7,085 research outputs found
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The Influence of Electron Beam Sterilization on In Vivo Degradation of β-TCP/PCL of Different Composite Ratios for Bone Tissue Engineering.
We evaluated the effect of electron beam (E-beam) sterilization (25 kGy, ISO 11137) on the degradation of β-tricalcium phosphate/polycaprolactone (β-TCP/PCL) composite filaments of various ratios (0:100, 20:80, 40:60, and 60:40 TCP:PCL by mass) in a rat subcutaneous model for 24 weeks. Volumes of the samples before implantation and after explantation were measured using micro-computed tomography (micro-CT). The filament volume changes before sacrifice were also measured using a live micro-CT. In our micro-CT analyses, there was no significant difference in volume change between the E-beam treated groups and non-E-beam treated groups of the same β-TCP to PCL ratios, except for the 0% β-TCP group. However, the average volume reduction differences between the E-beam and non-E-beam groups in the same-ratio samples were 0.76% (0% TCP), 3.30% (20% TCP), 4.65% (40% TCP), and 3.67% (60% TCP). The E-beam samples generally had more volume reduction in all experimental groups. Therefore, E-beam treatment may accelerate degradation. In our live micro-CT analyses, most volume reduction arose in the first four weeks after implantation and slowed between 4 and 20 weeks in all groups. E-beam groups showed greater volume reduction at every time point, which is consistent with the results by micro-CT analysis. Histology results suggest the biocompatibility of TCP/PCL composite filaments
Fabrication of micro-structures for optically driven micromachines using two-photon photopolymerization of UV curing resins
Two-photon photopolymerization of UV curing resins is an attractive method
for the fabrication of microscopic transparent objects with size in the tens of
micrometers range. We have been using this method to produce three-dimensional
structures for optical micromanipulation, in an optical system based on a
femtosecond laser. By carefully adjusting the laser power and the exposure time
we were able to create micro-objects with well-defined 3D features and with
resolution below the diffraction limit of light. We discuss the performance and
capabilities of a microfabrication system, with some examples of its products.Comment: 12 pages, 10 figure
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A Wireless Implantable System for Facilitating Gastrointestinal Motility.
Gastrointestinal (GI) electrical stimulation has been shown in several studies to be a potential treatment option for GI motility disorders. Despite the promising preliminary research progress, however, its clinical applicability and usability are still unknown and limited due to the lack of a miniaturized versatile implantable stimulator supporting the investigation of effective stimulation patterns for facilitating GI dysmotility. In this paper, we present a wireless implantable GI modulation system to fill this technology gap. The system consists of a wireless extraluminal gastrointestinal modulation device (EGMD) performing GI electrical stimulation, and a rendezvous device (RD) and a custom-made graphical user interface (GUI) outside the body to wirelessly power and configure the EGMD to provide the desired stimuli for modulating GI smooth muscle activities. The system prototype was validated in bench-top and in vivo tests. The GI modulation system demonstrated its potential for facilitating intestinal transit in the preliminary in vivo chronic study using porcine models
On-chip inverted emulsion method for fast giant vesicle production, handling, and analysis
Liposomes and giant unilamellar vesicles (GUVs) in particular are excellent compartments for constructing artificial cells. Traditionally, their use requires bench-top vesicle growth, followed by experimentation under a microscope. Such steps are time-consuming and can lead to loss of vesicles when they are transferred to an observation chamber. To overcome these issues, we present an integrated microfluidic chip which combines GUV formation, trapping, and multiple separate experiments in the same device. First, we optimized the buffer conditions to maximize both the yield and the subsequent trapping of the vesicles in micro-posts. Captured GUVs were monodisperse with specific size of 18 ± 4 µm in diameter. Next, we introduce a two-layer design with integrated valves which allows fast solution exchange in less than 20 s and on separate sub-populations of the trapped vesicles. We demonstrate that multiple experiments can be performed in a single chip with both membrane transport and permeabilization assays. In conclusion, we have developed a versatile all-in-one microfluidic chip with capabilities to produce and perform multiple experiments on a single batch of vesicles using low sample volumes. We expect this device will be highly advantageous for bottom-up synthetic biology where rapid encapsulation and visualization is required for enzymatic reactions
