9 research outputs found
Endoscopic Optical Coherence Tomography: Design and Application
This thesis presents an investigation on endoscopic optical coherence tomography (OCT). As a noninvasive imaging modality, OCT emerges as an increasingly important diagnostic tool for many clinical applications. Despite of many of its merits, such as high resolution and depth resolvability, a major limitation is the relatively shallow penetration depth in tissue (about 2∼3 mm). This is mainly due to tissue scattering and absorption. To overcome this limitation, people have been developing many different endoscopic OCT systems. By utilizing a minimally invasive endoscope, the OCT probing beam can be brought to the close vicinity of the tissue of interest and bypass the scattering of intervening tissues so that it can collect the reflected light signal from desired depth and provide a clear image representing the physiological structure of the region, which can not be disclosed by traditional OCT. In this thesis, three endoscope designs have been studied. While they rely on vastly different principles, they all converge to solve this long-standing problem.
A hand-held endoscope with manual scanning is first explored. When a user is holding a hand- held endoscope to examine samples, the movement of the device provides a natural scanning. We proposed and implemented an optical tracking system to estimate and record the trajectory of the device. By registering the OCT axial scan with the spatial information obtained from the tracking system, one can use this system to simply ‘paint’ a desired volume and get any arbitrary scanning pattern by manually waving the endoscope over the region of interest. The accuracy of the tracking system was measured to be about 10 microns, which is comparable to the lateral resolution of most OCT system. Targeted phantom sample and biological samples were manually scanned and the reconstructed images verified the method.
Next, we investigated a mechanical way to steer the beam in an OCT endoscope, which is termed as Paired-angle-rotation scanning (PARS). This concept was proposed by my colleague and we further developed this technology by enhancing the longevity of the device, reducing the diameter of the probe, and shrinking down the form factor of the hand-piece. Several families of probes have been designed and fabricated with various optical performances. They have been applied to different applications, including the collector channel examination for glaucoma stent implantation, and vitreous remnant detection during live animal vitrectomy.
Lastly a novel non-moving scanning method has been devised. This approach is based on the EO effect of a KTN crystal. With Ohmic contact of the electrodes, the KTN crystal can exhibit a special mode of EO effect, termed as space-charge-controlled electro-optic effect, where the carrier electron will be injected into the material via the Ohmic contact. By applying a high voltage across the material, a linear phase profile can be built under this mode, which in turn deflects the light beam passing through. We constructed a relay telescope to adapt the KTN deflector into a bench top OCT scanning system. One of major technical challenges for this system is the strong chromatic dispersion of KTN crystal within the wavelength band of OCT system. We investigated its impact on the acquired OCT images and proposed a new approach to estimate and compensate the actual dispersion. Comparing with traditional methods, the new method is more computational efficient and accurate. Some biological samples were scanned by this KTN based system. The acquired images justified the feasibility of the usage of this system into a endoscopy setting.
My research above all aims to provide solutions to implement an OCT endoscope. As technology evolves from manual, to mechanical, and to electrical approaches, different solutions are presented. Since all have their own advantages and disadvantages, one has to determine the actual requirements and select the best fit for a specific application.</p
828 kHz retinal imaging with an 840 nm Fourier domain mode locked laser
This paper presents a Fourier domain mode locked (FDML) laser centered around 840 nm. It features a bidirectional sweep repetition rate of 828 kHz and a spectral bandwidth of 40 nm. An axial resolution of ∼9.9 μm in water and a 1.4 cm sensitivity roll-off are achieved. Utilizing a complex master-slave (CMS) recalibration method and due to a sufficiently high sensitivity of 84.6 dB, retinal layers of the human eye in-vivo can be resolved during optical coherence tomography (OCT) examination. The developed FDML laser enables acquisition rates of 3D-volumes with a size of 200 × 100 × 256 voxels in under 100 milliseconds. Detailed information on the FDML implementation, its challenging design tasks, and OCT images obtained with the laser are presented in this paper
Dual beam swept source optical coherence tomography for microfluidic velocity measurements
Microfluidic flows are an increasing area of interest used for “lab-on-a-chip” bioanalytical
techniques, drug discovery, and chemical processing. This requires optical,
non-invasive flow-visualization techniques for characterising microfluidic flows. Optical
Coherence Tomography (OCT) systems can provide three-dimensional imaging
through reasonably-opaque materials with micrometre resolution, coupled to a single
optical axis point using optical fibre cables. Developed for imaging the human eye,
OCT has been used for the detection of skin cancers and endoscopically in the human
body. Industrial applications are growing in popularity including for the monitoring
of bond-curing in aerospace, for production-line non-destructive-testing, and for
medical device manufacturing and drug encapsulation monitoring.
