Ethnobotany Study of Seaweed Diversity and Its Utilization in Warambadi, Panguhalodo Areas of East Sumba District

This paper reports the ethnobotany study of seaweed diversity in Warambadi –Panguhalodo areas of East Sumba District, the island of Sumba. The study recorded19 genera of 54 species of seaweed, which were utilized as food or edible seaweed.The group consisted of 17 species of green algae, 17 species of red algae, and 20species of brown algae. The study also reported that 18 genera of 38 species weretraditionally utilized for medicinal purposes as herbal medicine. The herbal speciesconsisted of 7 species of green algae, 13 species of red algae, and 18 species ofbrown algae.Seaweed is traditionally consumed as food in various forms: raw as salad and vegetable,as pickle with sauce of allspice or with vinegar, as relish or sweetened jellies and alsocooked for vegetable soup. As herbal medicine seaweed is usually used for traditionalcosmetics, as antipyretic and antiseptic, as vermifuges, and treatments for cough andasthma, hemorrhoid, nosebleed and boils, goiter and scrofula, stomach ailments andurinary diseases.Indigenous knowledge on seaweed still exist and are continually employed bypeople living in particular areas such as the Sumba and Sabu ethnic groups. Yet,the knowledge is gradually decreasing due to localities, socio-economic change andcultural development

Diversity of Antibacterial Compounds From Eucheuma Serra, Halimeda Opuntia, and Hydroclathrus Clathratus

Fourteen compounds were isolated from acetone extracts of three species of seaweeds (Eucheuma serra, a red seaweed, Halimeda opuntia, a green seaweed, and Hydroclathrus clathratus, a brown seaweed) using bioautographic TLC methods and identified using GC-MS. From Eucheuma serra were isolated 8 compounds (3 fatty acids, 3 steroids, and 2 aldehyds). Only two compounds of fatty acid camefrom Halimeda opuntia, whereas Hydroclathrus clathratus produced 6 compounds (4 fatty acids, one compound each of steroid and ether). All isolated single compoundswere tested for their antibacterial activities by the agar diffusion method against the Gram-positive bacteria Staphylococcus aureus, Bacillus subtilis and Streptococcusfaecalis, and the Gram-negative bacteria Echerichia coli, Pseudomonas aeruginosa and Salmonella typhimurium. All 14 compounds showed activity against Gram-positivebacteria, especially Bacillus subtilis, and only 2 compounds showed activity against Gram-negative bacteria Escherichia coli. Nine compounds showed activity against Staphylococcus aureus, and 4 compounds showed activity against Streptococcusfaecalis. All compounds were not active against Pseudomonas aeruginosa andSalmonella typhimurium bacteria. This study indicated that there is indeed a diversityboth in kinds and in molecular structures of the antibacterial substances

ANTIOXIDANT ACTIVITY AND TOXICITY POLYSACCHARIDE EXTRACT FROM RED ALGAE Eucheuma cottonii AND Eucheuma spinosum

Red algae Eucheuma cottonii and Eucheuma spinosum which one alternative source of antioxidant and anticancer because contains polysaccharide compound. This research aims to isolate and examine the activity of antioxidant and toxicity polysaccharide extract from red algae Eucheuma cottonii and Eucheuma spinosum . The study was carried out by isolating the polysaccharide extract using water and methanol-ethanol precipitation. Antioxidant activity of crude extract was examined using 1,1-Difenil-2-pikrilhidrazil (DPPH) method, and toxicity test was carried out using Brine Shrimp Lethality Test (BSLT). The research results showed that the crude extract polysaccharide for Eucheuma cottonii and Eucheuma spinosum have strong antioxidant activity with IC50 value of 72,49 ppm and 75,98 ppm. The result of BSLT assay showed that the crude extract polysaccharide has a highest toxicity with LC50 value of 165,88 ppm and 337,21 ppm there are classified as toxic. The crude extract polysaccharide Eucheuma cottonii and Eucheuma spinosum has a potential to be developed as an alternative antioxidant and anticancer agent

Utilization of seaweed resources

A brief discussion is presented on the commercial importance of seaweeds in the Philippines, which is mainly concerned with their use as sources of industrial gums such as agar, carrageenan, and alginic acid. Carrageenan as a substitute for microbiological agar and the use of seaweeds as a binder of heavy metal pollutants are examined