Ultra-Stretchable Interconnects for High-Density Stretchable Electronics
The exciting field of stretchable electronics (SE) promises numerous novel
applications, particularly in-body and medical diagnostics devices. However,
future advanced SE miniature devices will require high-density, extremely
stretchable interconnects with micron-scale footprints, which calls for proven
standardized (complementary metal-oxide semiconductor (CMOS)-type) process
recipes using bulk integrated circuit (IC) microfabrication tools and
fine-pitch photolithography patterning. Here, we address this combined
challenge of microfabrication with extreme stretchability for high-density SE
devices by introducing CMOS-enabled, free-standing, miniaturized interconnect
structures that fully exploit their 3D kinematic freedom through an interplay
of buckling, torsion, and bending to maximize stretchability. Integration with
standard CMOS-type batch processing is assured by utilizing the Flex-to-Rigid
(F2R) post-processing technology to make the back-end-of-line interconnect
structures free-standing, thus enabling the routine microfabrication of
highly-stretchable interconnects. The performance and reproducibility of these
free-standing structures is promising: an elastic stretch beyond 2000% and
ultimate (plastic) stretch beyond 3000%, with 10
million cycles at 1000% stretch with <1% resistance change. This generic
technology provides a new route to exciting highly-stretchable miniature
devices.Comment: 13 pages, 5 figure, journal publicatio
Microspinning: Local Surface Mixing via Rotation of Magnetic Microparticles for Efficient Small-Volume Bioassays
The need for high-throughput screening has led to the miniaturization of the reaction volume of the chamber in bioassays. As the reactor gets smaller, surface tension dominates the gravitational or inertial force, and mixing efficiency decreases in small-scale reactions. Because passive mixing by simple diffusion in tens of microliter-scale volumes takes a long time, active mixing is needed. Here, we report an efficient micromixing method using magnetically rotating microparticles with patterned magnetization induced by magnetic nanoparticle chains. Because the microparticles have magnetization patterning due to fabrication with magnetic nanoparticle chains, the microparticles can rotate along the external rotating magnetic field, causing micromixing. We validated the reaction efficiency by comparing this micromixing method with other mixing methods such as simple diffusion and the use of a rocking shaker at various working volumes. This method has the potential to be widely utilized in suspension assay technology as an efficient mixing strategy
Towards efficient modelling of optical micromanipulation of complex structures
Computational methods for electromagnetic and light scattering can be used
for the calculation of optical forces and torques. Since typical particles that
are optically trapped or manipulated are on the order of the wavelength in
size, approximate methods such as geometric optics or Rayleigh scattering are
inapplicable, and solution or either the Maxwell equations or the vector
Helmholtz equation must be resorted to. Traditionally, such solutions were only
feasible for the simplest geometries; modern computational power enable the
rapid solution of more general--but still simple--geometries such as
axisymmetric, homogeneous, and isotropic scatterers. However, optically-driven
micromachines necessarily require more complex geometries, and their
computational modelling thus remains in the realm of challenging computational
problems. We review our progress towards efficient computational modelling of
optical tweezers and micromanipulation, including the trapping and manipulation
of complex structures such as optical micromachines. In particular, we consider
the exploitation of symmetry in the modelling of such devices.Comment: 5 pages, 4 figure
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Towards On-Chip Self-Referenced Frequency-Comb Sources Based on Semiconductor Mode-Locked Lasers.
Miniaturization of frequency-comb sources could open a host of potential applications in spectroscopy, biomedical monitoring, astronomy, microwave signal generation, and distribution of precise time or frequency across networks. This review article places emphasis on an architecture with a semiconductor mode-locked laser at the heart of the system and subsequent supercontinuum generation and carrier-envelope offset detection and stabilization in nonlinear integrated optics
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