6,359 research outputs found
Detection of dirt impairments from archived film sequences : survey and evaluations
Film dirt is the most commonly encountered artifact in archive restoration applications. Since dirt usually appears as a temporally impulsive event, motion-compensated interframe processing is widely applied for its detection. However, motion-compensated prediction requires a high degree of complexity and can be unreliable when motion estimation fails. Consequently, many techniques using spatial or spatiotemporal filtering without motion were also been proposed as alternatives. A comprehensive survey and evaluation of existing methods is presented, in which both qualitative and quantitative performances are compared in terms of accuracy, robustness, and complexity. After analyzing these algorithms and identifying their limitations, we conclude with guidance in choosing from these algorithms and promising directions for future research
A multi-view approach to cDNA micro-array analysis
The official published version can be obtained from the link below.Microarray has emerged as a powerful technology that enables biologists to study thousands of genes simultaneously, therefore, to obtain a better understanding of the gene interaction and regulation mechanisms. This paper is concerned with improving the processes involved in the analysis of microarray image data. The main focus is to clarify an image's feature space in an unsupervised manner. In this paper, the Image Transformation Engine (ITE), combined with different filters, is investigated. The proposed methods are applied to a set of real-world cDNA images. The MatCNN toolbox is used during the segmentation process. Quantitative comparisons between different filters are carried out. It is shown that the CLD filter is the best one to be applied with the ITE.This work was supported in part by the Engineering and Physical Sciences Research
Council (EPSRC) of the UK under Grant GR/S27658/01, the National Science Foundation of China under Innovative Grant 70621001, Chinese Academy of Sciences
under Innovative Group Overseas Partnership Grant, the BHP Billiton Cooperation of Australia Grant, the International Science and Technology Cooperation Project of China
under Grant 2009DFA32050 and the Alexander von Humboldt Foundation of Germany
Segmentation-assisted detection of dirt impairments in archived film sequences
A novel segmentation-assisted method for film dirt detection is proposed. We exploit the fact that film dirt manifests in the spatial domain as a cluster of connected pixels whose intensity differs substantially from that of its neighborhood and we employ a segmentation-based approach to identify this type of structure. A key feature of our approach is the computation of a measure of confidence attached to detected dirt regions which can be utilized for performance fine tuning. Another important feature of our algorithm is the avoidance of the computational complexity associated with motion estimation. Our experimental framework benefits from the availability of manually derived as well as objective ground truth data obtained using infrared scanning. Our results demonstrate that the proposed method compares favorably with standard spatial, temporal and multistage median filtering approaches and provides efficient and robust detection for a wide variety of test material
Traction force microscopy with optimized regularization and automated Bayesian parameter selection for comparing cells
Adherent cells exert traction forces on to their environment, which allows
them to migrate, to maintain tissue integrity, and to form complex
multicellular structures. This traction can be measured in a perturbation-free
manner with traction force microscopy (TFM). In TFM, traction is usually
calculated via the solution of a linear system, which is complicated by
undersampled input data, acquisition noise, and large condition numbers for
some methods. Therefore, standard TFM algorithms either employ data filtering
or regularization. However, these approaches require a manual selection of
filter- or regularization parameters and consequently exhibit a substantial
degree of subjectiveness. This shortcoming is particularly serious when cells
in different conditions are to be compared because optimal noise suppression
needs to be adapted for every situation, which invariably results in systematic
errors. Here, we systematically test the performance of new methods from
computer vision and Bayesian inference for solving the inverse problem in TFM.
We compare two classical schemes, L1- and L2-regularization, with three
previously untested schemes, namely Elastic Net regularization, Proximal
Gradient Lasso, and Proximal Gradient Elastic Net. Overall, we find that
Elastic Net regularization, which combines L1 and L2 regularization,
outperforms all other methods with regard to accuracy of traction
reconstruction. Next, we develop two methods, Bayesian L2 regularization and
Advanced Bayesian L2 regularization, for automatic, optimal L2 regularization.
Using artificial data and experimental data, we show that these methods enable
robust reconstruction of traction without requiring a difficult selection of
regularization parameters specifically for each data set. Thus, Bayesian
methods can mitigate the considerable uncertainty inherent in comparing
cellular traction forces
Estimating Signals with Finite Rate of Innovation from Noisy Samples: A Stochastic Algorithm
As an example of the recently-introduced concept of rate of innovation,
signals that are linear combinations of a finite number of Diracs per unit time
can be acquired by linear filtering followed by uniform sampling. However, in
reality, samples are rarely noiseless. In this paper, we introduce a novel
stochastic algorithm to reconstruct a signal with finite rate of innovation
from its noisy samples. Even though variants of this problem has been
approached previously, satisfactory solutions are only available for certain
classes of sampling kernels, for example kernels which satisfy the Strang-Fix
condition. In this paper, we consider the infinite-support Gaussian kernel,
which does not satisfy the Strang-Fix condition. Other classes of kernels can
be employed. Our algorithm is based on Gibbs sampling, a Markov chain Monte
Carlo (MCMC) method. Extensive numerical simulations demonstrate the accuracy
and robustness of our algorithm.Comment: Submitted to IEEE Transactions on Signal Processin
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