p21-activated kinase (PAK) regulates cytoskeletal reorganization and directional migration in human neutrophils

Neutrophils serve as a first line of defense in innate immunity owing in part to their ability to rapidly migrate towards chemotactic factors derived from invading pathogens. As a migratory function, neutrophil chemotaxis is regulated by the Rho family of small GTPases. However, the mechanisms by which Rho GTPases orchestrate cytoskeletal dynamics in migrating neutrophils remain ill-defined. In this study, we characterized the role of p21-activated kinase (PAK) downstream of Rho GTPases in cytoskeletal remodeling and chemotactic processes of human neutrophils. We found that PAK activation occurred upon stimulation of neutrophils with f-Met-Leu-Phe (fMLP), and PAK accumulated at the actin-rich leading edge of stimulated neutrophils, suggesting a role for PAK in Rac-dependent actin remodeling. Treatment with the pharmacological PAK inhibitor, PF3758309, abrogated the integrity of RhoA-mediated actomyosin contractility and surface adhesion. Moreover, inhibition of PAK activity impaired neutrophil morphological polarization and directional migration under a gradient of fMLP, and was associated with dysregulated Ca2+ signaling. These results suggest that PAK serves as an important effector of Rho-family GTPases in neutrophil cytoskeletal reorganization, and plays a key role in driving efficient directional migration of human neutrophils

Use of potassium polyaspartate for the tartaric stabilization of Sicilian white wines

Cold stabilization is a common method used to avoid tartaric acid crystals from forming in bottled wine, but this technique shows some inconveniences on the sensory characteristics and energy cost. In the present research, the tartaric stabilization in Sicilian white wines, with a recently permissible molecule in oenology, was studied: the potassium polyaspartate (PAK). The PAK has a negative charge at wine pH and allows to sequester the K+ cations; consequently it inhibits the formation and the growth of potassium bitartrate crystals. PAK is a relatively small polymer; perfectly microfiltrated and does not allow the filling phenomena in filtration membranes. The adding of PAK allowed to reduce almost all the tartaric precipitations on very unstable wines, regardless of the chemical-physical characteristics of the treated white wines. The stability of PAK, after thermal stress of the wine added to this polymer, was confirmed evaluating different analytical parameters such as pH, total acidity and buffer power, while variations in the conductivity of wines seemed to indicate a residual tartaric instability. In conclusion, the use of PAK makes possible to improve the sensory characteristics of wines, considering lower losses of tartaric acid and potassium, which are important for acid perception and acid persistence (buffer power), as well as reducing production costs and low environmental impact

Precision measurement of the D∗0D^{*0} decay branching fractions

Using 482 pb$^{-1}$ of data taken at $\sqrt{s}=4.009$ GeV, we measure the branching fractions of the decays of $D^{*0}$ into $D^0\pi^0$ and $D^0\gamma$ to be \BR(D^{*0} \to D^0\pi^0)=(65.5\pm 0.8\pm 0.5)% and \BR(D^{*0} \to D^0\gamma)=(34.5\pm 0.8\pm 0.5)% respectively, by assuming that the $D^{*0}$ decays only into these two modes. The ratio of the two branching fractions is \BR(D^{*0} \to D^0\pi^0)/\BR(D^{*0} \to D^0\gamma) =1.90\pm 0.07\pm 0.05, which is independent of the assumption made above. The first uncertainties are statistical and the second ones systematic. The precision is improved by a factor of three compared to the present world average values

History of Catalan numbers

We give a brief history of Catalan numbers, from their first discovery in the 18th century to modern times. This note will appear as an appendix in Richard Stanley's forthcoming book on Catalan numbers.Comment: 10 page

The area of cyclic polygons: Recent progress on Robbins' Conjectures

In his works [R1,R2] David Robbins proposed several interrelated conjectures on the area of the polygons inscribed in a circle as an algebraic function of its sides. Most recently, these conjectures have been established in the course of several independent investigations. In this note we give an informal outline of these developments.Comment: To appear in Advances Applied Math. (special issue in memory of David Robbins