Particle Detection Algorithms for Complex Plasmas

In complex plasmas, the behavior of freely floating micrometer sized particles is studied. The particles can be directly visualized and recorded by digital video cameras. To analyze the dynamics of single particles, reliable algorithms are required to accurately determine their positions to sub-pixel accuracy from the recorded images. Typically, straightforward algorithms are used for this task. Here, we combine the algorithms with common techniques for image processing. We study several algorithms and pre- and post-processing methods, and we investigate the impact of the choice of threshold parameters, including an automatic threshold detection. The results quantitatively show that each algorithm and method has its own advantage, often depending on the problem at hand. This knowledge is applicable not only to complex plasmas, but useful for any kind of comparable image-based particle tracking, e.g. in the field of colloids or granular matter

Inferring Latent States and Refining Force Estimates via Hierarchical Dirichlet Process Modeling in Single Particle Tracking Experiments

Optical microscopy provides rich spatio-temporal information characterizing in vivo molecular motion. However, effective forces and other parameters used to summarize molecular motion change over time in live cells due to latent state changes, e.g., changes induced by dynamic micro-environments, photobleaching, and other heterogeneity inherent in biological processes. This study focuses on techniques for analyzing Single Particle Tracking (SPT) data experiencing abrupt state changes. We demonstrate the approach on GFP tagged chromatids experiencing metaphase in yeast cells and probe the effective forces resulting from dynamic interactions that reflect the sum of a number of physical phenomena. State changes are induced by factors such as microtubule dynamics exerting force through the centromere, thermal polymer fluctuations, etc. Simulations are used to demonstrate the relevance of the approach in more general SPT data analyses. Refined force estimates are obtained by adopting and modifying a nonparametric Bayesian modeling technique, the Hierarchical Dirichlet Process Switching Linear Dynamical System (HDP-SLDS), for SPT applications. The HDP-SLDS method shows promise in systematically identifying dynamical regime changes induced by unobserved state changes when the number of underlying states is unknown in advance (a common problem in SPT applications). We expand on the relevance of the HDP-SLDS approach, review the relevant background of Hierarchical Dirichlet Processes, show how to map discrete time HDP-SLDS models to classic SPT models, and discuss limitations of the approach. In addition, we demonstrate new computational techniques for tuning hyperparameters and for checking the statistical consistency of model assumptions directly against individual experimental trajectories; the techniques circumvent the need for "ground-truth" and subjective information.Comment: 25 pages, 6 figures. Differs only typographically from PLoS One publication available freely as an open-access article at http://journals.plos.org/plosone/article?id=10.1371/journal.pone.013763

Incremental low rank noise reduction for robust infrared tracking of body temperature during medical imaging

Thermal imagery for monitoring of body temperature provides a powerful tool to decrease health risks (e.g., burning) for patients during medical imaging (e.g., magnetic resonance imaging). The presented approach discusses an experiment to simulate radiology conditions with infrared imaging along with an automatic thermal monitoring/tracking system. The thermal tracking system uses an incremental low-rank noise reduction applying incremental singular value decomposition (SVD) and applies color based clustering for initialization of the region of interest (ROI) boundary. Then a particle filter tracks the ROI(s) from the entire thermal stream (video sequence). The thermal database contains 15 subjects in two positions (i.e., sitting, and lying) in front of thermal camera. This dataset is created to verify the robustness of our method with respect to motion-artifacts and in presence of additive noise (2–20%—salt and pepper noise). The proposed approach was tested for the infrared images in the dataset and was able to successfully measure and track the ROI continuously (100% detecting and tracking the temperature of participants), and provided considerable robustness against noise (unchanged accuracy even in 20% additive noise), which shows promising performanc

Reasoning About Liquids via Closed-Loop Simulation

Simulators are powerful tools for reasoning about a robot's interactions with its environment. However, when simulations diverge from reality, that reasoning becomes less useful. In this paper, we show how to close the loop between liquid simulation and real-time perception. We use observations of liquids to correct errors when tracking the liquid's state in a simulator. Our results show that closed-loop simulation is an effective way to prevent large divergence between the simulated and real liquid states. As a direct consequence of this, our method can enable reasoning about liquids that would otherwise be infeasible due to large divergences, such as reasoning about occluded liquid.Comment: Robotics: Science & Systems (RSS), July 12-16, 2017. Cambridge, MA, US

