Problems of Corneal Endothelial Image Binarization

In this paper we present two methods of binarization of corneal endothelial images. The binarization is a first step of advanced image analysis. Images of corneal endothelial obtained by the specular microscopy have a poor dynamic range and they are usually non-uniformly illuminated. The binarization endothelial images is not trivial. Two binarization algorithms are proposed. The output images are presented. The quality of algorithms is discussed

Sensing and Signal Processing in Smart Healthcare

In the last decade, we have witnessed the rapid development of electronic technologies that are transforming our daily lives. Such technologies are often integrated with various sensors that facilitate the collection of human motion and physiological data and are equipped with wireless communication modules such as Bluetooth, radio frequency identification, and near-field communication. In smart healthcare applications, designing ergonomic and intuitive human–computer interfaces is crucial because a system that is not easy to use will create a huge obstacle to adoption and may significantly reduce the efficacy of the solution. Signal and data processing is another important consideration in smart healthcare applications because it must ensure high accuracy with a high level of confidence in order for the applications to be useful for clinicians in making diagnosis and treatment decisions. This Special Issue is a collection of 10 articles selected from a total of 26 contributions. These contributions span the areas of signal processing and smart healthcare systems mostly contributed by authors from Europe, including Italy, Spain, France, Portugal, Romania, Sweden, and Netherlands. Authors from China, Korea, Taiwan, Indonesia, and Ecuador are also included

Anatomical Modeling of Cerebral Microvascular Structures: Application to Identify Biomarkers of Microstrokes

