Maskless imaging of dense samples using pixel super-resolution based multi-height lensfree on-chip microscopy.

Lensfree in-line holographic microscopy offers sub-micron resolution over a large field-of-view (e.g., ~24 mm2) with a cost-effective and compact design suitable for field use. However, it is limited to relatively low-density samples. To mitigate this limitation, we demonstrate an on-chip imaging approach based on pixel super-resolution and phase recovery, which iterates among multiple lensfree intensity measurements, each having a slightly different sample-to-sensor distance. By digitally aligning and registering these lensfree intensity measurements, phase and amplitude images of dense and connected specimens can be iteratively reconstructed over a large field-of-view of ~24 mm2 without the use of any spatial masks. We demonstrate the success of this multi-height in-line holographic approach by imaging dense Papanicolaou smears (i.e., Pap smears) and blood samples

Vision-based Real-Time Aerial Object Localization and Tracking for UAV Sensing System

The paper focuses on the problem of vision-based obstacle detection and tracking for unmanned aerial vehicle navigation. A real-time object localization and tracking strategy from monocular image sequences is developed by effectively integrating the object detection and tracking into a dynamic Kalman model. At the detection stage, the object of interest is automatically detected and localized from a saliency map computed via the image background connectivity cue at each frame; at the tracking stage, a Kalman filter is employed to provide a coarse prediction of the object state, which is further refined via a local detector incorporating the saliency map and the temporal information between two consecutive frames. Compared to existing methods, the proposed approach does not require any manual initialization for tracking, runs much faster than the state-of-the-art trackers of its kind, and achieves competitive tracking performance on a large number of image sequences. Extensive experiments demonstrate the effectiveness and superior performance of the proposed approach.Comment: 8 pages, 7 figure

A quantitative image analysis pipeline for the characterization of filamentous fungal morphologies as a tool to uncover targets for morphology engineering: a case study using aplD in Aspergillus niger

Background Fungal fermentation is used to produce a diverse repertoire of enzymes, chemicals, and drugs for various industries. During submerged cultivation, filamentous fungi form a range of macromorphologies, including dispersed mycelia, clumped aggregates, or pellets, which have critical implications for rheological aspects during fermentation, gas/nutrient transfer, and, thus, product titres. An important component of strain engineering efforts is the ability to quantitatively assess fungal growth phenotypes, which will drive novel leads for morphologically optimized production strains. Results In this study, we developed an automated image analysis pipeline to quantify the morphology of pelleted and dispersed growth (MPD) which rapidly and reproducibly measures dispersed and pelleted macromorphologies from any submerged fungal culture. It (i) enables capture and analysis of several hundred images per user/day, (ii) is designed to quantitatively assess heterogeneous cultures consisting of dispersed and pelleted forms, (iii) gives a quantitative measurement of culture heterogeneity, (iv) automatically generates key Euclidian parameters for individual fungal structures including particle diameter, aspect ratio, area, and solidity, which are also assembled into a previously described dimensionless morphology number MN, (v) has an in-built quality control check which enables end-users to easily confirm the accuracy of the automated calls, and (vi) is easily adaptable to user-specified magnifications and macromorphological definitions. To concomitantly provide proof of principle for the utility of this image analysis pipeline, and provide new leads for morphologically optimized fungal strains, we generated a morphological mutant in the cell factory Aspergillus niger based on CRISPR-Cas technology. First, we interrogated a previously published co-expression networks for A. niger to identify a putative gamma-adaptin encoding gene (aplD) that was predicted to play a role in endosome cargo trafficking. Gene editing was used to generate a conditional aplD expression mutant under control of the titratable Tet-on system. Reduced aplD expression caused a hyperbranched growth phenotype and diverse defects in pellet formation with a putative increase in protein secretion. This possible protein hypersecretion phenotype could be correlated with increased dispersed mycelia, and both decreased pellet diameter and MN. Conclusion The MPD image analysis pipeline is a simple, rapid, and flexible approach to quantify diverse fungal morphologies. As an exemplar, we have demonstrated that the putative endosomal transport gene aplD plays a crucial role in A. niger filamentous growth and pellet formation during submerged culture. This suggests that endocytic components are underexplored targets for engineering fungal cell factories.DFG, 414044773, Open Access Publizieren 2019 - 2020 / Technische Universität Berli

High Quality 3D Shape Reconstruction via Digital Refocusing and Pupil Apodization in Multi-wavelength Holographic Interferometry.

