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Meta-analysis of massively parallel reporter assays enables prediction of regulatory function across cell types.
Deciphering the potential of noncoding loci to influence gene regulation has been the subject of intense research, with important implications in understanding genetic underpinnings of human diseases. Massively parallel reporter assays (MPRAs) can measure regulatory activity of thousands of DNA sequences and their variants in a single experiment. With increasing number of publically available MPRA data sets, one can now develop data-driven models which, given a DNA sequence, predict its regulatory activity. Here, we performed a comprehensive meta-analysis of several MPRA data sets in a variety of cellular contexts. We first applied an ensemble of methods to predict MPRA output in each context and observed that the most predictive features are consistent across data sets. We then demonstrate that predictive models trained in one cellular context can be used to predict MPRA output in another, with loss of accuracy attributed to cell-type-specific features. Finally, we show that our approach achieves top performance in the Fifth Critical Assessment of Genome Interpretation "Regulation Saturation" Challenge for predicting effects of single-nucleotide variants. Overall, our analysis provides insights into how MPRA data can be leveraged to highlight functional regulatory regions throughout the genome and can guide effective design of future experiments by better prioritizing regions of interest
Feature-based time-series analysis
This work presents an introduction to feature-based time-series analysis. The
time series as a data type is first described, along with an overview of the
interdisciplinary time-series analysis literature. I then summarize the range
of feature-based representations for time series that have been developed to
aid interpretable insights into time-series structure. Particular emphasis is
given to emerging research that facilitates wide comparison of feature-based
representations that allow us to understand the properties of a time-series
dataset that make it suited to a particular feature-based representation or
analysis algorithm. The future of time-series analysis is likely to embrace
approaches that exploit machine learning methods to partially automate human
learning to aid understanding of the complex dynamical patterns in the time
series we measure from the world.Comment: 28 pages, 9 figure
Machine Analysis of Facial Expressions
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