85,701 research outputs found
Position specific variation in the rate of evolution in transcription factor binding sites
BACKGROUND: The binding sites of sequence specific transcription factors are an important and relatively well-understood class of functional non-coding DNAs. Although a wide variety of experimental and computational methods have been developed to characterize transcription factor binding sites, they remain difficult to identify. Comparison of non-coding DNA from related species has shown considerable promise in identifying these functional non-coding sequences, even though relatively little is known about their evolution. RESULTS: Here we analyse the genome sequences of the budding yeasts Saccharomyces cerevisiae, S. bayanus, S. paradoxus and S. mikatae to study the evolution of transcription factor binding sites. As expected, we find that both experimentally characterized and computationally predicted binding sites evolve slower than surrounding sequence, consistent with the hypothesis that they are under purifying selection. We also observe position-specific variation in the rate of evolution within binding sites. We find that the position-specific rate of evolution is positively correlated with degeneracy among binding sites within S. cerevisiae. We test theoretical predictions for the rate of evolution at positions where the base frequencies deviate from background due to purifying selection and find reasonable agreement with the observed rates of evolution. Finally, we show how the evolutionary characteristics of real binding motifs can be used to distinguish them from artefacts of computational motif finding algorithms. CONCLUSION: As has been observed for protein sequences, the rate of evolution in transcription factor binding sites varies with position, suggesting that some regions are under stronger functional constraint than others. This variation likely reflects the varying importance of different positions in the formation of the protein-DNA complex. The characterization of the pattern of evolution in known binding sites will likely contribute to the effective use of comparative sequence data in the identification of transcription factor binding sites and is an important step toward understanding the evolution of functional non-coding DNA
Formation of regulatory modules by local sequence duplication
Turnover of regulatory sequence and function is an important part of
molecular evolution. But what are the modes of sequence evolution leading to
rapid formation and loss of regulatory sites? Here, we show that a large
fraction of neighboring transcription factor binding sites in the fly genome
have formed from a common sequence origin by local duplications. This mode of
evolution is found to produce regulatory information: duplications can seed new
sites in the neighborhood of existing sites. Duplicate seeds evolve
subsequently by point mutations, often towards binding a different factor than
their ancestral neighbor sites. These results are based on a statistical
analysis of 346 cis-regulatory modules in the Drosophila melanogaster genome,
and a comparison set of intergenic regulatory sequence in Saccharomyces
cerevisiae. In fly regulatory modules, pairs of binding sites show
significantly enhanced sequence similarity up to distances of about 50 bp. We
analyze these data in terms of an evolutionary model with two distinct modes of
site formation: (i) evolution from independent sequence origin and (ii)
divergent evolution following duplication of a common ancestor sequence. Our
results suggest that pervasive formation of binding sites by local sequence
duplications distinguishes the complex regulatory architecture of higher
eukaryotes from the simpler architecture of unicellular organisms
Extensive divergence of transcription factor binding in Drosophila embryos with highly conserved gene expression
Extensive divergence of transcription factor binding in Drosophila embryos
with highly conserved gene expressionComment: 7 figures, 20 supplementary figures, 6 supplementary tables Paris M,
Kaplan T, Li XY, Villalta JE, Lott SE, et al. (2013) Extensive Divergence of
Transcription Factor Binding in Drosophila Embryos with Highly Conserved Gene
Expression. PLoS Genet 9(9): e1003748. doi:10.1371/journal.pgen.100374
Evolution of new regulatory functions on biophysically realistic fitness landscapes
Regulatory networks consist of interacting molecules with a high degree of
mutual chemical specificity. How can these molecules evolve when their function
depends on maintenance of interactions with cognate partners and simultaneous
avoidance of deleterious "crosstalk" with non-cognate molecules? Although
physical models of molecular interactions provide a framework in which
co-evolution of network components can be analyzed, most theoretical studies
have focused on the evolution of individual alleles, neglecting the network. In
contrast, we study the elementary step in the evolution of gene regulatory
networks: duplication of a transcription factor followed by selection for TFs
to specialize their inputs as well as the regulation of their downstream genes.
