BeppoSAX Detection and Follow-up of GRB980425

We present BeppoSAX GRBM and WFC light curves of GRB980425 and NFI follow-up data taken in 1998 April, May, and November. The first NFI observation has detected within the 8' radius error box of the GRB an X-ray source positionally consistent with the supernova SN 1998bw, exploded within a day of GRB980425, and a fainter X-ray source, not consistent with the position of the supernova. The former source is detected in the following NFI pointings and exhibits a decline of a factor of two in six months. If it is associated with SN 1998bw, this is the first detection of hard X-ray emission from a Type I supernova. The latter source exhibits only marginally significant variability. Based on these data, it is not possible to select either source as a firm candidate for the GRB counterpart.Comment: 2 pages, 1 PostScript figure, submitted to A&AS, Proc. of the Conference "Gamma-Ray Bursts in the Afterglow Era", held in Rome, 1998 November 3-6. Results concerning 'Source 2' have been update

nfi-1 affects behavior and life-span in C. elegans but is not essential for DNA replication or survival

BACKGROUND: The Nuclear Factor I (one) (NFI) family of transcription/replication factors plays essential roles in mammalian gene expression and development and in adenovirus DNA replication. Because of its role in viral DNA replication NFI has long been suspected to function in host DNA synthesis. Determining the requirement for NFI proteins in mammalian DNA replication is complicated by the presence of 4 NFI genes in mice and humans. Loss of individual NFI genes in mice cause defects in brain, lung and tooth development, but the presence of 4 homologous NFI genes raises the issue of redundant roles for NFI genes in DNA replication. No NFI genes are present in bacteria, fungi or plants. However single NFI genes are present in several simple animals including Drosophila and C. elegans, making it possible to test for a requirement for NFI in multicellular eukaryotic DNA replication and development. Here we assess the functions of the single nfi-1 gene in C. elegans. RESULTS: C. elegans NFI protein (CeNFI) binds specifically to the same NFI-binding site recognized by vertebrate NFIs. nfi-1 encodes alternatively-spliced, maternally-inherited transcripts that are expressed at the single cell stage, during embryogenesis, and in adult muscles, neurons and gut cells. Worms lacking nfi-1 survive but have defects in movement, pharyngeal pumping and egg-laying and have a reduced life-span. Expression of the muscle gene Ce titin is decreased in nfi-1 mutant worms. CONCLUSION: NFI gene function is not needed for survival in C. elegans and thus NFI is likely not essential for DNA replication in multi-cellular eukaryotes. The multiple defects in motility, egg-laying, pharyngeal pumping, and reduced lifespan indicate that NFI is important for these processes. Reduction in Ce titin expression could affect muscle function in multiple tissues. The phenotype of nfi-1 null worms indicates that NFI functions in multiple developmental and behavioral systems in C. elegans, likely regulating genes that function in motility, egg-laying, pharyngeal pumping and lifespan maintenance

Hierarchical spatial models for predicting tree species assemblages across large domains

Spatially explicit data layers of tree species assemblages, referred to as forest types or forest type groups, are a key component in large-scale assessments of forest sustainability, biodiversity, timber biomass, carbon sinks and forest health monitoring. This paper explores the utility of coupling georeferenced national forest inventory (NFI) data with readily available and spatially complete environmental predictor variables through spatially-varying multinomial logistic regression models to predict forest type groups across large forested landscapes. These models exploit underlying spatial associations within the NFI plot array and the spatially-varying impact of predictor variables to improve the accuracy of forest type group predictions. The richness of these models incurs onerous computational burdens and we discuss dimension reducing spatial processes that retain the richness in modeling. We illustrate using NFI data from Michigan, USA, where we provide a comprehensive analysis of this large study area and demonstrate improved prediction with associated measures of uncertainty.Comment: Published in at http://dx.doi.org/10.1214/09-AOAS250 the Annals of Applied Statistics (http://www.imstat.org/aoas/) by the Institute of Mathematical Statistics (http://www.imstat.org

