Draft Genome Sequence of Two Strains of Xanthomonas arboricola Isolated from Prunus persica Which Are Dissimilar to Strains That Cause Bacterial Spot Disease on Prunus spp.

The draft genome sequences of two strains of Xanthomonas arboricola, isolated from asymptomatic peach trees in Spain, are reported here. These strains are avirulent and do not belong to the same phylogroup as X. arboricola pv. pruni, a causal agent of bacterial spot disease of stone fruits and almonds.This work was supported financially by the Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) project RTA2014- 00018-CO2-01.Publishe

Genome Sequence of the Acidophilic Sulfate-Reducing Peptococcaceae Strain CEB3

We report the draft genome of the Peptococcaceae strain CEB3 that originated from an acidic (pH 2.5) stream draining an abandoned copper mine. Strain CEB3 is one of the very few reported acidophilic sulfate-reducing isolates. The 5.04-Mb draft genome harbors 5,069 predicted protein-encoding and 66 RNA genes

Genome Sequence of the Moderately Acidophilic Sulfate-Reducing Firmicute Desulfosporosinus acididurans (Strain M1T)

Microbial dissimilatory sulfate reduction is commonplace in many anaerobic environments, though few acidophilic bacteria are known to mediate this process. We report the 4.64-Mb draft genome of the type strain of the moderate acidophile Desulfosporosinus acididurans, which was isolated from acidic sediment in a river draining the Soufrière volcano, Montserrat

Growth and gas-phase dependent transcription analysis of fermentation pathway in Caloramator celer

Caloramator celer strain JW/YL-NZ35, formerly known as Thermobrachium celere is a unique alkalithermophilic organism that has the potential to solve energy problems in the future because of its hydrogen production mechanism. However in order to use this organism in industrial scale, more intrinsic understanding of the genetic and metabolic functioning of the organism is needed. The aim of this work is to undertake a transcriptional analysis of the growth and gas phase dependant fermentation pathway in this organism. This is done in order to better understand the genetic factors involved in hydrogen production and to monitor the pattern of gene expression in different physiological states of the cell. This transcription analysis was done using a Real time quantitative PCR that uses a fluorescence molecule for real time detection. This is a very sensitive process that gives reliable results. Two different housekeeping genes recA and polC were used as normalizing factors in order to calculate the relative gene expression and 2-ΔΔCT method was used for this purpose. Through this analysis, the interdependence of formate, acetate and ethanol pathways with each other, and their combined effects on hydrogen production was studied. It was seen that formate and ethanol pathway is in direct competition with both the hydrogenases. Downregulation of hydrogenase genes because of ethanol and formate pathways was seen. It was also found that the cultures grown in artificially stressed gas phase media had a widespread downregultaion of its genes. The soluble metabolite was measured and it was correlated with the transcription data. Decrease of pH and increase of hydrogen partial pressure were the leading cause for the downregulation of mbhL and hydA hydrogenase genes. Increase in hydrogen partial pressure also led to increased ethanol production and adhE gene upregulation