1,618 research outputs found
Mitigating the Multipath Effects on Radio Tomographic Imaging
Various radio tomographic imaging (RTI) models and reconstruction methods are equipped with capabilities to mitigate the effects of multipath interference. This thesis combined the network shadowing (NeSh) and weighting-g models in conjunction with Tikhonov regularization and low-rank and sparse decomposition (LRSD). MATLAB was used to implement the four combinations for six experimental data sets and produce attenuation images. The attenuation images were analyzed qualitatively and quantitatively to accomplish the goal of determining which combination performed best at locating human targets. After analyzing the results, it was determined that no single combination outperformed the others for at least three out of the five quantitative metrics. Therefore, a rating technique was used instead to normalize the average results of each metric and find the mean across each combination\u27s newly normalized average results. In accordance with the normalization scale, the lowest and best rating revealed the optimum combination was the weighting-g model implemented in conjunction with LRSD
Nonlinear two-dimensional terahertz photon echo and rotational spectroscopy in the gas phase
Ultrafast two-dimensional spectroscopy utilizes correlated multiple
light-matter interactions for retrieving dynamic features that may otherwise be
hidden under the linear spectrum. Its extension to the terahertz regime of the
electromagnetic spectrum, where a rich variety of material degrees of freedom
reside, remains an experimental challenge. Here we report ultrafast
two-dimensional terahertz spectroscopy of gas-phase molecular rotors at room
temperature. Using time-delayed terahertz pulse pairs, we observe photon echoes
and other nonlinear signals resulting from molecular dipole orientation induced
by three terahertz field-dipole interactions. The nonlinear time-domain
orientation signals are mapped into the frequency domain in two-dimensional
rotational spectra which reveal J-state-resolved nonlinear rotational dynamics.
The approach enables direct observation of correlated rotational transitions
and may reveal rotational coupling and relaxation pathways in the ground
electronic and vibrational state.Comment: 31 pages, 14 figure
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Lensfree computational microscopy tools for cell and tissue imaging at the point-of-care and in low-resource settings.
The recent revolution in digital technologies and information processing methods present important opportunities to transform the way optical imaging is performed, particularly toward improving the throughput of microscopes while at the same time reducing their relative cost and complexity. Lensfree computational microscopy is rapidly emerging toward this end, and by discarding lenses and other bulky optical components of conventional imaging systems, and relying on digital computation instead, it can achieve both reflection and transmission mode microscopy over a large field-of-view within compact, cost-effective and mechanically robust architectures. Such high throughput and miniaturized imaging devices can provide a complementary toolset for telemedicine applications and point-of-care diagnostics by facilitating complex and critical tasks such as cytometry and microscopic analysis of e.g., blood smears, Pap tests and tissue samples. In this article, the basics of these lensfree microscopy modalities will be reviewed, and their clinically relevant applications will be discussed
High-resolution transport-of-intensity quantitative phase microscopy with annular illumination
For quantitative phase imaging (QPI) based on transport-of-intensity equation
(TIE), partially coherent illumination provides speckle-free imaging,
compatibility with brightfield microscopy, and transverse resolution beyond
coherent diffraction limit. Unfortunately, in a conventional microscope with
circular illumination aperture, partial coherence tends to diminish the phase
contrast, exacerbating the inherent noise-to-resolution tradeoff in TIE
imaging, resulting in strong low-frequency artifacts and compromised imaging
resolution. Here, we demonstrate how these issues can be effectively addressed
by replacing the conventional circular illumination aperture with an annular
one. The matched annular illumination not only strongly boosts the phase
contrast for low spatial frequencies, but significantly improves the practical
imaging resolution to near the incoherent diffraction limit. By incorporating
high-numerical aperture (NA) illumination as well as high-NA objective, it is
shown, for the first time, that TIE phase imaging can achieve a transverse
resolution up to 208 nm, corresponding to an effective NA of 2.66. Time-lapse
imaging of in vitro Hela cells revealing cellular morphology and subcellular
dynamics during cells mitosis and apoptosis is exemplified. Given its
capability for high-resolution QPI as well as the compatibility with widely
available brightfield microscopy hardware, the proposed approach is expected to
be adopted by the wider biology and medicine community.Comment: This manuscript was originally submitted on 20 Feb. 201
60-MHz wavelength-encoded tomography (WET)
Paper JW2A.82Wavelength-encoded tomography (WET) is upgraded to a triple-time-lens system to perform ultrafast cross-sectional imaging through 68.4x-temporal magnification. 60-MHz A-scan rate is demonstrated by imaging a glass sample with 180-μm axial resolution. © 2015 OSApostprin
Emulation of X-ray Light-Field Cameras
X-ray plenoptic cameras acquire multi-view X-ray transmission images in a single exposure (light-field). Their development is challenging: designs have appeared only recently, and they are still affected by important limitations. Concurrently, the lack of available real X-ray light-field data hinders dedicated algorithmic development. Here, we present a physical emulation setup for rapidly exploring the parameter space of both existing and conceptual camera designs. This will assist and accelerate the design of X-ray plenoptic imaging solutions, and provide a tool for generating unlimited real X-ray plenoptic data. We also demonstrate that X-ray light-fields allow for reconstructing sharp spatial structures in three-dimensions (3D) from single-shot data
Correlative cellular ptychography with functionalized nanoparticles at the Fe L-edge
Precise localization of nanoparticles within a cell is crucial to the understanding of cell-particle interactions and has broad applications in nanomedicine. Here, we report a proof-of-principle experiment for imaging individual functionalized nanoparticles within a mammalian cell by correlative microscopy. Using a chemically-fixed HeLa cell labeled with fluorescent core-shell nanoparticles as a model system, we implemented a graphene-oxide layer as a substrate to significantly reduce background scattering. We identified cellular features of interest by fluorescence microscopy, followed by scanning transmission X-ray tomography to localize the particles in 3D, and ptychographic coherent diffractive imaging of the fine features in the region at high resolution. By tuning the X-ray energy to the Fe L-edge, we demonstrated sensitive detection of nanoparticles composed of a 22 nm magnetic FeO core encased by a 25-nm-thick fluorescent silica (SiO) shell. These fluorescent core-shell nanoparticles act as landmarks and offer clarity in a cellular context. Our correlative microscopy results confirmed a subset of particles to be fully internalized, and high-contrast ptychographic images showed two oxidation states of individual nanoparticles with a resolution of ~16.5 nm. The ability to precisely localize individual fluorescent nanoparticles within mammalian cells will expand our understanding of the structure/function relationships for functionalized nanoparticles
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