BeppoSAX Observations of GRB980425: Detection of the Prompt Event and Monitoring of the Error Box

We present BeppoSAX follow-up observations of GRB980425 obtained with the Narrow Field Instruments (NFI) in April, May, and November 1998. The first NFI observation has detected within the 8' radius error box of the GRB an X-ray source positionally consistent with the supernova 1998bw, which exploded within a day of GRB980425, and a fainter X-ray source, not consistent with the position of the supernova. The former source is detected in the following NFI pointings and exhibits a decline of a factor of two in six months. If it is associated with SN 1998bw, this is the first detection of X-ray emission from a Type I supernova above 2 keV. The latter source exhibits only marginally significant variability. The X-ray spectra and variability of the supernova are compared with thermal and non-thermal models of supernova high energy emission. Based on the BeppoSAX data, it is not possible to firmly establish which of the two detected sources is the GRB X-ray counterpart, although probability considerations favor the supernova.Comment: 16 pages, Latex, 6 PostScript figures and 1 GIF figure, 2 tables, submitted to The Astrophysical Journa

Creating a Father-Friendly Library: An Examination of the Importance of Fatherhood Support in Today\u27s Library Service Model

Research shows the importance of fathers being present in the lives of children.The absence of support for fathers, perpetuates a long standing generational decline in the viability of men as a whole. This work seeks to introduce librarians to the possibilities of libraries offering support for dads. With library use among males being very low, this is also an opportunity for libraries to serve those who are often forgotten. This paper illustrates how the National Fatherhood Initiative (NFI) offers a path to help dads and explains how the NFI and libraries can partner through the creation of programs

Statistical Modelling of Pre-Impact Velocities in Car Crashes

The law wants to determine if any party involved in a car crash is guilty. The Dutch court invokes the expertise of the Netherlands Forensic Institute (NFI) to answer this question. We discuss the present method of the NFI to deter- mine probabilities on pre-impact car velocities, given the evidence from the crash scene. A disadvantage of this method is that it requires a prior distribution on the velocities of the cars involved in the crash. We suggest a different approach, that of statistical significance testing, which can be carried out without a prior. We explain this method, and apply it to a toy model. Finally, a sensitivity analysis is performed on a simple two-car collision model

Functional role of Nuclear Fators-I in hematopoietic ontogenesis

Nuclear Factor I (NFI) transcriptional factors constitute a family of four members, NFI-A, B, C and X, known for their positive and negative transcriptional regulatory roles in a cell type and promoter specific context. We previously identified NFI-A as a relevant target of the myeloid regulator microRNA-223, then we found that its levels play a key role in directing hematopoietic progenitors to the erythroid or granulocytic lineage. This prompted us to examine whether the expression of NFI-A and/or other NFIs factors could regulate primitive and definitive hematopoiesis in vivo. To this end we initially studied the expression pattern of NFIs factors in different tissues and stages of embryo development of CD1 mice. Our preliminary results indicate that NFI-A presents the most interesting expression pattern among NFIs factors, being express in hematopoietic tissues earlier and at the highest level during embryo development. In addition, performing colony-forming progenitor assays, we found NFI-A expression in primitive erythroid progenitor and during definitive hematopoietic colonies production, implicating it in having a possible role in primitive and definitive hematopoiesis. To elucidate the role of NFI-A in hematopoiesis we used two different strains of NFI-A-/- mice: B6N31 and B6hyb129mice. Histological examinations of hematopoietic tissues of NFI-A-/- mice showed that Nfi-A disruption results in hypocellularity of hematopoietic compartment together with a marked decrease of M/E ratio. Genes expression analysis performed on B6N31 hematopoietic tissues indicates that NFI-A -/- mice have a delay in the repression of embryonic β-globins and a perinatal decrease in adult globins expression, suggesting an involvement for NFI-A in the control of β-globins switching. In addiction NFI-A -/- hematopoietic tissues presents an up-regulation of NFI-B expression, indicating its possible action as compensator of NFI-A. To investigate about the role of NFI-A in adult hematopoiesis, we performed complete blood counts of peripheral blood from adults B6N31 NFI-A +/- and we observed a decreased MCV, an increased RDW and a decreased MCH compared with adult NFI-A +/+ B6N31 mice, demonstrating an haploinsufficiency of NFI-A factor and an altered hemoglobin synthesis. These data indicates that NFI-A could be involved in the pathogenesis of hematological diseases, further underlying its importance in hematopoietic development