Les réseaux microvasculaires corticaux sont responsables du transport de l’oxygène et des substrats énergétiques vers les neurones. Ces réseaux réagissent dynamiquement aux demandes énergétiques lors d’une activation neuronale par le biais du couplage neurovasculaire. Afin d’élucider le rôle de la composante microvasculaire dans ce processus de couplage, l’utilisation de la modélisation in-formatique pourrait se révéler un élément clé. Cependant, la manque de méthodologies de calcul appropriées et entièrement automatisées pour modéliser et caractériser les réseaux microvasculaires reste l’un des principaux obstacles. Le développement d’une solution entièrement automatisée est donc important pour des explorations plus avancées, notamment pour quantifier l’impact des mal-formations vasculaires associées à de nombreuses maladies cérébrovasculaires. Une observation courante dans l’ensemble des troubles neurovasculaires est la formation de micro-blocages vascu-laires cérébraux (mAVC) dans les artérioles pénétrantes de la surface piale. De récents travaux ont démontré l’impact de ces événements microscopiques sur la fonction cérébrale. Par conséquent, il est d’une importance vitale de développer une approche non invasive et comparative pour identifier leur présence dans un cadre clinique. Dans cette thèse,un pipeline de traitement entièrement automatisé est proposé pour aborder le prob-lème de la modélisation anatomique microvasculaire. La méthode de modélisation consiste en un réseau de neurones entièrement convolutif pour segmenter les capillaires sanguins, un générateur de modèle de surface 3D et un algorithme de contraction de la géométrie pour produire des mod-èles graphiques vasculaires ne comportant pas de connections multiples. Une amélioration de ce pipeline est développée plus tard pour alléger l’exigence de maillage lors de la phase de représen-tation graphique. Un nouveau schéma permettant de générer un modèle de graphe est développé avec des exigences d’entrée assouplies et permettant de retenir les informations sur les rayons des vaisseaux. Il est inspiré de graphes géométriques déformants construits en respectant les morpholo-gies vasculaires au lieu de maillages de surface. Un mécanisme pour supprimer la structure initiale du graphe à chaque exécution est implémenté avec un critère de convergence pour arrêter le pro-cessus. Une phase de raffinement est introduite pour obtenir des modèles vasculaires finaux. La modélisation informatique développée est ensuite appliquée pour simuler les signatures IRM po-tentielles de mAVC, combinant le marquage de spin artériel (ASL) et l’imagerie multidirectionnelle pondérée en diffusion (DWI). L’hypothèse est basée sur des observations récentes démontrant une réorientation radiale de la microvascularisation dans la périphérie du mAVC lors de la récupéra-tion chez la souris. Des lits capillaires synthétiques, orientés aléatoirement et radialement, et des angiogrammes de tomographie par cohérence optique (OCT), acquis dans le cortex de souris (n = 5) avant et après l’induction d’une photothrombose ciblée, sont analysés. Les graphes vasculaires informatiques sont exploités dans un simulateur 3D Monte-Carlo pour caractériser la réponse par résonance magnétique (MR), tout en considérant les effets des perturbations du champ magnétique causées par la désoxyhémoglobine, et l’advection et la diffusion des spins nucléaires. Le pipeline graphique proposé est validé sur des angiographies synthétiques et réelles acquises avec différentes modalités d’imagerie. Comparé à d’autres méthodes effectuées dans le milieu de la recherche, les expériences indiquent que le schéma proposé produit des taux d’erreur géométriques et topologiques amoindris sur divers angiogrammes. L’évaluation confirme également l’efficacité de la méthode proposée en fournissant des modèles représentatifs qui capturent tous les aspects anatomiques des structures vasculaires. Ensuite, afin de trouver des signatures de mAVC basées sur le signal IRM, la modélisation vasculaire proposée est exploitée pour quantifier le rapport de perte de signal intravoxel minimal lors de l’application de plusieurs directions de gradient, à des paramètres de séquence variables avec et sans ASL. Avec l’ASL, les résultats démontrent une dif-férence significative (p <0,05) entre le signal calculé avant et 3 semaines après la photothrombose. La puissance statistique a encore augmenté (p <0,005) en utilisant des angiogrammes capturés à la semaine suivante. Sans ASL, aucun changement de signal significatif n’est trouvé. Des rapports plus élevés sont obtenus à des intensités de champ magnétique plus faibles (par exemple, B0 = 3) et une lecture TE plus courte (<16 ms). Cette étude suggère que les mAVC pourraient être carac-térisés par des séquences ASL-DWI, et fournirait les informations nécessaires pour les validations expérimentales postérieures et les futurs essais comparatifs.----------ABSTRACT Cortical microvascular networks are responsible for carrying the necessary oxygen and energy substrates to our neurons. These networks react to the dynamic energy demands during neuronal activation through the process of neurovascular coupling. A key element in elucidating the role of the microvascular component in the brain is through computational modeling. However, the lack of fully-automated computational frameworks to model and characterize these microvascular net-works remains one of the main obstacles. Developing a fully-automated solution is thus substantial for further explorations, especially to quantify the impact of cerebrovascular malformations associ-ated with many cerebrovascular diseases. A common pathogenic outcome in a set of neurovascular disorders is the formation of microstrokes, i.e., micro occlusions in penetrating arterioles descend-ing from the pial surface. Recent experiments have demonstrated the impact of these microscopic events on brain function. Hence, it is of vital importance to develop a non-invasive and translatable approach to identify their presence in a clinical setting. In this thesis, a fully automatic processing pipeline to address the problem of microvascular anatom-ical modeling is proposed. The modeling scheme consists of a fully-convolutional neural network to segment microvessels, a 3D surface model generator and a geometry contraction algorithm to produce vascular graphical models with a single connected component. An improvement on this pipeline is developed later to alleviate the requirement of water-tight surface meshes as inputs to the graphing phase. The novel graphing scheme works with relaxed input requirements and intrin-sically captures vessel radii information, based on deforming geometric graphs constructed within vascular boundaries instead of surface meshes. A mechanism to decimate the initial graph struc-ture at each run is formulated with a convergence criterion to stop the process. A refinement phase is introduced to obtain final vascular models. The developed computational modeling is then ap-plied to simulate potential MRI signatures of microstrokes, combining arterial spin labeling (ASL) and multi-directional diffusion-weighted imaging (DWI). The hypothesis is driven based on recent observations demonstrating a radial reorientation of microvasculature around the micro-infarction locus during recovery in mice. Synthetic capillary beds, randomly- and radially oriented, and op-tical coherence tomography (OCT) angiograms, acquired in the barrel cortex of mice (n=5) before and after inducing targeted photothrombosis, are analyzed. The computational vascular graphs are exploited within a 3D Monte-Carlo simulator to characterize the magnetic resonance (MR) re-sponse, encompassing the effects of magnetic field perturbations caused by deoxyhemoglobin, and the advection and diffusion of the nuclear spins. The proposed graphing pipeline is validated on both synthetic and real angiograms acquired with different imaging modalities. Compared to other efficient and state-of-the-art graphing schemes, the experiments indicate that the proposed scheme produces the lowest geometric and topological error rates on various angiograms. The evaluation also confirms the efficiency of the proposed scheme in providing representative models that capture all anatomical aspects of vascular struc-tures. Next, searching for MRI-based signatures of microstokes, the proposed vascular modeling is exploited to quantify the minimal intravoxel signal loss ratio when applying multiple gradient di-rections, at varying sequence parameters with and without ASL. With ASL, the results demonstrate a significant difference (p<0.05) between the signal-ratios computed at baseline and 3 weeks after photothrombosis. The statistical power further increased (p<0.005) using angiograms captured at week 4. Without ASL, no reliable signal change is found. Higher ratios with improved significance are achieved at low magnetic field strengths (e.g., at 3 Tesla) and shorter readout TE (<16 ms). This study suggests that microstrokes might be characterized through ASL-DWI sequences, and provides necessary insights for posterior experimental validations, and ultimately, future transla-tional trials

