The effect of genome length on ejection forces in bacteriophage lambda

A variety of viruses tightly pack their genetic material into protein capsids that are barely large enough to enclose the genome. In particular, in bacteriophages, forces as high as 60 pN are encountered during packaging and ejection, produced by DNA bending elasticity and self-interactions. The high forces are believed to be important for the ejection process, though the extent of their involvement is not yet clear. As a result, there is a need for quantitative models and experiments that reveal the nature of the forces relevant to DNA ejection. Here we report measurements of the ejection forces for two different mutants of bacteriophage lambda, lambda b221cI26 and lambda cI60, which differ in genome length by ~30%. As expected for a force-driven ejection mechanism, the osmotic pressure at which DNA release is completely inhibited varies with the genome length: we find inhibition pressures of 15 atm and 25 atm, respectively, values that are in agreement with our theoretical calculations

Electronic and photonic switching in the atm era

Broadband networks require high-capacity switches in order to properly manage large amounts of traffic fluxes. Electronic and photonic technologies are being used to achieve this objective both allowing different multiplexing and switching techniques. Focusing on the asynchronous transfer mode (ATM), the inherent different characteristics of electronics and photonics makes different architectures feasible. In this paper, different switching structures are described, several ATM switching architectures which have been recently implemented are presented and the implementation characteristics discussed. Three diverse points of view are given from the electronic research, the photonic research and the commercial switches. Although all the architectures where successfully tested, they should also follow different market requirements in order to be commercialised. The characteristics are presented and the architectures projected over them to evaluate their commercial capabilities.Peer ReviewedPostprint (published version

Collective traffic-like movement of ants on a trail: dynamical phases and phase transitions

The traffic-like collective movement of ants on a trail can be described by a stochastic cellular automaton model. We have earlier investigated its unusual flow-density relation by using various mean field approximations and computer simulations. In this paper, we study the model following an alternative approach based on the analogy with the zero range process, which is one of the few known exactly solvable stochastic dynamical models. We show that our theory can quantitatively account for the unusual non-monotonic dependence of the average speed of the ants on their density for finite lattices with periodic boundary conditions. Moreover, we argue that the model exhibits a continuous phase transition at the critial density only in a limiting case. Furthermore, we investigate the phase diagram of the model by replacing the periodic boundary conditions by open boundary conditions.Comment: 8 pages, 6 figure

Multiplexing regulated traffic streams: design and performance

The main network solutions for supporting QoS rely on traf- fic policing (conditioning, shaping). In particular, for IP networks the IETF has developed Intserv (individual flows regulated) and Diffserv (only ag- gregates regulated). The regulator proposed could be based on the (dual) leaky-bucket mechanism. This explains the interest in network element per- formance (loss, delay) for leaky-bucket regulated traffic. This paper describes a novel approach to the above problem. Explicitly using the correlation structure of the sources’ traffic, we derive approxi- mations for both small and large buffers. Importantly, for small (large) buffers the short-term (long-term) correlations are dominant. The large buffer result decomposes the traffic stream in a stream of constant rate and a periodic impulse stream, allowing direct application of the Brownian bridge approximation. Combining the small and large buffer results by a concave majorization, we propose a simple, fast and accurate technique to statistically multiplex homogeneous regulated sources. To address heterogeneous inputs, we present similarly efficient tech- niques to evaluate the performance of multiple classes of traffic, each with distinct characteristics and QoS requirements. These techniques, applica- ble under more general conditions, are based on optimal resource (band- width and buffer) partitioning. They can also be directly applied to set GPS (Generalized Processor Sharing) weights and buffer thresholds in a shared resource system