Two-Dimensional Bunch-Resolved Optical Beam Diagnostics at BESSY II

BESSY II ist eine Strahlungsquelle der dritten Generation, die vom Helmholtz-Zentrum Berlin für Materialien und Energie GmbH für Experimente mit Synchrotronstrahlung betrieben wird. Mehrere Betriebsmodi werden am BESSY II Speicherring angeboten bzw. entwickelt, um die Anforderungen der vielfältigen Nutzergemeinde zu erfüllen. Dazu gehören nicht nur ein komplexes Füllmuster im Standardnutzerbetrieb, sondern auch spezielle Betriebsmodi mit kurzen Pulsen oder das sogenannte Transverse Resonant Island Buckets Separationsschema. Die Komplexität des Füllmusters erfordert eine pulsaufgelöste Strahldiagnose für die Inbetriebnahme und zur Sicherstellung der langfristigen Qualität des Beschleunigerbetriebs. Ferner werden für den Kurzpulsbetrieb Pulslängenmessungen mit ps Auflösung benötigt. Im Rahmen dieser Arbeit wird zu diesem Zweck eine neue Diagnoseplattform mit mehreren Strahlrohren aufgebaut. Jeweils ein Strahlrohr ist für transversale Strahlgrößenmessungen und für longitudinale Strahldiagnose vorgesehen. Beide Strahlrohre sind mit Messapperaturen für pulsaufgelöste Messungen ausgestattet. Hauptfokus dieser Arbeit liegen auf dem Design, der Installation und den Verbesserungen dieser Strahlrohre und den zugehörigen Meßgeräten in Kombination mit spezifischen Anwendungen in der Strahldiagnose an BESSY II. Im Allgemeinen erfordern Kopplungen zwischen Zeit- und Raumkoordinaten pulsselektive und korrelierte Detektionsmethoden mehrerer Parameter. Daher sind die longitudinale Diagnose sowie die Streak Kamera so optimiert worden, dass die direkte Abbildung des transversalen Strahlprofils möglich ist und sogar interferometrische Strahlgrößenmessungen durchführbar. Zusätzlich zur Zeitachse der Streak Kamera kann entweder die horizontale oder die vertikale Dimension des Strahls abgebildet werden und dadurch sind 2D-Messungen möglich. Mit dieser Methode wurden mehrere pulsaufgelöste 2D-Messungen durchgeführt und Analysemethoden entwickelt.BESSY II is a third generation light source operated by the Helmholtz-Zentrum Berlin für Materialien und Energie GmbH for experiments with synchrotron radiation. Multiple operation modes are offered or are under development at the BESSY II storage ring to serve the needs of its diverse user community. This does not only include a complex fill pattern in standard operation, but also special operation modes featuring short pulses or the new transverse resonant island buckets separation scheme. The complexity of the fill pattern requires bunch-resolved diagnostics for commissioning and to ensure the long-term quality of accelerator operation. In addition, short pulse operation demands bunch length measurements with ps resolution. For this purpose a new diagnostics platform featuring multiple beamlines is set up. One beamline is dedicated for transverse beam size measurements and one for longitudinal diagnostics. Both beamlines are equipped with fast gated devices for bunch-resolved measurements. Design, installation and improvements of these beamlines and the measuring devices are the main focus of this work, together with specific BESSY II bunch diagnostics applications. In general, coupling between time- and space-coordinates do call for bunch-selective and correlated multi-parameter detection methods. Thus, the longitudinal diagnostics beamline and the streak camera have been made capable of direct transverse beam-profile imaging and even interferometric beam size measurements are feasible. Either the horizontal or vertical beam dimension can be imaged in addition to the time axis of the streak camera and 2D measurements are possible. Taking advantage of these capabilities, multiple bunch-resolved 2D measurements have been performed and analysis methods have been developed

Investigation into the changing colonisation of skin bacteria during isotretinoin treatment for acne vulgaris

Poster presentatio

XII CIBIA Iberoamerican congress of food engineering: challenging food engineering as a driver towards sustainable food processing - Book of abstracts

The XII edition of CIBIA, Iberoamerican Congress of Food Engineering will take place, for the first time, in Portugal next July 1 to 4, having as theme “Challenging Food Engineering as a Driver Towards Sustainable Food Processing“. The host institution is the University of Algarve, Faro, in the Algarve region. The importance of the Food Engineering field was enhanced by the number of Iberoamerican researchers who participated in this XIIth edition of CIBIA. We received 530 abstracts submitted by researchers from the Iberian Peninsula (Portugal and Spain), and from all the South and Central American Continent: Brazil, Chile, Peru, Mexico, Colombia and Ecuador, among others. About 350 participants will be present with around 100 accepted abstracts for oral presentations and more than 300 poster presentations. These numbers reveal that Food Engineering is very alive and strong in the Iberoamerican world as many young researchers are pursuing their graduate studies in various disciplines of this field and senior researchers keep facing and studying new challenges such as the main Congress Theme “Challenging Food Engineering as a Driver towards Sustainable Food Processing”.info:eu-repo/semantics/publishedVersio

Ultrasensitive detection of toxocara canis excretory-secretory antigens by a nanobody electrochemical magnetosensor assay.

peer reviewedHuman Toxocariasis (HT) is a zoonotic disease caused by the migration of the larval stage of the roundworm Toxocara canis in the human host. Despite of being the most cosmopolitan helminthiasis worldwide, its diagnosis is elusive. Currently, the detection of specific immunoglobulins IgG against the Toxocara Excretory-Secretory Antigens (TES), combined with clinical and epidemiological criteria is the only strategy to diagnose HT. Cross-reactivity with other parasites and the inability to distinguish between past and active infections are the main limitations of this approach. Here, we present a sensitive and specific novel strategy to detect and quantify TES, aiming to identify active cases of HT. High specificity is achieved by making use of nanobodies (Nbs), recombinant single variable domain antibodies obtained from camelids, that due to their small molecular size (15kDa) can recognize hidden epitopes not accessible to conventional antibodies. High sensitivity is attained by the design of an electrochemical magnetosensor with an amperometric readout with all components of the assay mixed in one single step. Through this strategy, 10-fold higher sensitivity than a conventional sandwich ELISA was achieved. The assay reached a limit of detection of 2 and15 pg/ml in PBST20 0.05% or serum, spiked with TES, respectively. These limits of detection are sufficient to detect clinically relevant toxocaral infections. Furthermore, our nanobodies showed no cross-reactivity with antigens from Ascaris lumbricoides or Ascaris suum. This is to our knowledge, the most sensitive method to detect and quantify TES so far, and has great potential to significantly improve diagnosis of HT. Moreover, the characteristics of our electrochemical assay are promising for the development of point of care diagnostic systems using nanobodies as a versatile and innovative alternative to antibodies. The next step will be the validation of the assay in clinical and epidemiological contexts