Microfluidics for effective concentration and sorting of waterborne protozoan pathogens

We report on an inertial focussing based microfluidics technology for concentrating waterborne protozoa, achieving a 96% recovery rate of Cryptosporidium parvum and 86% for Giardia lamblia at a throughput (mL/min) capable of replacing centrifugation. The approach can easily be extended to other parasites and also bacteria

Microfluidics: an enabling technology for the life sciences

During the last year we have investigated existing and future markets, products and technologies for microfluidics in the life sciences. Within this paper we present some of the findings and discuss a major trend identified within this project: the development of microfluidic platforms for flexible design of application specific integrated microfluidic systems. We discuss two platforms in detail which are currently under development in our lab: microfluidics on a rotating CD ("Lab-CD") as well as a platform to realized customized "nanoliter & picoliter dispensing systems"

Testing microelectronic biofluidic systems

According to the 2005 International Technology Roadmap for Semiconductors, the integration of emerging nondigital CMOS technologies will require radically different test methods, posing a major challenge for designers and test engineers. One such technology is microelectronic fluidic (MEF) arrays, which have rapidly gained importance in many biological, pharmaceutical, and industrial applications. The advantages of these systems, such as operation speed, use of very small amounts of liquid, on-board droplet detection, signal conditioning, and vast digital signal processing, make them very promising. However, testable design of these devices in a mass-production environment is still in its infancy, hampering their low-cost introduction to the market. This article describes analog and digital MEF design and testing method

Mithramycin encapsulated in polymeric micelles by microfluidic technology as novel therapeutic protocol for beta-thalassemia

This report shows that the DNA-binding drug, mithramycin, can be efficiently encapsulated in polymeric micelles (PM-MTH), based on Pluronic® block copolymers, by a new microfluidic approach. The effect of different production parameters has been investigated for their effect on PM-MTH characteristics. The compared analysis of PM-MTH produced by microfluidic and conventional bulk mixing procedures revealed that microfluidics provides a useful platform for the production of PM-MTH with improved controllability, reproducibility, smaller size, and polydispersity. Finally, an investigation of the effects of PM-MTH, produced by microfluidic and conventional bulk mixing procedures, on the erythroid differentiation of both human erythroleukemia and human erythroid precursor cells is reported. It is demonstrated that PM-MTH exhibited a slightly lower toxicity and more pronounced differentiative activity when compared to the free drug. In addition, PM-MTH were able to upregulate preferentially ?-globin messenger ribonucleic acid production and to increase fetal hemoglobin (HbF) accumulation, the percentage of HbF-containing cells, and their HbF content without stimulating ?-globin gene expression, which is responsible for the clinical symptoms of ß-thalassemia. These results represent an important first step toward a potential clinical application, since an increase in HbF could alleviate the symptoms underlying ß-thalassemia and sickle cell anemia. In conclusion, this report suggests that PM-MTH produced by microfluidic approach warrants further evaluation as a potential therapeutic protocol for ß-thalassemia.<br/

Advances in Microfluidics and Lab-on-a-Chip Technologies

Advances in molecular biology are enabling rapid and efficient analyses for effective intervention in domains such as biology research, infectious disease management, food safety, and biodefense. The emergence of microfluidics and nanotechnologies has enabled both new capabilities and instrument sizes practical for point-of-care. It has also introduced new functionality, enhanced sensitivity, and reduced the time and cost involved in conventional molecular diagnostic techniques. This chapter reviews the application of microfluidics for molecular diagnostics methods such as nucleic acid amplification, next-generation sequencing, high resolution melting analysis, cytogenetics, protein detection and analysis, and cell sorting. We also review microfluidic sample preparation platforms applied to molecular diagnostics and targeted to sample-in, answer-out capabilities

A reverse predictive model towards design automation of microfluidic droplet generators

