell lines and in normal prostatic epithelial cells (PrEC
Predicting microRNA modulation in human prostate cancer using a simple String IDentifier (SID1.0)
none9noTo make faster and efficient the identification of mRNA targets common to more than one miRNA, and to
identify new miRNAs modulated in specific pathways, a computer program identified as SID1.0 (simple
String IDentifier) was developed and successfully applied in the identification of deregulated miRNAs in
prostate cancer cells. This computationally inexpensive Fortran program is based on the strategy of
exhaustive search and specifically designed to screen shared data (target genes, miRNAs and pathways)
available from PicTar and DIANA-MicroT 3.0 databases. As far as we know this is the first software
designed to filter data retrieved from available miRNA databases. SID1.0 takes advantage of the standard
Fortran intrinsic functions for manipulating text strings and requires ASCII input files. In order to demonstrate
SID1.0 applicability, some miRNAs expected from the literature to associate with cancerogenesis
(miR-125b, miR-148a and miR-141), were randomly identified as main entries for SID1.0 to explore
matching sequences of mRNA targets and also to explore KEGG pathways for the presence of ID codes
of targeted genes. Besides genes and pathways already described in the literature, SID1.0 has proven
to useful for predicting other genes involved in prostate carcinoma. These latter were used to identify
new deregulated miRNAs: miR-141, miR-148a, miR-19a and miR-19b. Prediction data were preliminary
confirmed by expression analysis of the identified miRNAs in androgen-dependent (LNCaP) and independent
(PC3) prostate carcinoma cell lines and in normal prostatic epithelial cells (PrEC)N/ArestrictedM.C. ALBERTINI; F. OLIVIERI; R. LAZZARINI; F. PILOLLI; F. GALLI; G. SPADA; A. ACCORSI; M.R. RIPPO; A.D. PROCOPIOAlbertini, MARIA CRISTINA; F., Olivieri; R., Lazzarini; F., Pilolli; F., Galli; Spada, Giorgio; Accorsi, Augusto; M. R., Rippo; A. D., Procopi