Love Wave Immunosensor for the Detection of Carbaryl Pesticide

A Love Wave (LW) immunosensor was developed for the detection of carbaryl pesticide. The experimental setup consisted on: a compact electronic characterization circuit based on phase and amplitude detection at constant frequency; an automated flow injection system; a thermal control unit; a custom-made flow-through cell; and Quartz /SiO2 LW sensors with a 40 μm wavelength and 120 MHz center frequency. The carbaryl detection was based on a competitive immunoassay format using LIB-CNH45 monoclonal antibody (MAb). Bovine Serum Albumin-CNH (BSA-CNH) carbaryl hapten-conjugate was covalently immobilized, via mercaptohexadecanoic acid self-assembled monolayer (SAM), onto the gold sensing area of the LW sensors. This immobilization allowed the reusability of the sensor for at least 70 assays without significant signal losses. The LW immunosensor showed a limit of detection (LOD) of 0.09 μg/L, a sensitivity of 0.31 μg/L and a linear working range of 0.14–1.63 μg/L. In comparison to other carbaryl immunosensors, the LW immunosensor achieved a high sensitivity and a low LOD. These features turn the LW immunosensor into a promising tool for applications that demand a high resolution, such as for the detection of pesticides in drinking water at European regulatory levels.The author also would like to acknowledge the Spanish Ministry of Economy and Competitiveness and the European Regional Development Fund (ERDF) for their financing support through the grant of the INNPACTO 2012 project (DETECTA IPT-2012-0154-300000), J.V. Garcia's Fellowship, AP2007-03745, of FPU (Formacion de Profesorado Universitario) program, M. I. Rocha-Gaso's PhD CONACyT Fellowship and AWSensors Inc. for its collaboration.Rocha-Gaso, M.; García Narbón, JV.; García, P.; March Iborra, MDC.; Jiménez Jiménez, Y.; Francis, L.; Montoya Baides, Á.... (2014). Love Wave Immunosensor for the Detection of Carbaryl Pesticide. Sensors. 14(9):16434-16453. https://doi.org/10.3390/s140916434S1643416453149March, C., Manclús, J. J., Jiménez, Y., Arnau, A., & Montoya, A. (2009). A piezoelectric immunosensor for the determination of pesticide residues and metabolites in fruit juices. Talanta, 78(3), 827-833. doi:10.1016/j.talanta.2008.12.058Janshoff, A., Galla, H.-J., & Steinem, C. (2000). Piezoelectric Mass-Sensing Devices as Biosensors—An Alternative to Optical Biosensors? Angewandte Chemie, 39(22), 4004-4032. doi:10.1002/1521-3773(20001117)39:223.0.co;2-2Rocha-Gaso, M.-I., March-Iborra, C., Montoya-Baides, Á., & Arnau-Vives, A. (2009). Surface Generated Acoustic Wave Biosensors for the Detection of Pathogens: A Review. Sensors, 9(7), 5740-5769. doi:10.3390/s90705740Gronewold, T. M. A. (2007). Surface acoustic wave sensors in the bioanalytical field: Recent trends and challenges. Analytica Chimica Acta, 603(2), 119-128. doi:10.1016/j.aca.2007.09.056Länge, K., Rapp, B. E., & Rapp, M. (2008). Surface acoustic wave biosensors: a review. Analytical and Bioanalytical Chemistry, 391(5), 1509-1519. doi:10.1007/s00216-008-1911-5Arnau, A., Montagut, Y., García, J. V., & Jiménez, Y. (2009). A different point of view on the sensitivity of quartz crystal microbalance sensors. Measurement Science and Technology, 20(12), 124004. doi:10.1088/0957-0233/20/12/124004Montagut, Y. J., García, J. V., Jiménez, Y., March, C., Montoya, A., & Arnau, A. (2011). Frequency-shift vs phase-shift characterization of in-liquid quartz crystal microbalance applications. Review of Scientific Instruments, 82(6), 064702. doi:10.1063/1.3598340Abad, A., Primo, J., & Montoya, A. (1997). Development of an Enzyme-Linked Immunosorbent Assay to Carbaryl. 1. Antibody Production from Several Haptens and Characterization in Different Immunoassay Formats. Journal of Agricultural and Food Chemistry, 45(4), 1486-1494. doi:10.1021/jf9506904Manclús, J. J., Abad, A., Lebedev, M. Y., Mojarrad, F., Micková, B., Mercader, J. V., … Montoya, A. (2004). Development of a Monoclonal Immunoassay Selective for Chlorinated Cyclodiene Insecticides. Journal of Agricultural and Food Chemistry, 52(10), 2776-2784. doi:10.1021/jf035382hFrancis, L. A., Friedt, J.-M., Zhou, C., & Bertrand, P. (2006). In Situ Evaluation of Density, Viscosity, and Thickness of Adsorbed Soft Layers by Combined Surface Acoustic Wave and Surface Plasmon Resonance. Analytical Chemistry, 78(12), 4200-4209. doi:10.1021/ac051233hMauriz, E., Calle, A., Abad, A., Montoya, A., Hildebrandt, A., Barceló, D., & Lechuga, L. M. (2006). Determination of carbaryl in natural water samples by a surface plasmon resonance flow-through immunosensor. Biosensors and Bioelectronics, 21(11), 2129-2136. doi:10.1016/j.bios.2005.10.013Abad, A., & Montoya, A. (1997). Development of an Enzyme-Linked Immunosorbent Assay to Carbaryl. 2. Assay Optimization and Application to the Analysis of Water Samples. Journal of Agricultural and Food Chemistry, 45(4), 1495-1501. doi:10.1021/jf950691wMontagut, Y., García, J. V., Jiménez, Y., March, C., Montoya, Á., & Arnau, A. (2011). Validation of a Phase-Mass Characterization Concept and Interface for Acoustic Biosensors. Sensors, 11(5), 4702-4720. doi:10.3390/s11050470