A dual beam Optical Coherence Tomography system has been developed capable
of simultaneously imaging microfluidic channel structures, and tracking particles
seeded into the flow to measure high velocity flows, using only a single optical access
point. This is achieved via a dual optical fibre bundle for light delivery to the sample
and a custom high-speed dual channel OCT instrument using an akinetic sweep
wavelength laser. The system has 10 μm resolution in air and a sweeping rate of
96 kHz. This OCT system was used to monitor microfluidic flows in 800 μm deep
test chips and Poiseuille flows were observed
Parametric analysis of microwave and laser systems for communication and tracking Quarterly report, 6 Mar. - 6 Jun. 1966
Parametric analysis of microwave and laser systems for communication and tracking - updated reference data for advanced space communication and tracking system
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High-speed phase-stable swept source optical coherence tomography: functional imaging and biomedical applications
In the past decades, the performance of swept source optical coherence tomography (SS-OCT) has experienced an unprecedented improvement which is mainly driven by the rapidly evolving laser technologies: the state-of-art SS-OCT is now tens of dB more sensitive, six orders of magnitude faster, and seeing ten times deeper than the original version of time domain OCT. Regardless of the abovementioned progress, the phase instability is always considered the biggest weakness of SS-OCT and the mainstream belief often states that the mechanical tuning mechanism of the swept source is to blame.
In my study, I first developed a high-speed phase-stable SS-OCT based on a new-generation akinetic laser source, which is electrically tuned in wavelength, in the hope of reducing the phase noise to a shot-noise limited level. The experimental results turned out to be contradicted to the conventional phase noise theory, which inspires my discovery of a completely new interpretation for the phase noise in SS-OCT: I proposed that the timing jitter and scanning variability has to be taken into the consideration in the noise model as multiplicative noises. The theory was later validated by another SS-OCT using a different light source. This study for the first time articulated the phase noise’s origin and composition in the SS-OCT.
Although the SS-OCT performs relatively worse in phase stability compared with its spectral-domain counterpart (SD-OCT), it is still valuable since it images at a much faster rate than SD-OCT. Therefore, a better temporal resolution could be achieved, which is particularly attractive in areas such as time lapse imaging. I therefore utilize the system along with other two systems to conduct ex vivo imaging on human tracheobronchial epithelium. It is shown that the SS-OCT system could achieve equally good performance in this task. Moreover, thanks to the higher temporal and temporal frequency resolution, finer structure within the frequency response of the ciliary motion is picked up by our system.
During the study of ex vivo ciliary imaging, one of the challenges I was confronted with was the enormous amount of data generated by the SS-OCT, especially when high temporal frequency resolution is required. We thus came up with an idea of applying the compressive sensing (CS) to reduce the data size. Currently, we have demonstrated some preliminary results with using CS on reference k-clock channel compression. In the future, we will apply the same theory to compress the sample channel data, especially or time lapse OCT imaging
Semiannual progress report no. 1, 16 November 1964 - 30 June 1965
Summary reports of research in bioelectronics, electron streams and interactions, plasmas, quantum and optical electronics, radiation and propagation, and solid-state electronic