Particulate airborne impurities

The cumulative effects of air pollutants are of principal concern in research on environmental protection in Sweden. Post-industrial society has imposed many limits on emitted air pollutants, yet the number of reports on the negative effects from them is increasing, largely due to human activity in the form of industrial emissions and increased traffic flows. Rising concerns over the health effects from airborne particulate matter (PM) stem from in vitro, in vivo, and cohort studies revealing effects of mostly negative nature. Full insight into the health effects from PM can only be achieved through practical investigation of the mode of toxicity from distinct types of particles and requires techniques for their identification, monitoring, and the production of model fractions for health studies. To this effect, comprehensive collection and chemical analysis of particulates at the origin of emission was performed in order to provide clearer insight into the nature of the particulates at exposure and add detail to aid risk assessment. Methods of capturing particles and analyzing their chemical nature were devised using scanning electron microscopy coupled with energy dispersive spectroscopy (SEM-EDS). Furthermore, taking the approach of in vitro cytotoxicity testing, nanoparticles of types typical to automotive emissions, were synthesized and extensively characterized using SEM-EDS, X-ray diffraction (XRD), transmission electron microscopy (TEM),dynamic light scattering (DLS), and nanoparticle tracking analysis (NTA). The produced model magnetite and palladium nanoparticles were found to induce toxicity in human pulmonary epithelial cells (A549 and PBEC) as well as impact severely on immunological and renal cells (221 B- and 293T-cells) in a dose-dependent manner

Unsupervised automatic tracking of thermal changes in human body

An automated system for detecting and tracking of the thermal fluctuation in human body is addressed. It applies HSV based k-means clustering which initialized and controlled the points which lie on the ROI boundary. Afterward a particle filter tracked the targeted ROI in the thermal video stream. There were six subjects have voluntarily participated on these experiments. For simulating the hot spots occur during the some medical tests a controllable heater utilized close to the subjects body. The results indicated promising accuracy of the proposed approach for tracking the hot spots. However, there were some approximations (e.g. the transmittance of the atmosphere and emissivity of the fabric) which can be neglected because of independency of the proposed approach for these parameters. The approach can track the heating spots efficiently considering the movement in the subjects which provided a confidence of considerable robustness against motion-artifact usually occurs in the medical tests

The NASA SBIR product catalog

The purpose of this catalog is to assist small business firms in making the community aware of products emerging from their efforts in the Small Business Innovation Research (SBIR) program. It contains descriptions of some products that have advanced into Phase 3 and others that are identified as prospective products. Both lists of products in this catalog are based on information supplied by NASA SBIR contractors in responding to an invitation to be represented in this document. Generally, all products suggested by the small firms were included in order to meet the goals of information exchange for SBIR results. Of the 444 SBIR contractors NASA queried, 137 provided information on 219 products. The catalog presents the product information in the technology areas listed in the table of contents. Within each area, the products are listed in alphabetical order by product name and are given identifying numbers. Also included is an alphabetical listing of the companies that have products described. This listing cross-references the product list and provides information on the business activity of each firm. In addition, there are three indexes: one a list of firms by states, one that lists the products according to NASA Centers that managed the SBIR projects, and one that lists the products by the relevant Technical Topics utilized in NASA's annual program solicitation under which each SBIR project was selected

Automated assessment and tracking of human body thermal variations using unsupervised clustering

The presented approach addresses a review of the overheating that occurs during radiological examinations, such as magnetic resonance imaging, and a series of thermal experiments to determine a thermally suitable fabric material that should be used for radiological gowns. Moreover, an automatic system for detecting and tracking of the thermal fluctuation is presented. It applies hue-saturated-value-based kernelled k-means clustering, which initializes and controls the points that lie on the region-of-interest (ROI) boundary. Afterward, a particle filter tracks the targeted ROI during the video sequence independently of previous locations of overheating spots. The proposed approach was tested during experiments and under conditions very similar to those used during real radiology exams. Six subjects have voluntarily participated in these experiments. To simulate the hot spots occurring during radiology, a controllable heat source was utilized near the subject’s body. The results indicate promising accuracy for the proposed approach to track hot spots. Some approximations were used regarding the transmittance of the atmosphere, and emissivity of the fabric could be neglected because of the independence of the proposed approach for these parameters. The approach can track the heating spots continuously and correctly, even for moving subjects, and provides considerable robustness against motion artifact, which occurs during most medical radiology procedures