Temporal regulation of nuclear factor one occupancy by calcineurin/NFAT governs a voltage-sensitive developmental switch in late maturing neurons

Dendrite and synapse development are critical for establishing appropriate neuronal circuits, and disrupted timing of these events can alter neural connectivity. Using microarrays, we have identified a nuclear factor I (NFI)-regulated temporal switch program linked to dendrite formation in developing mouse cerebellar granule neurons (CGNs). NFI function was required for upregulation of many synapse-related genes as well as downregulation of genes expressed in immature CGNs. Chromatin immunoprecipitation analysis revealed that a central feature of this program was temporally regulated NFI occupancy of late-expressed gene promoters. Developing CGNs undergo a hyperpolarizing shift in membrane potential, and depolarization inhibits their dendritic and synaptic maturation via activation of calcineurin (CaN) (Okazawa et al., 2009). Maintaining immature CGNs in a depolarized state blocked NFI temporal occupancy of late-expressed genes and the NFI switch program via activation of the CaN/nuclear factor of activated T-cells, cytoplasmic (NFATc) pathway and promotion of late-gene occupancy by NFATc4, and these mechanisms inhibited dendritogenesis. Conversely, inhibition of the CaN/NFATc pathway in CGNs maturing under physiological nondepolarizing conditions upregulated the NFI switch program, NFI temporal occupancy, and dendrite formation. NFATc4 occupied the promoters of late-expressed NFI program genes in immature mouse cerebellum, and its binding was temporally downregulated with development. Further, NFI temporal binding and switch gene expression were upregulated in the developing cerebellum of Nfatc4 (-/-) mice. These findings define a novel NFI switch and temporal occupancy program that forms a critical link between membrane potential/CaN and dendritic maturation in CGNs. CaN inhibits the program and NFI occupancy in immature CGNs by promoting NFATc4 binding to late-expressed genes. As maturing CGNs become more hyperpolarized, NFATc4 binding declines leading to onset of NFI temporal binding and the NFI switch program

Crosstalk between Nuclear Factor I-C and Transforming Growth Factor-β1 Signaling Regulates Odontoblast Differentiation and Homeostasis

Transforming growth factor-β1 (TGF-β1) signaling plays a key role in vertebrate development, homeostasis, and disease. Nuclear factor I-C (NFI-C) has been implicated in TGF-β1 signaling, extracellular matrix gene transcription, and tooth root development. However, the functional relationship between NFI-C and TGF-β1 signaling remains uncharacterized. The purpose of this study was to identify the molecular interactions between NFI-C and TGF-β1 signaling in mouse odontoblasts. Real-time polymerase chain reaction and western analysis demonstrated that NFI-C expression levels were inversely proportional to levels of TGF-β1 signaling molecules during in vitro odontoblast differentiation. Western blot and immunofluorescence results showed that NFI-C was significantly degraded after TGF-β1 addition in odontoblasts, and the formation of the Smad3 complex was essential for NFI-C degradation. Additionally, ubiquitination assay results showed that Smurf1 and Smurf2 induced NFI-C degradation and polyubiquitination in a TGF-β1-dependent manner. Both kinase and in vitro binding assays revealed that the interaction between NFI-C and Smurf1/Smurf2 requires the activation of the mitogen-activated protein kinase pathway by TGF-β1. Moreover, degradation of NFI-C induced by TGF-β1 occurred generally in cell types other than odontoblasts in normal human breast epithelial cells. In contrast, NFI-C induced dephosphorylation of p-Smad2/3. These results show that crosstalk between NFI-C and TGF-β1 signaling regulates cell differentiation and homeostatic processes in odontoblasts, which might constitute a common cellular mechanism