Dual-Mode Electro-Optical Techniques for Biosensing Applications : A Review

The monitoring of biomolecular interactions is a key requirement for the study of complex biological processes and the diagnosis of disease. Technologies that are capable of providing label-free, real-time insight into these interactions are of great value for the scientific and clinical communities. Greater understanding of biomolecular interactions alongside increased detection accuracy can be achieved using technology that can provide parallel information about multiple parameters of a single biomolecular process. For example, electro-optical techniques combine optical and electrochemical information to provide more accurate and detailed measurements that provide unique insights into molecular structure and function. Here, we present a comparison of the main methods for electro-optical biosensing, namely, electrochemical surface plasmon resonance (EC-SPR), electrochemical optical waveguide lightmode spectroscopy (EC-OWLS), and the recently reported silicon-based electrophotonic approach. The comparison considers different application spaces, such as the detection of low concentrations of biomolecules, integration, the tailoring of light-matter interaction for the understanding of biomolecular processes, and 2D imaging of biointeractions on a surface

From microfluidics to nanofluidics and signal wave-guiding for nanophotonics, biophotonics resolution and drug delivery applications

The aim of this Review was to show based on recent microfluidics developments how it could be incorporated within new Advanced Clinical Research for Future Nanomedicine treatments the design of versatile Micro and Nanodevices. These developments were done by a control of the Nanoscale for applications from Nanophotonics to Genomics, Biophotonics and Drug Delivery studies accompanied with enhanced Imaging resolutions and faster diagnosis and treatments. In addition it was discussed the last Noble Prize in Physics 2019 related with Laser phenomena and development of instrumentation for Medical applications as individual Biostructure trapping and eye chirurgical interventions. By this manner the main goal of this short Review was to give an opinion about the state of the Art of Microfluidics to Nanofluidics with incorporation of Lasers in different optical approaches, applications and Future perspectives.Fil: Salinas, Carina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; ArgentinaFil: Bracamonte, Angel Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Físico-química de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Instituto de Investigaciones en Físico-química de Córdoba; Argentin

Review of Electrochemically Triggered Macromolecular Film Buildup Processes and Their Biomedical Applications

International audienceMacromolecular coatings play an important role in many technological areas, ranging from the car industry to biosensors. Among the different coating technologies, electrochemically triggered processes are extremely powerful because they allow in particular spatial confinement of the film buildup up to the micrometer scale on microelectrodes. Here, we review the latest advances in the field of electrochemically triggered macromolecular film buildup processes performed in aqueous solutions. All these processes will be discussed and related to their several applications such as corrosion prevention, biosensors, antimicrobial coatings, drug-release, barrier properties and cell encapsulation. Special emphasis will be put on applications in the rapidly growing field of biosensors. Using polymers or proteins, the electrochemical buildup of the films can result from a local change of macromolecules solubility, self-assembly of polyelectrolytes through electrostatic/ionic interactions or covalent cross-linking between different macromolecules. The assembly process can be in one step or performed step-by-step based on an electrical trigger affecting directly the interacting macromolecules or generating ionic species

Perspectivas y estrategias de Biodetección y nuevos tratamientos en Nanomedicina con un enfoque sobre el Corona Virus

El diseño de nuevas drogas, moléculas, Nano- y Microestructuras paradeterminadas aplicaciones durante el paso del tiempo en la historia de la química y análisis clínico mostro ser de gran impacto en ciencias de la vida. En este contexto, y principalmente ante la Pandemia del Corona Virus, se debe resaltar la importancia de una respuesta rápida en lo que respecta a nuevas estrategias de detección y desarrollo de nuevos tratamientos. Así, el diseño de nuevos sistemas de detecciónmas sensibles acompañados con tratamientos más eficaces son requeridos. En este sentido, en esta comunicación se discutieron las últimas publicaciones más reciente de más alto impacto relacionadas con la detección del Corona Virus y tratamiento al SARS CoV2. De esta manera se discutieron los siguientes tópicos: i) la importancia de las interacciones no- covalentes en los eventos de reconocimiento de anticuerpo-antígeno, ii)la importancia del el factor genómico, iii) la utilización de interacciones no-covalentes en diferentes sistemas de detección, Nanoplataformas de diagnóstico, y otros arreglos en la Nano-, y Micro-escala al igual que nuevos dispositivos s instrumentales. Además, se discutió tópico relacionado del desarrollo de nuevos tratamientos en progreso, vacunas, enla presente situación de pandemia. Entonces, en base a la presentación de los anteriores temas se intentó estimular el desarrollo mejoramiento de nuevos dispositivos Multifuncionales Fotónicos acoplados a nuevos tratamientos en tiempo real al momento de la misma detección.publishedVersionFil: Amé Martin. Hospital Privado e Instituto Universitario de Ciencias Biomédicas de Córdoba, Córdoba; Argentina.Fil: Abu Serea, EsraaSamy. BCMaterials, Basque Center for Materials. Applications and Nanostructures, Martina Casiano; España.Fil: Abu Serea, EsraaSamy. Central Metallurgical Research and Development Institute, Cairo; Egipto.Fil: EsmailShalan, Ahmed. BCMaterials, Basque Center for Materials. Applications and Nanostructures, Martina Casiano; España.Fil: EsmailShalan, Ahmed. Central Metallurgical Research and Development Institute, Cairo; Egipto.Fil: Bracamonte, A. Guillermo. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina.Fil: Bracamonte, A. Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Físico Química de Córdoba; Argentina.Fil: Bracamonte, A. Guillermo. Université Laval. Departement de chimie and Centre d’optique, photonique et laser; Québec; Canadá

Chromatographic Techniques for Estimation of Aflatoxins in Food Commodities

Aflatoxins, produced mainly by Aspergillus flavus Aspergillus parasiticus, have been documented as one of the major food contaminants throughout the world. Because of their toxic nature, these food contaminants have acknowledged considerable attention in recent years. Among the different types of Aflatoxins, the most prevalent and predominant Aflatoxins are AFB1, AFB2, AFG1, AFG2, AFM1, AFM2 which are considered the more lethal as compared to others. Several analytical and immunological methods are available for testing and estimating aflatoxins in different food commodities. However, chromatographic techniques have been considered superior regarding the estimation of aflatoxins both qualitatively and quantitatively. Chromatographic techniques have numerous applications for the separation and identification of chemical and biological compounds in food industry. It has grown to be the most popular and versatile of all analytical techniques in laboratories used for the analysis of multiple components in different matrices. For preliminary qualitative detection of Aflatoxins, Thin layer chromatography (TLC) is considered the best analytical technique which is being used broadly in food industry. However, liquid chromatographic techniques including High Performance Liquid Chromatography (HPLC) and Liquid chromatography-mass Spectrometry (LC–MS) are the best analytical techniques developed so far for the quantification of Aflatoxins in food commodities