A novel planning approach for the water, sanitation and hygiene (WaSH) sector: the use of object-oriented bayesian networks

Conventional approaches to design and plan water, sanitation, and hygiene (WaSH) interventions are not suitable for capturing the increasing complexity of the context in which these services are delivered. Multidimensional tools are needed to unravel the links between access to basic services and the socio-economic drivers of poverty. This paper applies an object-oriented Bayesian network to reflect the main issues that determine access to WaSH services. A national Program in Kenya has been analyzed as initial case study. The main findings suggest that the proposed approach is able to accommodate local conditions and to represent an accurate reflection of the complexities of WaSH issues, incorporating the uncertainty intrinsic to service delivery processes. Results indicate those areas in which policy makers should prioritize efforts and resources. Similarly, the study shows the effects of sector interventions, as well as the foreseen impact of various scenarios related to the national Program.Preprin

Mathematical model of a telomerase transcriptional regulatory network developed by cell-based screening: analysis of inhibitor effects and telomerase expression mechanisms

Cancer cells depend on transcription of telomerase reverse transcriptase (TERT). Many transcription factors affect TERT, though regulation occurs in context of a broader network. Network effects on telomerase regulation have not been investigated, though deeper understanding of TERT transcription requires a systems view. However, control over individual interactions in complex networks is not easily achievable. Mathematical modelling provides an attractive approach for analysis of complex systems and some models may prove useful in systems pharmacology approaches to drug discovery. In this report, we used transfection screening to test interactions among 14 TERT regulatory transcription factors and their respective promoters in ovarian cancer cells. The results were used to generate a network model of TERT transcription and to implement a dynamic Boolean model whose steady states were analysed. Modelled effects of signal transduction inhibitors successfully predicted TERT repression by Src-family inhibitor SU6656 and lack of repression by ERK inhibitor FR180204, results confirmed by RT-QPCR analysis of endogenous TERT expression in treated cells. Modelled effects of GSK3 inhibitor 6-bromoindirubin-3′-oxime (BIO) predicted unstable TERT repression dependent on noise and expression of JUN, corresponding with observations from a previous study. MYC expression is critical in TERT activation in the model, consistent with its well known function in endogenous TERT regulation. Loss of MYC caused complete TERT suppression in our model, substantially rescued only by co-suppression of AR. Interestingly expression was easily rescued under modelled Ets-factor gain of function, as occurs in TERT promoter mutation. RNAi targeting AR, JUN, MXD1, SP3, or TP53, showed that AR suppression does rescue endogenous TERT expression following MYC knockdown in these cells and SP3 or TP53 siRNA also cause partial recovery. The model therefore successfully predicted several aspects of TERT regulation including previously unknown mechanisms. An extrapolation suggests that a dominant stimulatory system may programme TERT for transcriptional stability

The "MIND" Scalable PIM Architecture

MIND (Memory, Intelligence, and Network Device) is an advanced parallel computer architecture for high performance computing and scalable embedded processing. It is a Processor-in-Memory (PIM) architecture integrating both DRAM bit cells and CMOS logic devices on the same silicon die. MIND is multicore with multiple memory/processor nodes on each chip and supports global shared memory across systems of MIND components. MIND is distinguished from other PIM architectures in that it incorporates mechanisms for efficient support of a global parallel execution model based on the semantics of message-driven multithreaded split-transaction processing. MIND is designed to operate either in conjunction with other conventional microprocessors or in standalone arrays of like devices. It also incorporates mechanisms for fault tolerance, real time execution, and active power management. This paper describes the major elements and operational methods of the MIND architecture

Verifiably-safe software-defined networks for CPS

Next generation cyber-physical systems (CPS) are expected to be deployed in domains which require scalability as well as performance under dynamic conditions. This scale and dynamicity will require that CPS communication networks be programmatic (i.e., not requiring manual intervention at any stage), but still maintain iron-clad safety guarantees. Software-defined networking standards like OpenFlow provide a means for scalably building tailor-made network architectures, but there is no guarantee that these systems are safe, correct, or secure. In this work we propose a methodology and accompanying tools for specifying and modeling distributed systems such that existing formal verification techniques can be transparently used to analyze critical requirements and properties prior to system implementation. We demonstrate this methodology by iteratively modeling and verifying an OpenFlow learning switch network with respect to network correctness, network convergence, and mobility-related properties. We posit that a design strategy based on the complementary pairing of software-defined networking and formal verification would enable the CPS community to build next-generation systems without sacrificing the safety and reliability that these systems must deliver

Real-time support for high performance aircraft operation

The feasibility of real-time processing schemes using artificial neural networks (ANNs) is investigated. A rationale for digital neural nets is presented and a general processor architecture for control applications is illustrated. Research results on ANN structures for real-time applications are given. Research results on ANN algorithms for real-time control are also shown