Real-time imaging of pulvinus bending in Mimosa pudica

Mimosa pudica is a plant that rapidly shrinks its body in response to external stimuli. M. pudica does not perform merely simple movements, but exhibits a variety of movements that quickly change depending on the type of stimuli. Previous studies have investigated the motile mechanism of the plants from a biochemical perspective. However, an interdisciplinary study on the structural characteristics of M. pudica should be accompanied by biophysical research to explain the principles underlying such movements. In this study, the structural characteristics and seismonastic reactions of M. pudica were experimentally investigated using advanced bio-imaging techniques. The results show that the key factors for the flexible movements by the pulvinus are the following: bendable xylem bundle, expandable/shrinkable epidermis, tiny wrinkles for surface modification, and a xylem vessel network for efficient water transport. This study provides new insight for better understanding the M. pudica motile mechanism through structural modification.open1111Nsciescopu

Real-time imaging of pulvinus bending in Mimosa pudica

1

Advancing interactive systems with liquid crystal network-based adaptive electronics

Achieving adaptive behavior in artificial systems, analogous to living organisms, has been a long-standing goal in electronics and materials science. Efforts to integrate adaptive capabilities into synthetic electronics traditionally involved a typical architecture comprising of sensors, an external controller, and actuators constructed from multiple materials. However, challenges arise when attempting to unite these three components into a single entity capable of independently coping with dynamic environments. Here, we unveil an adaptive electronic unit based on a liquid crystal polymer that seamlessly incorporates sensing, signal processing, and actuating functionalities. The polymer forms a film that undergoes anisotropic deformations when exposed to a minor heat pulse generated by human touch. We integrate this property into an electric circuit to facilitate switching. We showcase the concept by creating an interactive system that features distributed information processing including feedback loops and enabling cascading signal transmission across multiple adaptive units. This system responds progressively, in a multi-layered cascade to a dynamic change in its environment. The incorporation of adaptive capabilities into a single piece of responsive material holds immense potential for expediting progress in next-generation flexible electronics, soft robotics, and swarm intelligence.</p

Identifying Molecular Functions of Heliotropic Motor Tissue Through Proteomic Analysis of Soybean Pulvini

Heliotropic and nyctinastic leaf movement are mediated in soybean through turgor changes in the motor cells of the pulvinus, located at the base of the leaves. While some elements of the signaling pathways have been studied, a broad-scale protein identification has not yet been reported. In my research pulvini proteins were extracted in light- and dark-harvested soybean using the TCA/acetone method and identified by LC-MS/MS. Gene ontology analysis revealed proteins involved in proton transport were enriched in the soybean pulvinus proteome compared to a background soybean proteome. Proteins more highly expressed in the light were mostly stress response proteins, while under-expressed proteins were categorized as energy proteins. Further investigations using more sensitive extraction protocols and a multitude of sample times will build on these initial results to provide a thorough examination of heliotropic mechanisms at the molecular level

The Smart Plant: A Look into the Controversy Behind Plant Intelligence

Plants have long been excluded from the conversation regarding intelligent functioning in living things. This mindset dates back to ancient times, when plants were assigned a low-functioning and unintelligent rung on the scala naturae. In comparison to animals, plants have evolved to respond to their environment with a modular body plan, which lacks a nervous system and ‘intelligent’ organ, such as a brain. Despite this, research has demonstrated that plants are able to sense their environment, transmit sensory information throughout the entire organism, and respond to this sensory information with appropriate physiological responses. Also, plants have been shown to demonstrate aspects of learning and memory -cognitive functions once thought to be restricted to ‘intelligent’ beings (i.e. animals). The argument against plant intelligence is largely semantic-based, and stems from the concept that the word ‘intelligence’ cannot be applied to organisms which lack organs responsible for intelligent functioning. To truly appreciate the intelligent functioning of plants, we must eliminate this semantic barrier through a re-evaluation of our conventional understanding of intelligence. Perhaps this would require us to view intelligence, not as a quality unique to animals, but as a biological property, which in varying degrees is present in all life forms

X-Ray Microanalysis of Diffusible Elements in Plant Cells After Freeze-Drying, Pressure-Infiltration with Ether and Embedding in Plastic

The technique of vacuum-pressure infiltration of freeze-dried plant tissues with diethyl ether and plastic, originally developed for the cellular localization of water-soluble 14C-assimilates, proved to be suitable for X-ray microanalysis of diffusible elements at the sub-cellular level. Apart from movements of elements caused by ice crystal formation and collapse of eutectic structures several lines of evidence suggest that additional dislocations of elements during the preparation were minimal: (1) Soluble Ca remained evenly distributed in vacuoles, (2) the contents of K relative to Ca were the same at different sites within a vacuole, (3) the relative vacuolar Ca-contents of different leaves, determined by X-ray microanalysis, corresponded to the relative Ca-contents of pressed saps of the same leaves as analyzed by atomic absorption spectrometry

Conversational ecologies

This project takes a transdisciplinary approach to spatial interactivity, incorporating elements of theoretical discourse, speculative design, narrative worldbuilding, making, scientific experimentation and video. To me it is destructive to segregate bodies of knowledge, or any bodies for that matter, and it denies the synergism that is possible with transdisciplinary work. I combine scientific materiality with imagined alechemies and interweave these throughout the text with borrowed and original philosophical contemplations to more fully grapple with the shifting complexities of Conversational Ecologies. I firmly believe that due to the complex, multisensorial nature of interactivity, the discourse must exist outside of just the written. This discourse can exist simultaneously as fantasy and reality–as long as it engages the senses and encourages people to reconsider their ecological positionalities. This theoretical, textual body acts as both a beginning for these experiments, and as a site to re-incorporate what I learn ‘in the field.

a simple tool for the analysis of periodic cotyledon and leaf movement in Arabidopsis thaliana

Background The analysis of circadian leaf movement rhythms is a simple yet effective method to study effects of treatments or gene mutations on the circadian clock of plants. Currently, leaf movements are analysed using time lapse photography and subsequent bioinformatics analyses of leaf movements. Programs that are used for this purpose either are able to perform one function (i.e. leaf tip detection or rhythm analysis) or their function is limited to specific computational environments. We developed a leaf movement analysis tool—PALMA—that works in command line and combines image extraction with rhythm analysis using Fast Fourier transformation and non-linear least squares fitting. Results We validated PALMA in both simulated time series and in experiments using the known short period mutant sensitivity to red light reduced 1 (srr1-1). We compared PALMA with two established leaf movement analysis tools and found it to perform equally well. Finally, we tested the effect of reduced iron conditions on the leaf movement rhythms of wild type plants. Here, we found that PALMA successfully detected period lengthening under reduced iron conditions. Conclusions PALMA correctly estimated the period of both simulated and real-life leaf movement experiments. As a platform-independent console-program that unites both functions needed for the analysis of circadian leaf movements it is a valid alternative to existing leaf movement analysis tools