Multi-wavelength holographic interferometry (MWHI) has good potential for evolving into a high quality 3D shape reconstruction technique. There are several remaining challenges, including 1) depth-of-field limitation, leading to axial dimension inaccuracy of out-of-focus objects; and 2) smearing from shiny smooth objects to their dark dull neighbors, generating fake measurements within the dark area. This research is motivated by the goal of developing an advanced optical metrology system that provides accurate 3D profiles for target object or objects of axial dimension larger than the depth-of-field, and for objects with dramatically different surface conditions. The idea of employing digital refocusing in MWHI has been proposed as a solution to the depth-of-field limitation. One the one hand, traditional single wavelength refocusing formula is revised to reduce sensitivity to wavelength error. Investigation over real example demonstrates promising accuracy and repeatability of reconstructed 3D profiles. On the other hand, a phase contrast based focus detection criterion is developed especially for MWHI, which overcomes the problem of phase unwrapping. The combination for these two innovations gives birth to a systematic strategy of acquiring high quality 3D profiles. Following the first phase contrast based focus detection step, interferometric distance measurement by MWHI is implemented as a next step to conduct relative focus detection with high accuracy. This strategy results in ±100mm 3D profile with micron level axial accuracy, which is not available in traditional extended focus image (EFI) solutions. Pupil apodization has been implemented to address the second challenge of smearing. The process of reflective rough surface inspection has been mathematically modeled, which explains the origin of stray light and the necessity of replacing hard-edged pupil with one of gradually attenuating transmission (apodization). Metrics to optimize pupil types and parameters have been chosen especially for MWHI. A Gaussian apodized pupil has been installed and tested. A reduction of smearing in measurement result has been experimentally demonstrated.Ph.D.Mechanical EngineeringUniversity of Michigan, Horace H. Rackham School of Graduate Studieshttp://deepblue.lib.umich.edu/bitstream/2027.42/91461/1/xulium_1.pd

Exploratory Study of Service Quality, Corporate Image, and Customer Loyalty in Restaurants in Ghana

The aim of the study is to identify the effect of Service Quality and Corporate Image on Customer’s Loyalty .Questionnaires were distributed to collect responses from restaurant users in the Greater Accra region of Ghana. Descriptive statistics and correlation analysis were used to analyze the data and draw the conclusions. It was revealed that service quality and corporate image have strong positive association with customer’s loyalty .The study suggested that managers of restaurants provide needed logistics to aid frontline staff to avoid delays in meeting customer demands. This will lead to high level of customer retention, loyalty, high market share and profitability

Selection of the key earth observation sensors and platforms focusing on applications for Polar Regions in the scope of Copernicus system 2020-2030

An optimal payload selection conducted in the frame of the H2020 ONION project (id 687490) is presented based on the ability to cover the observation needs of the Copernicus system in the time period 2020–2030. Payload selection is constrained by the variables that can be measured, the power consumption, and weight of the instrument, and the required accuracy and spatial resolution (horizontal or vertical). It involved 20 measurements with observation gaps according to the user requirements that were detected in the top 10 use cases in the scope of Copernicus space infrastructure, 9 potential applied technologies, and 39 available commercial platforms. Additional Earth Observation (EO) infrastructures are proposed to reduce measurements gaps, based on a weighting system that assigned high relevance for measurements associated to Marine for Weather Forecast over Polar Regions. This study concludes with a rank and mapping of the potential technologies and the suitable commercial platforms to cover most of the requirements of the top ten use cases, analyzing the Marine for Weather Forecast, Sea Ice Monitoring, Fishing Pressure, and Agriculture and Forestry: Hydric stress as the priority use cases.Peer ReviewedPostprint (published version

The Profiling Potential of Computer Vision and the Challenge of Computational Empiricism

Computer vision and other biometrics data science applications have commenced a new project of profiling people. Rather than using 'transaction generated information', these systems measure the 'real world' and produce an assessment of the 'world state' - in this case an assessment of some individual trait. Instead of using proxies or scores to evaluate people, they increasingly deploy a logic of revealing the truth about reality and the people within it. While these profiling knowledge claims are sometimes tentative, they increasingly suggest that only through computation can these excesses of reality be captured and understood. This article explores the bases of those claims in the systems of measurement, representation, and classification deployed in computer vision. It asks if there is something new in this type of knowledge claim, sketches an account of a new form of computational empiricism being operationalised, and questions what kind of human subject is being constructed by these technological systems and practices. Finally, the article explores legal mechanisms for contesting the emergence of computational empiricism as the dominant knowledge platform for understanding the world and the people within it