Lab-on-a-chip based integrated hybrid technologies for biofluids manipulation and characterization

[EN] The goal of this work is to develop an original and high performance hybrid lab-on-a-chip, coupling actuators and biosensors for the active control and broad range characterization of biofluids samples. These biofluids will be controlled (moved and mixed) actively by Rayleigh-Surface Acoustic Wave (R-SAW) and analysed in real-time by combining Love-SAW (L-SAW) and Surface Plasmon Resonance (SPR) technologies. A microfluidic chamber and specific transducer were designed for this application to interact efficiently with the liquid sample entrapped in the chamber and to detect modifications of its physical properties such as viscosity. AT-cut quartz and LiNbO3 36Y-X substrates were used to generate both Rayleigh and Shear-Horizontal waves and ZnO material as guiding layer. The whole system has proven is full efficiency to interact with the fluid and to detect the signal perturbations with no significant loss compared with the measurements carried out with a probe station.The authors wish to thank the ANR for its financial support through the AWESOM project (ANR-12-BS09-021); the operators Laurent Bouvot, Jean Georges Mussot and Emmanuel Vatoux, for their support and assistance in the fabrication of the cell and the SAW devices; and the engineering students, Olivier Bettoni and Bastien Lafont, for their contribution to the cell design.Rocha Gaso, MI.; Renaudin, A.; Sarry, F.; Beyssen, D. (2015). Lab-on-a-chip based integrated hybrid technologies for biofluids manipulation and characterization. Procedia Engineering. 120:687-690. https://doi.org/10.1016/j.proeng.2015.08.75068769012

Properties and Applications of Love Surface Waves in Seismology and Biosensors

Shear horizontal (SH) surface waves of the Love type are elastic surface waves propagating in layered waveguides, in which surface layer is “slower” than the substrate. Love surface waves are of primary importance in geophysics and seismology, since most structural damages in the wake of earthquakes are attributed to the devastating SH motion inherent to the Love surface waves. On the other hand, Love surface waves found benign applications in biosensors used in biology, medicine, and chemistry. In this chapter, we briefly sketch a mathematical model for Love surface waves and present examples of the resulting dispersion curves for phase and group velocities, attenuation as well as the amplitude distribution as a function of the depth. We illustrate damages due to Love surface waves generated by earthquakes on real-life examples. In the following of this chapter, we present a number of representative examples for Love wave biosensors, which have been already used to DNA characterization, bacteria and virus detection, measurements of toxic substances, etc. We hope that the reader, after studying this chapter, will have a clear idea that deadly earthquakes and a beneficiary biosensor technology share the same physical phenomenon, which is the basis of a fascinating interdisciplinary research