Role of shear stress in endothelial cell morphology and expression of cyclooxygenase isoforms

MEDLINE® is the source for the MeSH terms of this document.Objective-: The goal of this study was to examine the effect of chronic heterogeneous shear stress, applied using an orbital shaker, on endothelial cell morphology and the expression of cyclooxygenases 1 and 2. Methods and results-: Porcine aortic endothelial cells were plated on fibronectin-coated Transwell plates. Cells were cultured for up to 7 days either under static conditions or on an orbital shaker that generated a wave of medium inducing shear stress over the cells. Cells were fixed and stained for the endothelial surface marker CD31 or cyclooxygenases 1 and 2. En face confocal microscopy and scanning ion conductance microscopy were used to show that endothelial cells were randomly oriented at the center of the well, aligned with shear stress nearer the periphery, and expressed cyclooxygenase-1 under all conditions. Lipopolysaccharide induced cyclooxygenase-2 and the production of 6-keto-prostaglandin F1α in all cells. Conclusion-: Cyclooxygenase-1 is expressed in endothelial cells cultured under chronic shear stress of high or low directionality.Peer reviewedSubmitted Versio

A comparison of multiprocessor scheduling methods for iterative data flow architectures

A comparative study is made between the Algorithm to Architecture Mapping Model (ATAMM) and three other related multiprocessing models from the published literature. The primary focus of all four models is the non-preemptive scheduling of large-grain iterative data flow graphs as required in real-time systems, control applications, signal processing, and pipelined computations. Important characteristics of the models such as injection control, dynamic assignment, multiple node instantiations, static optimum unfolding, range-chart guided scheduling, and mathematical optimization are identified. The models from the literature are compared with the ATAMM for performance, scheduling methods, memory requirements, and complexity of scheduling and design procedures

Applying Dataflow Analysis to Dimension Buffers for Guaranteed Performance in Networks on Chip

A Network on Chip (NoC) with end-to-end flow control is modelled by a cyclo-static dataflow graph. Using the proposed model together with state-of-the-art dataflow analysis algorithms, we size the buffers in the network interfaces. We show, for a range of NoC designs, that buffer sizes are determined with a run time comparable to existing analytical methods, and results comparable to exhaustive simulation

Doughnut strikes sandwich: the geometry of hot medium in accreting black hole X-ray binaries

We study the effects of the mutual interaction of hot plasma and cold medium in black hole binaries in their hard spectral state on the value of the truncation radii of accretion discs. We consider a number of different geometries. In contrast to previous theoretical studies, we use a modern energy-conserving code for reflection and reprocessing from cold media. We show that a static corona above a disc extending to the innermost stable circular orbit produces spectra not compatible with those observed. They are either too soft or require a much higher disc ionization than that observed. This conclusion confirms a number of previous findings, but disproves a recent study claiming an agreement of that model with observations. We show that the cold disc has to be truncated in order to agree with the observed spectral hardness. However, a cold disc truncated at a large radius and replaced by a hot flow produces spectra which are too hard if the only source of seed photons for Comptonization is the accretion disc. Our favourable geometry is a truncated disc coexisting with a hot plasma either overlapping with the disc or containing some cold matter within it, also including seed photons arising from cyclo-synchrotron emission of hybrid electrons, i.e. containing both thermal and non-thermal parts.Comment: 12 pages, 8 figures; A&A, in pres

Omphale: Streamlining the Communication for Jobs in a Multi Processor System on Chip

Our Multi Processor System on Chip (MPSoC) template provides processing tiles that are connected via a network on chip. A processing tile contains a processing unit and a Scratch Pad Memory (SPM). This paper presents the Omphale tool that performs the first step in mapping a job, represented by a task graph, to such an MPSoC, given the SPM sizes as constraints. Furthermore a memory tile is introduced. The result of Omphale is a Cyclo Static DataFlow (CSDF) model and a task graph where tasks communicate via sliding windows that are located in circular buffers. The CSDF model is used to determine the size of the buffers and the communication pattern of the data. A buffer must fit in the SPM of the processing unit that is reading from it, such that low latency access is realized with a minimized number of stall cycles. If a task and its buffer exceed the size of the SPM, the task is examined for additional parallelism or the circular buffer is partly located in a memory tile. This results in an extended task graph that satisfies the SPM size constraints

Magnetic flares and the optical variability of the X-ray transient XTE J1118+480

The simultaneous presence of a strong quasi periodic oscillation of period of about 10 seconds in the optical and X-ray lightcurves of the X-ray transient XTE J1118+480 suggests that a significant fraction of the optical flux originates from the inner part of the accretion flow, where most of the X-rays are produced. We present a model of magnetic flares in an accretion disc corona where thermal cyclo-synchrotron emission contributes significantly to the optical emission, while the X-rays are produced by inverse Compton scattering of the soft photons produced by dissipation in the underlying disc and by the synchrotron process itself. Given the observational constraints, we estimate the values for the coronal temperature, optical depth and magnetic field intensity, as well as the accretion rate for the source. Within our model we predict a correlation between optical and hard X-ray variability and an anticorrelation between optical and soft X-rays. We also expect optical variability on flaring timescales (about tens of milliseconds), with a power density spectrum similar to the one observed in the X-ray band. Finally we use both the available optical/EUV/X-ray spectral energy distribution and the low frequency time variability to discuss limits on the inner radius of the optically thick disc.Comment: 5 pages, included 1 figure. One reference corrected. Submitted to MNRA

Computation of Buffer Capacities for Throughput Constrained and Data Dependent Inter-Task Communication

Streaming applications are often implemented as task graphs. Currently, techniques exist to derive buffer capacities that guarantee satisfaction of a throughput constraint for task graphs in which the inter-task communication is data-independent, i.e. the amount of data produced and consumed is independent of the data values in the processed stream. This paper presents a technique to compute buffer capacities that satisfy a throughput constraint for task graphs with data dependent inter-task communication, given that the task graph is a chain. We demonstrate the applicability of the approach by computing buffer capacities for an MP3 playback application, of which the MP3 decoder has a variable consumption rate. We are not aware of alternative approaches to compute buffer capacities that guarantee satisfaction of the throughput constraint for this application

Modeling heterogeneous processor scheduling for real time systems

A new model is presented to describe dataflow algorithms implemented in a multiprocessing system. Called the resource/data flow graph (RDFG), the model explicitly represents cyclo-static processor schedules as circuits of processor arcs which reflect the order that processors execute graph nodes. The model also allows the guarantee of meeting hard real-time deadlines. When unfolded, the model identifies statically the processor schedule. The model therefore is useful for determining the throughput and latency of systems with heterogeneous processors. The applicability of the model is demonstrated using a space surveillance algorithm