We show how to coarse grain the complete, biophysically realistic
genotype-phenotype map for this process into macroscopic functional outcomes
and quantify the probability of attaining each. We determine which evolutionary
and biophysical parameters bias evolutionary trajectories towards fast
emergence of new functions and show that this can be greatly facilitated by the
availability of "promiscuity-promoting" mutations that affect TF specificity
Functional Bias and Spatial Organization of Genes in Mutational Hot and Cold Regions in the Human Genome
The neutral mutation rate is known to vary widely along human chromosomes,
leading to mutational hot and cold regions. We provide evidence that categories
of functionally-related genes reside preferentially in mutationally hot or cold
regions, the size of which we have measured. Genes in hot regions are biased
toward extra-cellular communication (surface receptors, cell adhesion, immune
response, etc.) while those in cold regions are biased toward essential
cellular processes (gene regulation, RNA processing, protein modification,
etc.). From a selective perspective, this organization of genes could minimize
the mutational load on genes that need to be conserved and allow fast evolution
for genes that must frequently adapt. We also analyze the effect of gene
duplication and chromosomal recombination, which contribute significantly to
these biases for certain categories of hot genes. Overall, our results show
that genes are located non-randomly with respect to hot and cold regions,
offering the possibility that selection acts at the level of gene location in
the human genome.Comment: 17 pages, 6 figures, 2 tables. accepted to PLOS Biology, Feb. 2004
issu
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Transfer RNA genes experience exceptionally elevated mutation rates.
Transfer RNAs (tRNAs) are a central component for the biological synthesis of proteins, and they are among the most highly conserved and frequently transcribed genes in all living things. Despite their clear significance for fundamental cellular processes, the forces governing tRNA evolution are poorly understood. We present evidence that transcription-associated mutagenesis and strong purifying selection are key determinants of patterns of sequence variation within and surrounding tRNA genes in humans and diverse model organisms. Remarkably, the mutation rate at broadly expressed cytosolic tRNA loci is likely between 7 and 10 times greater than the nuclear genome average. Furthermore, evolutionary analyses provide strong evidence that tRNA genes, but not their flanking sequences, experience strong purifying selection acting against this elevated mutation rate. We also find a strong correlation between tRNA expression levels and the mutation rates in their immediate flanking regions, suggesting a simple method for estimating individual tRNA gene activity. Collectively, this study illuminates the extreme competing forces in tRNA gene evolution and indicates that mutations at tRNA loci contribute disproportionately to mutational load and have unexplored fitness consequences in human populations
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Evolution of substrate-specific gene expression and RNA editing in brown rot wood-decaying fungi.
Fungi that decay wood have characteristic associations with certain tree species, but the mechanistic bases for these associations are poorly understood. We studied substrate-specific gene expression and RNA editing in six species of wood-decaying fungi from the 'Antrodia clade' (Polyporales, Agaricomycetes) on three different wood substrates (pine, spruce, and aspen) in submerged cultures. We identified dozens to hundreds of substrate-biased genes (i.e., genes that are significantly upregulated in one substrate relative to the other two substrates) in each species, and these biased genes are correlated with their host ranges. Evolution of substrate-biased genes is associated with gene family expansion, gain and loss of genes, and variation in cis- and trans- regulatory elements, rather than changes in protein coding sequences. We also demonstrated widespread RNA editing events in the Antrodia clade, which differ from those observed in the Ascomycota in their distribution, substitution types, and the genomic environment. Moreover, we found that substrates could affect editing positions and frequency, including editing events occurring in mRNA transcribed from wood-decay-related genes. This work shows the extent to which gene expression and RNA editing differ among species and substrates, and provides clues into mechanisms by which wood-decaying fungi may adapt to different hosts
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