Multi-resolution Active Models for Image Segmentation

Image segmentation refers to the process of subdividing an image into a set of non-overlapping regions. Image segmentation is a critical and essential step to almost all higher level image processing and pattern recognition approaches, where a good segmentation relieves higher level applications from considering irrelevant and noise data in the image. Image segmentation is also considered as the most challenging image processing step due to several reasons including spatial discontinuity of the region of interest and the absence of a universally accepted criteria for image segmentation. Among the huge number of segmentation approaches, active contour models or simply snakes receive a great attention in the literature. Where the contour/boundary of the region of interest is defined as the set of pixels at which the active contour reaches its equilibrium state. In general, two forces control the movement of the snake inside the image, internal force that prevents the snake from stretching and bending and external force that pulls the snake towards the desired object boundaries. One main limitation of active contour models is their sensitivity to image noise. Specifically, noise sensitivity leads the active contour to fail to properly converge, getting caught on spurious image features, preventing the iterative solver from taking large steps towards the final contour. Additionally, active contour initialization forms another type of limitation. Where, especially in noisy images, the active contour needs to be initialized relatively close to the object of interest, otherwise the active contour will be pulled by other non-real/spurious image features. This dissertation, aiming to improve the active model-based segmentation, introduces two models for building up the external force of the active contour. The first model builds up a scale-based-weighted gradient map from all resolutions of the undecimated wavelet transform, with preference given to coarse gradients over fine gradients. The undecimated wavelet transform, due to its near shift-invariance and the absence of down-sampling properties, produces well-localized gradient maps at all resolutions of the transform. Hence, the proposed final weighted gradient map is able to better drive the snake towards its final equilibrium state. Unlike other multiscale active contour algorithms that define a snake at each level of the hierarchy, our model defines a single snake with the external force field is simultaneously built based on gradient maps from all scales. The second model proposes the incorporation of the directional information, revealed by the dual tree complex wavelet transform (DT CWT), into the external force field of the active contour. At each resolution of the transform, a steerable set of convolution kernels is created and used for external force generation. In the proposed model, the size and the orientation of the kernels depend on the scale of the DT CWT and the local orientation statistics of each pixel. Experimental results using nature, synthetic and Optical Coherent Tomography (OCT) images reflect the superiority of the proposed models over the classical and the state-of-the-art models

Distortion-constraint compression of three-dimensional CLSM images using image pyramid and vector quantization

