3D exemplar-based image inpainting in electron microscopy

In electron microscopy (EM) a common problem is the non-availability of data, which causes artefacts in reconstructions. In this thesis the goal is to generate artificial data where missing in EM by using exemplar-based inpainting (EBI). We implement an accelerated 3D version tailored to applications in EM, which reduces reconstruction times from days to minutes. We develop intelligent sampling strategies to find optimal data as input for reconstruction methods. Further, we investigate approaches to reduce electron dose and acquisition time. Sparse sampling followed by inpainting is the most promising approach. As common evaluation measures may lead to misinterpretation of results in EM and falsify a subsequent analysis, we propose to use application driven metrics and demonstrate this in a segmentation task. A further application of our technique is the artificial generation of projections in tiltbased EM. EBI is used to generate missing projections, such that the full angular range is covered. Subsequent reconstructions are significantly enhanced in terms of resolution, which facilitates further analysis of samples. In conclusion, EBI proves promising when used as an additional data generation step to tackle the non-availability of data in EM, which is evaluated in selected applications. Enhancing adaptive sampling methods and refining EBI, especially considering the mutual influence, promotes higher throughput in EM using less electron dose while not lessening quality.Ein häufig vorkommendes Problem in der Elektronenmikroskopie (EM) ist die Nichtverfügbarkeit von Daten, was zu Artefakten in Rekonstruktionen führt. In dieser Arbeit ist es das Ziel fehlende Daten in der EM künstlich zu erzeugen, was durch Exemplar-basiertes Inpainting (EBI) realisiert wird. Wir implementieren eine auf EM zugeschnittene beschleunigte 3D Version, welche es ermöglicht, Rekonstruktionszeiten von Tagen auf Minuten zu reduzieren. Wir entwickeln intelligente Abtaststrategien, um optimale Datenpunkte für die Rekonstruktion zu erhalten. Ansätze zur Reduzierung von Elektronendosis und Aufnahmezeit werden untersucht. Unterabtastung gefolgt von Inpainting führt zu den besten Resultaten. Evaluationsmaße zur Beurteilung der Rekonstruktionsqualität helfen in der EM oft nicht und können zu falschen Schlüssen führen, weswegen anwendungsbasierte Metriken die bessere Wahl darstellen. Dies demonstrieren wir anhand eines Beispiels. Die künstliche Erzeugung von Projektionen in der neigungsbasierten Elektronentomographie ist eine weitere Anwendung. EBI wird verwendet um fehlende Projektionen zu generieren. Daraus resultierende Rekonstruktionen weisen eine deutlich erhöhte Auflösung auf. EBI ist ein vielversprechender Ansatz, um nicht verfügbare Daten in der EM zu generieren. Dies wird auf Basis verschiedener Anwendungen gezeigt und evaluiert. Adaptive Aufnahmestrategien und EBI können also zu einem höheren Durchsatz in der EM führen, ohne die Bildqualität merklich zu verschlechtern

On Martian Surface Exploration: Development of Automated 3D Reconstruction and Super-Resolution Restoration Techniques for Mars Orbital Images

Very high spatial resolution imaging and topographic (3D) data play an important role in modern Mars science research and engineering applications. This work describes a set of image processing and machine learning methods to produce the “best possible” high-resolution and high-quality 3D and imaging products from existing Mars orbital imaging datasets. The research work is described in nine chapters of which seven are based on separate published journal papers. These include a) a hybrid photogrammetric processing chain that combines the advantages of different stereo matching algorithms to compute stereo disparity with optimal completeness, fine-scale details, and minimised matching artefacts; b) image and 3D co-registration methods that correct a target image and/or 3D data to a reference image and/or 3D data to achieve robust cross-instrument multi-resolution 3D and image co-alignment; c) a deep learning network and processing chain to estimate pixel-scale surface topography from single-view imagery that outperforms traditional photogrammetric methods in terms of product quality and processing speed; d) a deep learning-based single-image super-resolution restoration (SRR) method to enhance the quality and effective resolution of Mars orbital imagery; e) a subpixel-scale 3D processing system using a combination of photogrammetric 3D reconstruction, SRR, and photoclinometric 3D refinement; and f) an optimised subpixel-scale 3D processing system using coupled deep learning based single-view SRR and deep learning based 3D estimation to derive the best possible (in terms of visual quality, effective resolution, and accuracy) 3D products out of present epoch Mars orbital images. The resultant 3D imaging products from the above listed new developments are qualitatively and quantitatively evaluated either in comparison with products from the official NASA planetary data system (PDS) and/or ESA planetary science archive (PSA) releases, and/or in comparison with products generated with different open-source systems. Examples of the scientific application of these novel 3D imaging products are discussed

High-throughput molecular imaging via deep-learning-enabled Raman spectroscopy.

Raman spectroscopy enables nondestructive, label-free imaging with unprecedented molecular contrast, but is limited by slow data acquisition, largely preventing high-throughput imaging applications. Here, we present a comprehensive framework for higher-throughput molecular imaging via deep-learning-enabled Raman spectroscopy, termed DeepeR, trained on a large data set of hyperspectral Raman images, with over 1.5 million spectra (400 h of acquisition) in total. We first perform denoising and reconstruction of low signal-to-noise ratio Raman molecular signatures via deep learning, with a 10× improvement in the mean-squared error over common Raman filtering methods. Next, we develop a neural network for robust 2-4× spatial super-resolution of hyperspectral Raman images that preserve molecular cellular information. Combining these approaches, we achieve Raman imaging speed-ups of up to 40-90×, enabling good-quality cellular imaging with a high-resolution, high signal-to-noise ratio in under 1 min. We further demonstrate Raman imaging speed-up of 160×, useful for lower resolution imaging applications such as the rapid screening of large areas or for spectral pathology. Finally, transfer learning is applied to extend DeepeR from cell to tissue-scale imaging. DeepeR provides a foundation that will enable a host of higher-throughput Raman spectroscopy and molecular imaging applications across biomedicine

Dense light field coding: a survey

Light Field (LF) imaging is a promising solution for providing more immersive and closer to reality multimedia experiences to end-users with unprecedented creative freedom and flexibility for applications in different areas, such as virtual and augmented reality. Due to the recent technological advances in optics, sensor manufacturing and available transmission bandwidth, as well as the investment of many tech giants in this area, it is expected that soon many LF transmission systems will be available to both consumers and professionals. Recognizing this, novel standardization initiatives have recently emerged in both the Joint Photographic Experts Group (JPEG) and the Moving Picture Experts Group (MPEG), triggering the discussion on the deployment of LF coding solutions to efficiently handle the massive amount of data involved in such systems. Since then, the topic of LF content coding has become a booming research area, attracting the attention of many researchers worldwide. In this context, this paper provides a comprehensive survey of the most relevant LF coding solutions proposed in the literature, focusing on angularly dense LFs. Special attention is placed on a thorough description of the different LF coding methods and on the main concepts related to this relevant area. Moreover, comprehensive insights are presented into open research challenges and future research directions for LF coding.info:eu-repo/semantics/publishedVersio