This work has been presented in the 10th IWBDA workshop.Droplet-based microfluidic devices in comparison to test tubes can reduce reaction volumes 10^9 times and more due to the encapsulation of reactions in micro-scale droplets [4]. This volume reduction, alongside higher accuracy, higher sensitivity and faster reaction time made droplet microfluidics a superior platform particularly in biology, biomedical, and chemical engineering. However, a high barrier of entry prevents most of life science laboratories to exploit the advantages of microfluidics. There are two main obstacles to the widespread adoption of microfluidics, high fabrication costs, and lack of design automation tools. Recently, low-cost fabrication methods have reduced the cost of fabrication significantly [7]. Still, even with a low-cost fabrication method, due to lack of automation tools, life science research groups are still reliant on a microfluidic expert to develop any new microfluidic device [3, 5]. In this work, we report a framework to develop reverse predictive models that can accurately automate the design process of microfluidic droplet generators. This model takes prescribed performance metrics of droplet generators as the input and provides the geometry of the microfluidic device and the fluid and flow settings that result in the desired performance. We hope this automation tool makes droplet-based microfluidics more accessible, by reducing the time, cost, and knowledge needed for developing a microfluidic droplet generator that meets certain performance requirement

Microfluidic multipoles: theory and applications

Microfluidic multipoles (MFMs) have been realized experimentally and hold promise for "open-space" biological and chemical surface processing. Whereas convective flow can readily be predicted using hydraulic-electrical analogies, the design of advanced MFMs is constrained by the lack of simple, accurate models to predict mass transport within them. In this work, we introduce the first exact solutions to mass transport in multipolar microfluidics based on the iterative conformal mapping of 2D advection-diffusion around a simple edge into dipoles and multipolar geometries, revealing a rich landscape of transport modes. The models were validated experimentally with a library of 3D printed MFM devices and found in excellent agreement. Following a theory-guided design approach, we further ideated and fabricated two new classes of spatiotemporally reconfigurable MFM devices that are used for processing surfaces with time-varying reagent streams, and to realize a multistep automated immunoassay. Overall, the results set the foundations for exploring, developing, and applying open-space MFMs.Comment: 16 pages, 5 figure

Molecular dynamics studies of anomalous transport in rarefied gas flows

We investigate the thermodynamically non-equilibrium gas dynamics by measuring molecular free path distribution functions, inter-molecular collision rates and wall dependent mean free path (MFP) profiles using the molecular dynamics (MD) method. The simulations cover a wide range of fluid densities for single-wall case, parallel walls cases and a cube with all periodic walls. The simulations are validated by deducing the theoretical unconfined MFP values at standardpressure and temperature conditions. The free path MD measurements of individual molecules convey that conventional exponential distribution function is not valid under rarefied conditions and molecules follow L´evy type flights, irrespective of the presence of a wall. MFP profile measurements for confined planar surfaces in the transition flow regime show sharp gradients close to the wall, while theoretical models predict shallower gradients. As gas transport properties can be related to the MFP through kinetic theory, our MD data may help to modify the constitutive relationships, which may then be fed into the Navier-Stokes equations for better effective modeling of micro gas flows in the transition flow regime

Microfluidics for Energy Applications

This paper was presented at the 4th Micro and Nano Flows Conference (MNF2014), which was held at University College, London, UK. The conference was organised by Brunel University and supported by the Italian Union of Thermofluiddynamics, IPEM, the Process Intensification Network, the Institution of Mechanical Engineers, the Heat Transfer Society, HEXAG - the Heat Exchange Action Group, and the Energy Institute, ASME Press, LCN London Centre for Nanotechnology, UCL University College London, UCL Engineering, the International NanoScience Community, www.nanopaprika.eu.Microfluidic methods developed primarily for medical applications have much to offer energy applications. This short paper will provide the motivation and outline my group’s recent work in two such areas: (1) microfluidics and optics for bioenergy and (2) microfluidics for carbon management. Full details will be provided in talk. Within the bioenergy theme, we are developing photobioreactor architectures that leverage micro-optics and microfluidics to cater both light and fluids to maximize productivity of microalgae. Within the carbon management theme we are developing a suite of methods to study porescale transport and reactivity in carbon sequestration and enhanced oil recovery. Results indicate potential for order of magnitude gains in photobioreactor technology and a 100-fold improvement over current subsurface fluid transport analysis methods