The confocal microscopy imaging techniques, which allow optical sectioning, have been successfully exploited in biomedical studies. Biomedical scientists can benefit from more realistic visualization and much more accurate diagnosis by processing and analysing on a three-dimensional image data. The lack of efficient image compression standards makes such large volumetric image data slow to transfer over limited bandwidth networks. It also imposes large storage space requirements and high cost in archiving and maintenance. Conventional two-dimensional image coders do not take into account inter-frame correlations in three-dimensional image data. The standard multi-frame coders, like video coders, although they have good performance in capturing motion information, are not efficiently designed for coding multiple frames representing a stack of optical planes of a real object. Therefore a real three-dimensional image compression approach should be investigated. Moreover the reconstructed image quality is a very important concern in compressing medical images, because it could be directly related to the diagnosis accuracy. Most of the state-of-the-arts methods are based on transform coding, for instance JPEG is based on discrete-cosine-transform CDCT) and JPEG2000 is based on discrete- wavelet-transform (DWT). However in DCT and DWT methods, the control of the reconstructed image quality is inconvenient, involving considerable costs in computation, since they are fundamentally rate-parameterized methods rather than distortion-parameterized methods. Therefore it is very desirable to develop a transform-based distortion-parameterized compression method, which is expected to have high coding performance and also able to conveniently and accurately control the final distortion according to the user specified quality requirement. This thesis describes our work in developing a distortion-constraint three-dimensional image compression approach, using vector quantization techniques combined with image pyramid structures. We are expecting our method to have: 1. High coding performance in compressing three-dimensional microscopic image data, compared to the state-of-the-art three-dimensional image coders and other standardized two-dimensional image coders and video coders. 2. Distortion-control capability, which is a very desirable feature in medical 2. Distortion-control capability, which is a very desirable feature in medical image compression applications, is superior to the rate-parameterized methods in achieving a user specified quality requirement. The result is a three-dimensional image compression method, which has outstanding compression performance, measured objectively, for volumetric microscopic images. The distortion-constraint feature, by which users can expect to achieve a target image quality rather than the compressed file size, offers more flexible control of the reconstructed image quality than its rate-constraint counterparts in medical image applications. Additionally, it effectively reduces the artifacts presented in other approaches at low bit rates and also attenuates noise in the pre-compressed images. Furthermore, its advantages in progressive transmission and fast decoding make it suitable for bandwidth limited tele-communications and web-based image browsing applications

Mammography

In this volume, the topics are constructed from a variety of contents: the bases of mammography systems, optimization of screening mammography with reference to evidence-based research, new technologies of image acquisition and its surrounding systems, and case reports with reference to up-to-date multimodality images of breast cancer. Mammography has been lagged in the transition to digital imaging systems because of the necessity of high resolution for diagnosis. However, in the past ten years, technical improvement has resolved the difficulties and boosted new diagnostic systems. We hope that the reader will learn the essentials of mammography and will be forward-looking for the new technologies. We want to express our sincere gratitude and appreciation?to all the co-authors who have contributed their work to this volume

Brain and Human Body Modeling

This open access book describes modern applications of computational human modeling with specific emphasis in the areas of neurology and neuroelectromagnetics, depression and cancer treatments, radio-frequency studies and wireless communications. Special consideration is also given to the use of human modeling to the computational assessment of relevant regulatory and safety requirements. Readers working on applications that may expose human subjects to electromagnetic radiation will benefit from this book’s coverage of the latest developments in computational modelling and human phantom development to assess a given technology’s safety and efficacy in a timely manner. Describes construction and application of computational human models including anatomically detailed and subject specific models; Explains new practices in computational human modeling for neuroelectromagnetics, electromagnetic safety, and exposure evaluations; Includes a survey of modern applications for which computational human models are critical; Describes cellular-level interactions between the human body and electromagnetic fields

Brain and Human Body Modeling

This open access book describes modern applications of computational human modeling with specific emphasis in the areas of neurology and neuroelectromagnetics, depression and cancer treatments, radio-frequency studies and wireless communications. Special consideration is also given to the use of human modeling to the computational assessment of relevant regulatory and safety requirements. Readers working on applications that may expose human subjects to electromagnetic radiation will benefit from this book’s coverage of the latest developments in computational modelling and human phantom development to assess a given technology’s safety and efficacy in a timely manner. Describes construction and application of computational human models including anatomically detailed and subject specific models; Explains new practices in computational human modeling for neuroelectromagnetics, electromagnetic safety, and exposure evaluations; Includes a survey of modern applications for which computational human models are critical; Describes cellular-level interactions between the human body and electromagnetic fields

Image Restoration

This book represents a sample of recent contributions of researchers all around the world in the field of image restoration. The book consists of 15 chapters organized in three main sections (Theory, Applications, Interdisciplinarity). Topics cover some different aspects of the theory of image restoration, but this book is also an occasion to highlight some new topics of research related to the emergence of some original imaging devices. From this arise some real challenging problems related to image reconstruction/restoration that open the way to some new fundamental scientific questions closely related with the world we interact with